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Brain Levels of Catalase Remain Constant through Strain, Developmental, and Chronic Alcohol Challenges.

Rhoads DE, Contreras C, Fathalla S - Enzyme Res (2012)

Bottom Line: The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings.Levels of catalase appear to have achieved the adult level by the preadolescent period defined by postnatal age (P, days) P25-P28, and there were no differences between strains at the developmental stages tested.Catalase activities remained unchanged following ethanol consumption, with no significant differences within or between strains.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Monmouth University, West Long Branch, NJ 07764, USA.

ABSTRACT
Catalase (EC 1.11.1.6) oxidizes ethanol to acetaldehyde within the brain and variations in catalase activity may underlie some consequences of ethanol consumption. The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings. In the first series of studies, levels of catalase were compared between juvenile and adult rats and between the Long-Evans (LE) and Sprague-Dawley (SD) strains. Levels of catalase appear to have achieved the adult level by the preadolescent period defined by postnatal age (P, days) P25-P28, and there were no differences between strains at the developmental stages tested. Thus, variation in catalase activity is unlikely to be responsible for differences in how adolescent and adult rats respond to ethanol. In the second series of studies, periadolescent and adult rats were administered ethanol chronically through an ethanol-containing liquid diet. Diet consumption and blood ethanol concentrations were significantly higher for periadolescent rats. Catalase activities remained unchanged following ethanol consumption, with no significant differences within or between strains. Thus, the brain showed no apparent adaptive changes in levels of catalase, even when faced with the high levels of ethanol consumption characteristic of periadolescent rats.

No MeSH data available.


Related in: MedlinePlus

Blood ethanol concentrations were significantly higher for periadolescent rats. Following three weeks of consuming an ethanol-containing liquid diet, Long-Evans and Sprague-Dawley rats which began diet consumption as preadolescents at postnatal age 25–28 days (P25–28) or as adults (P75 or greater) were sacrificed, and blood ethanol concentration (BEC) was determined in trunk blood and presented as mean ± SEM. *For each strain, there was a highly significant difference in BEC between the periadolescent rats and adults (P = 0.003).
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fig2: Blood ethanol concentrations were significantly higher for periadolescent rats. Following three weeks of consuming an ethanol-containing liquid diet, Long-Evans and Sprague-Dawley rats which began diet consumption as preadolescents at postnatal age 25–28 days (P25–28) or as adults (P75 or greater) were sacrificed, and blood ethanol concentration (BEC) was determined in trunk blood and presented as mean ± SEM. *For each strain, there was a highly significant difference in BEC between the periadolescent rats and adults (P = 0.003).

Mentions: Blood ethanol concentrations (BECs) appeared to be consistent with the differences in alcohol consumption between adults and adolescents of each strain (Figure 2). Two-factor ANOVA again yielded a significant main effect of developmental stage (F(1,20) = 11.126, P = 0.003) with BEC significantly higher for adolescents than adults. There was no significant effect of strain (F(1,20) = 0.001, P = 0.97) and no interaction effect between strain and developmental stage (F(1,20) = 0.090, P = 0.768) on BEC.


Brain Levels of Catalase Remain Constant through Strain, Developmental, and Chronic Alcohol Challenges.

Rhoads DE, Contreras C, Fathalla S - Enzyme Res (2012)

Blood ethanol concentrations were significantly higher for periadolescent rats. Following three weeks of consuming an ethanol-containing liquid diet, Long-Evans and Sprague-Dawley rats which began diet consumption as preadolescents at postnatal age 25–28 days (P25–28) or as adults (P75 or greater) were sacrificed, and blood ethanol concentration (BEC) was determined in trunk blood and presented as mean ± SEM. *For each strain, there was a highly significant difference in BEC between the periadolescent rats and adults (P = 0.003).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3420129&req=5

fig2: Blood ethanol concentrations were significantly higher for periadolescent rats. Following three weeks of consuming an ethanol-containing liquid diet, Long-Evans and Sprague-Dawley rats which began diet consumption as preadolescents at postnatal age 25–28 days (P25–28) or as adults (P75 or greater) were sacrificed, and blood ethanol concentration (BEC) was determined in trunk blood and presented as mean ± SEM. *For each strain, there was a highly significant difference in BEC between the periadolescent rats and adults (P = 0.003).
Mentions: Blood ethanol concentrations (BECs) appeared to be consistent with the differences in alcohol consumption between adults and adolescents of each strain (Figure 2). Two-factor ANOVA again yielded a significant main effect of developmental stage (F(1,20) = 11.126, P = 0.003) with BEC significantly higher for adolescents than adults. There was no significant effect of strain (F(1,20) = 0.001, P = 0.97) and no interaction effect between strain and developmental stage (F(1,20) = 0.090, P = 0.768) on BEC.

Bottom Line: The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings.Levels of catalase appear to have achieved the adult level by the preadolescent period defined by postnatal age (P, days) P25-P28, and there were no differences between strains at the developmental stages tested.Catalase activities remained unchanged following ethanol consumption, with no significant differences within or between strains.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Monmouth University, West Long Branch, NJ 07764, USA.

ABSTRACT
Catalase (EC 1.11.1.6) oxidizes ethanol to acetaldehyde within the brain and variations in catalase activity may underlie some consequences of ethanol consumption. The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings. In the first series of studies, levels of catalase were compared between juvenile and adult rats and between the Long-Evans (LE) and Sprague-Dawley (SD) strains. Levels of catalase appear to have achieved the adult level by the preadolescent period defined by postnatal age (P, days) P25-P28, and there were no differences between strains at the developmental stages tested. Thus, variation in catalase activity is unlikely to be responsible for differences in how adolescent and adult rats respond to ethanol. In the second series of studies, periadolescent and adult rats were administered ethanol chronically through an ethanol-containing liquid diet. Diet consumption and blood ethanol concentrations were significantly higher for periadolescent rats. Catalase activities remained unchanged following ethanol consumption, with no significant differences within or between strains. Thus, the brain showed no apparent adaptive changes in levels of catalase, even when faced with the high levels of ethanol consumption characteristic of periadolescent rats.

No MeSH data available.


Related in: MedlinePlus