Limits...
Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

Schematic of lateral and medial MOB projections to lot. Colors of injections in (A) and lot fibers in (B) are linked. Axons from dorsal (blue) or ventral (purple) lateral MOB travel directly toward the lot on the same side (A) and remain approximately in the dorsal and ventral lot, respectively (B). Fibers from dorsal medial MOB (orange) travel through the lotd, while those from ventral medial MOB (pink) take a ventrolateral route. Fibers originating from the middle medial MOB (green) travel either through the lotd or ventrolaterally toward lot. Within the lot, dorsal medial MOB fibers stay restricted roughly within the lateral or dorsolateral region. Axons from ventral medial MOB remain approximately in ventromedial region and those from middle medial MOB travel through either dorsolateral or ventromedial lot. Abbreviations: AOB, accessory olfactory bulb; AON, anterior olfactory nucleus; lot, lateral olfactory tract; lotd, dorsal limb of lot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3412993&req=5

Figure 5: Schematic of lateral and medial MOB projections to lot. Colors of injections in (A) and lot fibers in (B) are linked. Axons from dorsal (blue) or ventral (purple) lateral MOB travel directly toward the lot on the same side (A) and remain approximately in the dorsal and ventral lot, respectively (B). Fibers from dorsal medial MOB (orange) travel through the lotd, while those from ventral medial MOB (pink) take a ventrolateral route. Fibers originating from the middle medial MOB (green) travel either through the lotd or ventrolaterally toward lot. Within the lot, dorsal medial MOB fibers stay restricted roughly within the lateral or dorsolateral region. Axons from ventral medial MOB remain approximately in ventromedial region and those from middle medial MOB travel through either dorsolateral or ventromedial lot. Abbreviations: AOB, accessory olfactory bulb; AON, anterior olfactory nucleus; lot, lateral olfactory tract; lotd, dorsal limb of lot.

Mentions: Double coinjections were made into the (1) dorsal, (2) middle, or (3) ventral MOBmi along the dorsal-ventral axis and into medial or lateral regions along the medial-lateral axis (Figures 4A,C, 5A, 6A1,A2,B1,B2,C1).


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Schematic of lateral and medial MOB projections to lot. Colors of injections in (A) and lot fibers in (B) are linked. Axons from dorsal (blue) or ventral (purple) lateral MOB travel directly toward the lot on the same side (A) and remain approximately in the dorsal and ventral lot, respectively (B). Fibers from dorsal medial MOB (orange) travel through the lotd, while those from ventral medial MOB (pink) take a ventrolateral route. Fibers originating from the middle medial MOB (green) travel either through the lotd or ventrolaterally toward lot. Within the lot, dorsal medial MOB fibers stay restricted roughly within the lateral or dorsolateral region. Axons from ventral medial MOB remain approximately in ventromedial region and those from middle medial MOB travel through either dorsolateral or ventromedial lot. Abbreviations: AOB, accessory olfactory bulb; AON, anterior olfactory nucleus; lot, lateral olfactory tract; lotd, dorsal limb of lot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3412993&req=5

Figure 5: Schematic of lateral and medial MOB projections to lot. Colors of injections in (A) and lot fibers in (B) are linked. Axons from dorsal (blue) or ventral (purple) lateral MOB travel directly toward the lot on the same side (A) and remain approximately in the dorsal and ventral lot, respectively (B). Fibers from dorsal medial MOB (orange) travel through the lotd, while those from ventral medial MOB (pink) take a ventrolateral route. Fibers originating from the middle medial MOB (green) travel either through the lotd or ventrolaterally toward lot. Within the lot, dorsal medial MOB fibers stay restricted roughly within the lateral or dorsolateral region. Axons from ventral medial MOB remain approximately in ventromedial region and those from middle medial MOB travel through either dorsolateral or ventromedial lot. Abbreviations: AOB, accessory olfactory bulb; AON, anterior olfactory nucleus; lot, lateral olfactory tract; lotd, dorsal limb of lot.
Mentions: Double coinjections were made into the (1) dorsal, (2) middle, or (3) ventral MOBmi along the dorsal-ventral axis and into medial or lateral regions along the medial-lateral axis (Figures 4A,C, 5A, 6A1,A2,B1,B2,C1).

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus