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Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats.

Lee YH, Chang JJ, Chien CT, Yang MC, Chien HF - Exp Diabetes Res (2012)

Bottom Line: In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline.After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation.This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.

ABSTRACT
We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

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Related in: MedlinePlus

The effect of saline, Pluronic F127, antioxidant saline, and antioxidant Pluronic F127 on wound closure in the normal rats (a) and diabetic rats (b). Full-thickness skin wounds of 1.0 × 1.0 cm were measured from the time of wounding until closures. The skin defect was compared to the initial wound size to determine wound closure rate by tracing the wound. (a) Closure of full-thickness skin wounds of normal rats showed no significant difference in the healing rate between four groups of treatment. (b) Wound closure of diabetic skin showed that the antioxidant sol-gel-treated wounds closed faster than the saline-, sol- and antioxidant saline-treated wounds on days 7 and 14. Data is expressed as mean ± SEM for three separate experiments, each in quadruplicate. *P < 0.05 when compared to saline control. (c) Representative pictures of skin wounds in group saline (a), Pluronic F127 (b), antioxidant saline (c), and antioxidant sol-gel (d) at day 7.
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fig2: The effect of saline, Pluronic F127, antioxidant saline, and antioxidant Pluronic F127 on wound closure in the normal rats (a) and diabetic rats (b). Full-thickness skin wounds of 1.0 × 1.0 cm were measured from the time of wounding until closures. The skin defect was compared to the initial wound size to determine wound closure rate by tracing the wound. (a) Closure of full-thickness skin wounds of normal rats showed no significant difference in the healing rate between four groups of treatment. (b) Wound closure of diabetic skin showed that the antioxidant sol-gel-treated wounds closed faster than the saline-, sol- and antioxidant saline-treated wounds on days 7 and 14. Data is expressed as mean ± SEM for three separate experiments, each in quadruplicate. *P < 0.05 when compared to saline control. (c) Representative pictures of skin wounds in group saline (a), Pluronic F127 (b), antioxidant saline (c), and antioxidant sol-gel (d) at day 7.

Mentions: Rats receiving STZ have significant elevation of blood glucose level (>300 mg/dL) after 3 weeks, which was sustained throughout the duration of the study. The wound healing of various treatments was evaluated in a full-thickness wound model. The wounds decreased in size gradually with time, closed at 2 weeks in normal rats and at 3 weeks in diabetic rats. We did not note any statistical difference in the wound closure of the normal rats with four kinds of treatment (Figure 2(a)). This was not unexpected since skin wounds of rats are known to heal efficiently and there is little room for improvement. However, in the diabetic rats, periodical observation of animals at 7 days showed a significant increase (P < 0.05) in the rate of contraction of wounds in the antioxidant sol-gel and antioxidant saline groups when compared to saline group (Figure 2(b)). In the diabetic rats at 14 days, a significant (P < 0.05) wound closure was noted in the pluronic F127, antioxidant sol-gel, and antioxidant saline groups when compared to saline group (Figure 2(b)). However, the wound closure rate was displayed in a tendency of antioxidant sol-gel > antioxidant saline = Pluronic F127 ≥ saline in the diabetic wounds at 14 days after wounding. However, all groups attained full closure by the end of the third week.


Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats.

Lee YH, Chang JJ, Chien CT, Yang MC, Chien HF - Exp Diabetes Res (2012)

The effect of saline, Pluronic F127, antioxidant saline, and antioxidant Pluronic F127 on wound closure in the normal rats (a) and diabetic rats (b). Full-thickness skin wounds of 1.0 × 1.0 cm were measured from the time of wounding until closures. The skin defect was compared to the initial wound size to determine wound closure rate by tracing the wound. (a) Closure of full-thickness skin wounds of normal rats showed no significant difference in the healing rate between four groups of treatment. (b) Wound closure of diabetic skin showed that the antioxidant sol-gel-treated wounds closed faster than the saline-, sol- and antioxidant saline-treated wounds on days 7 and 14. Data is expressed as mean ± SEM for three separate experiments, each in quadruplicate. *P < 0.05 when compared to saline control. (c) Representative pictures of skin wounds in group saline (a), Pluronic F127 (b), antioxidant saline (c), and antioxidant sol-gel (d) at day 7.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3420222&req=5

fig2: The effect of saline, Pluronic F127, antioxidant saline, and antioxidant Pluronic F127 on wound closure in the normal rats (a) and diabetic rats (b). Full-thickness skin wounds of 1.0 × 1.0 cm were measured from the time of wounding until closures. The skin defect was compared to the initial wound size to determine wound closure rate by tracing the wound. (a) Closure of full-thickness skin wounds of normal rats showed no significant difference in the healing rate between four groups of treatment. (b) Wound closure of diabetic skin showed that the antioxidant sol-gel-treated wounds closed faster than the saline-, sol- and antioxidant saline-treated wounds on days 7 and 14. Data is expressed as mean ± SEM for three separate experiments, each in quadruplicate. *P < 0.05 when compared to saline control. (c) Representative pictures of skin wounds in group saline (a), Pluronic F127 (b), antioxidant saline (c), and antioxidant sol-gel (d) at day 7.
Mentions: Rats receiving STZ have significant elevation of blood glucose level (>300 mg/dL) after 3 weeks, which was sustained throughout the duration of the study. The wound healing of various treatments was evaluated in a full-thickness wound model. The wounds decreased in size gradually with time, closed at 2 weeks in normal rats and at 3 weeks in diabetic rats. We did not note any statistical difference in the wound closure of the normal rats with four kinds of treatment (Figure 2(a)). This was not unexpected since skin wounds of rats are known to heal efficiently and there is little room for improvement. However, in the diabetic rats, periodical observation of animals at 7 days showed a significant increase (P < 0.05) in the rate of contraction of wounds in the antioxidant sol-gel and antioxidant saline groups when compared to saline group (Figure 2(b)). In the diabetic rats at 14 days, a significant (P < 0.05) wound closure was noted in the pluronic F127, antioxidant sol-gel, and antioxidant saline groups when compared to saline group (Figure 2(b)). However, the wound closure rate was displayed in a tendency of antioxidant sol-gel > antioxidant saline = Pluronic F127 ≥ saline in the diabetic wounds at 14 days after wounding. However, all groups attained full closure by the end of the third week.

Bottom Line: In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline.After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation.This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.

ABSTRACT
We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

Show MeSH
Related in: MedlinePlus