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Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

Connectional differences between PIRa and PIRp. FG injections in dorsal medial(A) and ventral lateral(D) MOBmi back-label neurons in PIRa (B,E), but not in PIRp (C,F). Layer Ia of PIR, labeled with PHAL (see Figure 6A2 for PHAL injection), appears to get thinner in width from PIRa to PIRp (B,C,E–I). Input to PIRa and PIRp is also different as MOB projects more densely to PIRa compared to PIRp (G–I). PIRa between white arrows (G) is magnified in (H), while PIRp in between red arrows (G) is magnified in (I; see Figures 1A,B for PHAL injection). Topographic arrangement of neurons in PIRa (red arrows in B,E) are more clearly observed when FG and CTb are double injected in dorsal versus ventral MOBmi, respectively (J,K). Dorsal MOBmi projecting CTb neurons occupy more dorsal regions of PIRa (L), while ventral projecting neurons are in more ventral parts of PIRa (M). Scale bar, 200 μm; 500 μm (G). Case numbers SW101213-01A (A–C), SW101215-03A (D–F), SW101215-02A (G–I), SW110607-03A (J–M).
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Figure 9: Connectional differences between PIRa and PIRp. FG injections in dorsal medial(A) and ventral lateral(D) MOBmi back-label neurons in PIRa (B,E), but not in PIRp (C,F). Layer Ia of PIR, labeled with PHAL (see Figure 6A2 for PHAL injection), appears to get thinner in width from PIRa to PIRp (B,C,E–I). Input to PIRa and PIRp is also different as MOB projects more densely to PIRa compared to PIRp (G–I). PIRa between white arrows (G) is magnified in (H), while PIRp in between red arrows (G) is magnified in (I; see Figures 1A,B for PHAL injection). Topographic arrangement of neurons in PIRa (red arrows in B,E) are more clearly observed when FG and CTb are double injected in dorsal versus ventral MOBmi, respectively (J,K). Dorsal MOBmi projecting CTb neurons occupy more dorsal regions of PIRa (L), while ventral projecting neurons are in more ventral parts of PIRa (M). Scale bar, 200 μm; 500 μm (G). Case numbers SW101213-01A (A–C), SW101215-03A (D–F), SW101215-02A (G–I), SW110607-03A (J–M).

Mentions: Differential PIRa and PIRp connectivity is substantiated by FG injections in the medial and lateral MOBmi that back-label neurons only in the PIRa and not PIRp (Figures 9A–F). This pattern holds true regardless of the dorsal-ventral position of the MOB injections (data not shown). Our data also suggests that PIRa receives more inputs from MOBmi than the PIRp. From a sagittal view, MOB fiber ramifications decrease as they progress from PIRa to PIRp (Figures 9G–I).


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Connectional differences between PIRa and PIRp. FG injections in dorsal medial(A) and ventral lateral(D) MOBmi back-label neurons in PIRa (B,E), but not in PIRp (C,F). Layer Ia of PIR, labeled with PHAL (see Figure 6A2 for PHAL injection), appears to get thinner in width from PIRa to PIRp (B,C,E–I). Input to PIRa and PIRp is also different as MOB projects more densely to PIRa compared to PIRp (G–I). PIRa between white arrows (G) is magnified in (H), while PIRp in between red arrows (G) is magnified in (I; see Figures 1A,B for PHAL injection). Topographic arrangement of neurons in PIRa (red arrows in B,E) are more clearly observed when FG and CTb are double injected in dorsal versus ventral MOBmi, respectively (J,K). Dorsal MOBmi projecting CTb neurons occupy more dorsal regions of PIRa (L), while ventral projecting neurons are in more ventral parts of PIRa (M). Scale bar, 200 μm; 500 μm (G). Case numbers SW101213-01A (A–C), SW101215-03A (D–F), SW101215-02A (G–I), SW110607-03A (J–M).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3412993&req=5

Figure 9: Connectional differences between PIRa and PIRp. FG injections in dorsal medial(A) and ventral lateral(D) MOBmi back-label neurons in PIRa (B,E), but not in PIRp (C,F). Layer Ia of PIR, labeled with PHAL (see Figure 6A2 for PHAL injection), appears to get thinner in width from PIRa to PIRp (B,C,E–I). Input to PIRa and PIRp is also different as MOB projects more densely to PIRa compared to PIRp (G–I). PIRa between white arrows (G) is magnified in (H), while PIRp in between red arrows (G) is magnified in (I; see Figures 1A,B for PHAL injection). Topographic arrangement of neurons in PIRa (red arrows in B,E) are more clearly observed when FG and CTb are double injected in dorsal versus ventral MOBmi, respectively (J,K). Dorsal MOBmi projecting CTb neurons occupy more dorsal regions of PIRa (L), while ventral projecting neurons are in more ventral parts of PIRa (M). Scale bar, 200 μm; 500 μm (G). Case numbers SW101213-01A (A–C), SW101215-03A (D–F), SW101215-02A (G–I), SW110607-03A (J–M).
Mentions: Differential PIRa and PIRp connectivity is substantiated by FG injections in the medial and lateral MOBmi that back-label neurons only in the PIRa and not PIRp (Figures 9A–F). This pattern holds true regardless of the dorsal-ventral position of the MOB injections (data not shown). Our data also suggests that PIRa receives more inputs from MOBmi than the PIRp. From a sagittal view, MOB fiber ramifications decrease as they progress from PIRa to PIRp (Figures 9G–I).

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus