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Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

Up to four cases can be viewed simultaneously in iConnectome for ease of comparing labeling from different cases (A). The four fluorescent tracers can be viewed per case: BDA in the red channel (upper left); PHAL in green (upper right, lower left); CTb in magenta (lower panels), and FG in yellow (upper right). Each tracer can be viewed either on their own bright-field Nissl background (B,C,G) or on their corresponding ARA level (D–F). Back-labeled FG neurons in MA (B) are magnified in (C) and are clearly registered onto the MA nucleus on ARA (D). ARA background shows CTb back-labeled neurons in the pyramidal layer of PIR (E), magnified in (F). Location of neurons in layer II of PIR clearly is also seen on bright-field Nissl (G). The thumbnail widget containing representative images of actual injection site size (H). Thumbnails containing interesting patterns of labeling from each tracer for each case can also be found in the widget.
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Figure 3: Up to four cases can be viewed simultaneously in iConnectome for ease of comparing labeling from different cases (A). The four fluorescent tracers can be viewed per case: BDA in the red channel (upper left); PHAL in green (upper right, lower left); CTb in magenta (lower panels), and FG in yellow (upper right). Each tracer can be viewed either on their own bright-field Nissl background (B,C,G) or on their corresponding ARA level (D–F). Back-labeled FG neurons in MA (B) are magnified in (C) and are clearly registered onto the MA nucleus on ARA (D). ARA background shows CTb back-labeled neurons in the pyramidal layer of PIR (E), magnified in (F). Location of neurons in layer II of PIR clearly is also seen on bright-field Nissl (G). The thumbnail widget containing representative images of actual injection site size (H). Thumbnails containing interesting patterns of labeling from each tracer for each case can also be found in the widget.

Mentions: The iConnectome is an interactive visualization tool for our whole-brain, high-resolution connectivity data. For each case, up to four fluorescent channels, each corresponding to a tracer, can be viewed and adjusted for brightness and contrast to reveal labeling of interest (Figure 3A). PHAL is represented in the green channel (Figure 3A, upper right and lower left panels), CTb in magenta (Figure 3A, lower panels), BDA in red (Figure 3A, upper left), and FG in yellow (Figure 3A, upper right). Up to four different cases (approximately 16 pathways) can be viewed simultaneously (Figure 3A). Windows can be synchronized such that any action performed in the master viewport is mirrored in the slave window(s) allowing comparison of labeling patterns from different cases.


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Up to four cases can be viewed simultaneously in iConnectome for ease of comparing labeling from different cases (A). The four fluorescent tracers can be viewed per case: BDA in the red channel (upper left); PHAL in green (upper right, lower left); CTb in magenta (lower panels), and FG in yellow (upper right). Each tracer can be viewed either on their own bright-field Nissl background (B,C,G) or on their corresponding ARA level (D–F). Back-labeled FG neurons in MA (B) are magnified in (C) and are clearly registered onto the MA nucleus on ARA (D). ARA background shows CTb back-labeled neurons in the pyramidal layer of PIR (E), magnified in (F). Location of neurons in layer II of PIR clearly is also seen on bright-field Nissl (G). The thumbnail widget containing representative images of actual injection site size (H). Thumbnails containing interesting patterns of labeling from each tracer for each case can also be found in the widget.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3412993&req=5

Figure 3: Up to four cases can be viewed simultaneously in iConnectome for ease of comparing labeling from different cases (A). The four fluorescent tracers can be viewed per case: BDA in the red channel (upper left); PHAL in green (upper right, lower left); CTb in magenta (lower panels), and FG in yellow (upper right). Each tracer can be viewed either on their own bright-field Nissl background (B,C,G) or on their corresponding ARA level (D–F). Back-labeled FG neurons in MA (B) are magnified in (C) and are clearly registered onto the MA nucleus on ARA (D). ARA background shows CTb back-labeled neurons in the pyramidal layer of PIR (E), magnified in (F). Location of neurons in layer II of PIR clearly is also seen on bright-field Nissl (G). The thumbnail widget containing representative images of actual injection site size (H). Thumbnails containing interesting patterns of labeling from each tracer for each case can also be found in the widget.
Mentions: The iConnectome is an interactive visualization tool for our whole-brain, high-resolution connectivity data. For each case, up to four fluorescent channels, each corresponding to a tracer, can be viewed and adjusted for brightness and contrast to reveal labeling of interest (Figure 3A). PHAL is represented in the green channel (Figure 3A, upper right and lower left panels), CTb in magenta (Figure 3A, lower panels), BDA in red (Figure 3A, upper left), and FG in yellow (Figure 3A, upper right). Up to four different cases (approximately 16 pathways) can be viewed simultaneously (Figure 3A). Windows can be synchronized such that any action performed in the master viewport is mirrored in the slave window(s) allowing comparison of labeling patterns from different cases.

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus