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Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

The dorsal medial MOB projects to olfactory cortical structures from the AON to the ENT (2.345 to –3.98 mm from bregma). Axons ramify in AONd, AONm, AONpv (A), TTd, TTv (B,C), PIR (A–I), OTl (D), AAA (E), NLOT (F), COAa (F–H), COApl (G–I), BA (G), MEAav (H), PAA (H,I), TR (I), and ENTl (J) without any spatial topography. The DP, OTm, COApm, and ENTm do not receive inputs from the dorsal medial MOBmi (B,D,I,J). The PIR-OT junction contains dense labeling (C), where the fibers appear to wrap around layer II pyramidal neurons in OTl (Figures 12H,I). Axon numbers appear to decrease as they travel medially (B–J) and caudally (A–J). Differences between projection patterns from the dorsal medial and ventral lateral MOBmi were not detected (see Figure 10). Case number SW101213-01A. Scale bar, 500 μm. Abbreviations: AONd, dorsal anterior olfactory nucleus; AONm, medial AON; AONpv, posterior ventral AON; TTd, dorsal taenia tecta; TTv, ventral TT; PIR, piriform area; OTl, lateral olfactory tubercle; OTm, medial OT; AAA, anterior amygdalar area; NLOT, nucleus of lateral olfactory tract; COAa, anterior cortical amygdalar area; COApm, posterior medial COA; BA, bed nucleus of accessory olfactory tract; MEAav, anterior ventral medial amygdalar nucleus; PAA, piriform-amygdalar area; TR, postpiriform transition area; ENTl, lateral entorhinal area; ENTm, medial ENT.
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Figure 11: The dorsal medial MOB projects to olfactory cortical structures from the AON to the ENT (2.345 to –3.98 mm from bregma). Axons ramify in AONd, AONm, AONpv (A), TTd, TTv (B,C), PIR (A–I), OTl (D), AAA (E), NLOT (F), COAa (F–H), COApl (G–I), BA (G), MEAav (H), PAA (H,I), TR (I), and ENTl (J) without any spatial topography. The DP, OTm, COApm, and ENTm do not receive inputs from the dorsal medial MOBmi (B,D,I,J). The PIR-OT junction contains dense labeling (C), where the fibers appear to wrap around layer II pyramidal neurons in OTl (Figures 12H,I). Axon numbers appear to decrease as they travel medially (B–J) and caudally (A–J). Differences between projection patterns from the dorsal medial and ventral lateral MOBmi were not detected (see Figure 10). Case number SW101213-01A. Scale bar, 500 μm. Abbreviations: AONd, dorsal anterior olfactory nucleus; AONm, medial AON; AONpv, posterior ventral AON; TTd, dorsal taenia tecta; TTv, ventral TT; PIR, piriform area; OTl, lateral olfactory tubercle; OTm, medial OT; AAA, anterior amygdalar area; NLOT, nucleus of lateral olfactory tract; COAa, anterior cortical amygdalar area; COApm, posterior medial COA; BA, bed nucleus of accessory olfactory tract; MEAav, anterior ventral medial amygdalar nucleus; PAA, piriform-amygdalar area; TR, postpiriform transition area; ENTl, lateral entorhinal area; ENTm, medial ENT.

Mentions: Double coinjections were made into the dorsal medial (BDA; Figure 6A1) and ventral lateral (PHAL; Figure 6A2) MOBmi and fibers from the rostral to caudal regions of the brain were examined. Regardless of their origin, all axons extensively arborize along the molecular Ia sublayer of the olfactory cortex without any spatial topographic specificity. From the lot, fibers from dorsal medial and ventral lateral MOBmi first arborize in the AON, extending across its external, dorsal, lateral, and posterior ventral divisions (Figures 10A, 11A). Caudally, fibers continue into the Ia layer of the PIR, dorsal/ventral taenia tecta (TTd, TTv), but do not project as far mediodorsal as the dorsal peduncular area (DP; Figures 10B, 11B). Axons densely ramify at the juncture between the PIR and the olfactory tubercle (OT; Figures 10C, 11C). Several cases substantiate this PIR-OT junction labeling where it appears that axons from different parts of the MOB, including the MOBmi and glomerular layer (MOBgl; Figures 12A,E,F), extend into layer II and wrap around pyramidal OT neurons (Figures 12B–D,G–I).


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

The dorsal medial MOB projects to olfactory cortical structures from the AON to the ENT (2.345 to –3.98 mm from bregma). Axons ramify in AONd, AONm, AONpv (A), TTd, TTv (B,C), PIR (A–I), OTl (D), AAA (E), NLOT (F), COAa (F–H), COApl (G–I), BA (G), MEAav (H), PAA (H,I), TR (I), and ENTl (J) without any spatial topography. The DP, OTm, COApm, and ENTm do not receive inputs from the dorsal medial MOBmi (B,D,I,J). The PIR-OT junction contains dense labeling (C), where the fibers appear to wrap around layer II pyramidal neurons in OTl (Figures 12H,I). Axon numbers appear to decrease as they travel medially (B–J) and caudally (A–J). Differences between projection patterns from the dorsal medial and ventral lateral MOBmi were not detected (see Figure 10). Case number SW101213-01A. Scale bar, 500 μm. Abbreviations: AONd, dorsal anterior olfactory nucleus; AONm, medial AON; AONpv, posterior ventral AON; TTd, dorsal taenia tecta; TTv, ventral TT; PIR, piriform area; OTl, lateral olfactory tubercle; OTm, medial OT; AAA, anterior amygdalar area; NLOT, nucleus of lateral olfactory tract; COAa, anterior cortical amygdalar area; COApm, posterior medial COA; BA, bed nucleus of accessory olfactory tract; MEAav, anterior ventral medial amygdalar nucleus; PAA, piriform-amygdalar area; TR, postpiriform transition area; ENTl, lateral entorhinal area; ENTm, medial ENT.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
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Figure 11: The dorsal medial MOB projects to olfactory cortical structures from the AON to the ENT (2.345 to –3.98 mm from bregma). Axons ramify in AONd, AONm, AONpv (A), TTd, TTv (B,C), PIR (A–I), OTl (D), AAA (E), NLOT (F), COAa (F–H), COApl (G–I), BA (G), MEAav (H), PAA (H,I), TR (I), and ENTl (J) without any spatial topography. The DP, OTm, COApm, and ENTm do not receive inputs from the dorsal medial MOBmi (B,D,I,J). The PIR-OT junction contains dense labeling (C), where the fibers appear to wrap around layer II pyramidal neurons in OTl (Figures 12H,I). Axon numbers appear to decrease as they travel medially (B–J) and caudally (A–J). Differences between projection patterns from the dorsal medial and ventral lateral MOBmi were not detected (see Figure 10). Case number SW101213-01A. Scale bar, 500 μm. Abbreviations: AONd, dorsal anterior olfactory nucleus; AONm, medial AON; AONpv, posterior ventral AON; TTd, dorsal taenia tecta; TTv, ventral TT; PIR, piriform area; OTl, lateral olfactory tubercle; OTm, medial OT; AAA, anterior amygdalar area; NLOT, nucleus of lateral olfactory tract; COAa, anterior cortical amygdalar area; COApm, posterior medial COA; BA, bed nucleus of accessory olfactory tract; MEAav, anterior ventral medial amygdalar nucleus; PAA, piriform-amygdalar area; TR, postpiriform transition area; ENTl, lateral entorhinal area; ENTm, medial ENT.
Mentions: Double coinjections were made into the dorsal medial (BDA; Figure 6A1) and ventral lateral (PHAL; Figure 6A2) MOBmi and fibers from the rostral to caudal regions of the brain were examined. Regardless of their origin, all axons extensively arborize along the molecular Ia sublayer of the olfactory cortex without any spatial topographic specificity. From the lot, fibers from dorsal medial and ventral lateral MOBmi first arborize in the AON, extending across its external, dorsal, lateral, and posterior ventral divisions (Figures 10A, 11A). Caudally, fibers continue into the Ia layer of the PIR, dorsal/ventral taenia tecta (TTd, TTv), but do not project as far mediodorsal as the dorsal peduncular area (DP; Figures 10B, 11B). Axons densely ramify at the juncture between the PIR and the olfactory tubercle (OT; Figures 10C, 11C). Several cases substantiate this PIR-OT junction labeling where it appears that axons from different parts of the MOB, including the MOBmi and glomerular layer (MOBgl; Figures 12A,E,F), extend into layer II and wrap around pyramidal OT neurons (Figures 12B–D,G–I).

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus