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The Association between Y Chromosome Microdeletion and Recurrent Pregnancy Loss.

Ghorbian S, Saliminejad K, Sadeghi MR, Javadi GR, Kamali K, Amirjannati N, Bahreini F, Edalatkhah H, Khorram Khorshid HR - Iran Red Crescent Med J (2012)

Bottom Line: Therefore, the current study was designed to examine whether Y chromosome microdeletions were associated with RPL in an Iranian population.Genomic DNA was extracted from peripheral blood and tested for Y chromosome microdeletions in AZFa, AZFb and AZFc regions using two multiplex PCR.None of the men in the case and control groups had any microdeletions in the AZFa, AZFb and AZFc regions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: To date, the role of male factor contributing in evaluation of spontaneous recurrent pregnancy loss (RPL) has been less investigated and there is discrepancy in the role of Y chromosome microdeltions in RPL. Therefore, the current study was designed to examine whether Y chromosome microdeletions were associated with RPL in an Iranian population.

Methods: One hundred men from couples, experiencing three or more RPLs, and one hundred normal men from couples with at least one child and no history of miscarriages as control group were included. Genomic DNA was extracted from peripheral blood and tested for Y chromosome microdeletions in AZFa, AZFb and AZFc regions using two multiplex PCR.

Results: None of the men in the case and control groups had any microdeletions in the AZFa, AZFb and AZFc regions.

Conclusion: It seems that Y chromosome microdeletion is not associated with recurrent pregnancy loss, therefore performing this test in Iranian couples with RPL is not recommended.

No MeSH data available.


Related in: MedlinePlus

The result of the multiplex A and B. PCR fragments were separated on 3% agarose gel. Lanes 1-2-3, men with couples experiencing RPL; Lanes 4-5, fertile control men; M, Molecular weight marker (100 bp ladder); Cont -, Negative control (Water). SRY (472 bp), sY84 (324 bp), sY86 (326 bp), sY127 (274 bp), sY134 (301 bp), sY254 (380 bp), sY255 (123 bp), sY150 (158 bp), sY152 (125 bp).
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s2fig1: The result of the multiplex A and B. PCR fragments were separated on 3% agarose gel. Lanes 1-2-3, men with couples experiencing RPL; Lanes 4-5, fertile control men; M, Molecular weight marker (100 bp ladder); Cont -, Negative control (Water). SRY (472 bp), sY84 (324 bp), sY86 (326 bp), sY127 (274 bp), sY134 (301 bp), sY254 (380 bp), sY255 (123 bp), sY150 (158 bp), sY152 (125 bp).

Mentions: Multiplex a reaction contains sY86, sY127, sY254 and sY152 while multiplex B reaction contains sY84, sY134, Y255 and sY150. The SRY gene was used as internal control in both reactions. The sequences of all primer pairs and the size of the PCR products were shown in Table 1. The PCR was carried out in a total volume of 25 µl containing 200 ng of genomic DNA, 1xPCR buffer, 4 mM MgCl2, 1U Taq DNA polymerase (Roche; Germany), 10 nmol each NTPs, and 10 pmol of each primers. Amplification conditions were started with an initial denaturation at 95°C for 10 minutes, followed by 40 cycles of denaturation at 94°C for 30 seconds, annealing at 62°C for 45 seconds, and extension at 72°C for 90 seconds, ended by a last extension at 72°C for 10 minutes and cooling to 4°C. The PCR products were separated on 3% Agarose gel, stained with ethidium bromide and visualized under UV light. Genomic DNA from fertile men and water used as positive and negative control, respectively (Figure 1).


The Association between Y Chromosome Microdeletion and Recurrent Pregnancy Loss.

Ghorbian S, Saliminejad K, Sadeghi MR, Javadi GR, Kamali K, Amirjannati N, Bahreini F, Edalatkhah H, Khorram Khorshid HR - Iran Red Crescent Med J (2012)

The result of the multiplex A and B. PCR fragments were separated on 3% agarose gel. Lanes 1-2-3, men with couples experiencing RPL; Lanes 4-5, fertile control men; M, Molecular weight marker (100 bp ladder); Cont -, Negative control (Water). SRY (472 bp), sY84 (324 bp), sY86 (326 bp), sY127 (274 bp), sY134 (301 bp), sY254 (380 bp), sY255 (123 bp), sY150 (158 bp), sY152 (125 bp).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3420026&req=5

s2fig1: The result of the multiplex A and B. PCR fragments were separated on 3% agarose gel. Lanes 1-2-3, men with couples experiencing RPL; Lanes 4-5, fertile control men; M, Molecular weight marker (100 bp ladder); Cont -, Negative control (Water). SRY (472 bp), sY84 (324 bp), sY86 (326 bp), sY127 (274 bp), sY134 (301 bp), sY254 (380 bp), sY255 (123 bp), sY150 (158 bp), sY152 (125 bp).
Mentions: Multiplex a reaction contains sY86, sY127, sY254 and sY152 while multiplex B reaction contains sY84, sY134, Y255 and sY150. The SRY gene was used as internal control in both reactions. The sequences of all primer pairs and the size of the PCR products were shown in Table 1. The PCR was carried out in a total volume of 25 µl containing 200 ng of genomic DNA, 1xPCR buffer, 4 mM MgCl2, 1U Taq DNA polymerase (Roche; Germany), 10 nmol each NTPs, and 10 pmol of each primers. Amplification conditions were started with an initial denaturation at 95°C for 10 minutes, followed by 40 cycles of denaturation at 94°C for 30 seconds, annealing at 62°C for 45 seconds, and extension at 72°C for 90 seconds, ended by a last extension at 72°C for 10 minutes and cooling to 4°C. The PCR products were separated on 3% Agarose gel, stained with ethidium bromide and visualized under UV light. Genomic DNA from fertile men and water used as positive and negative control, respectively (Figure 1).

Bottom Line: Therefore, the current study was designed to examine whether Y chromosome microdeletions were associated with RPL in an Iranian population.Genomic DNA was extracted from peripheral blood and tested for Y chromosome microdeletions in AZFa, AZFb and AZFc regions using two multiplex PCR.None of the men in the case and control groups had any microdeletions in the AZFa, AZFb and AZFc regions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: To date, the role of male factor contributing in evaluation of spontaneous recurrent pregnancy loss (RPL) has been less investigated and there is discrepancy in the role of Y chromosome microdeltions in RPL. Therefore, the current study was designed to examine whether Y chromosome microdeletions were associated with RPL in an Iranian population.

Methods: One hundred men from couples, experiencing three or more RPLs, and one hundred normal men from couples with at least one child and no history of miscarriages as control group were included. Genomic DNA was extracted from peripheral blood and tested for Y chromosome microdeletions in AZFa, AZFb and AZFc regions using two multiplex PCR.

Results: None of the men in the case and control groups had any microdeletions in the AZFa, AZFb and AZFc regions.

Conclusion: It seems that Y chromosome microdeletion is not associated with recurrent pregnancy loss, therefore performing this test in Iranian couples with RPL is not recommended.

No MeSH data available.


Related in: MedlinePlus