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Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

Injections in the dorsal (A) and ventral (C) lateral MOB result in axons traveling directly toward the lot located on the same side. Within the lot, fibers from the dorsal lateral MOBmi travel predominantly in dorsal parts of the lot(B), while those from ventral lateral regions remain primarily in ventral lot(D) as they travel caudally toward their olfactory cortical destinations. Sections containing the lot are at the same level, approximately 2.745 mm anterior from bregma (B,D). Scale bar, 200 μm. Case numbers SW110608-05A (A,B), SW110607-03A (C,D).
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Figure 4: Injections in the dorsal (A) and ventral (C) lateral MOB result in axons traveling directly toward the lot located on the same side. Within the lot, fibers from the dorsal lateral MOBmi travel predominantly in dorsal parts of the lot(B), while those from ventral lateral regions remain primarily in ventral lot(D) as they travel caudally toward their olfactory cortical destinations. Sections containing the lot are at the same level, approximately 2.745 mm anterior from bregma (B,D). Scale bar, 200 μm. Case numbers SW110608-05A (A,B), SW110607-03A (C,D).

Mentions: Double coinjections were made into the (1) dorsal, (2) middle, or (3) ventral MOBmi along the dorsal-ventral axis and into medial or lateral regions along the medial-lateral axis (Figures 4A,C, 5A, 6A1,A2,B1,B2,C1).


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Injections in the dorsal (A) and ventral (C) lateral MOB result in axons traveling directly toward the lot located on the same side. Within the lot, fibers from the dorsal lateral MOBmi travel predominantly in dorsal parts of the lot(B), while those from ventral lateral regions remain primarily in ventral lot(D) as they travel caudally toward their olfactory cortical destinations. Sections containing the lot are at the same level, approximately 2.745 mm anterior from bregma (B,D). Scale bar, 200 μm. Case numbers SW110608-05A (A,B), SW110607-03A (C,D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3412993&req=5

Figure 4: Injections in the dorsal (A) and ventral (C) lateral MOB result in axons traveling directly toward the lot located on the same side. Within the lot, fibers from the dorsal lateral MOBmi travel predominantly in dorsal parts of the lot(B), while those from ventral lateral regions remain primarily in ventral lot(D) as they travel caudally toward their olfactory cortical destinations. Sections containing the lot are at the same level, approximately 2.745 mm anterior from bregma (B,D). Scale bar, 200 μm. Case numbers SW110608-05A (A,B), SW110607-03A (C,D).
Mentions: Double coinjections were made into the (1) dorsal, (2) middle, or (3) ventral MOBmi along the dorsal-ventral axis and into medial or lateral regions along the medial-lateral axis (Figures 4A,C, 5A, 6A1,A2,B1,B2,C1).

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus