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Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus

Sagittal images of anterograde tracers Phaseolus vulgaris-leucoagglutinin (PHAL; green) and biotinylated dextran amine (BDA; red) injections in MOB (A, magnified in B). Fibers originating from MOB injections travel long distances across cortical olfactory areas (C). Magnified image of fibers in (D) shows BDA fibers in AON, PIR, and lot. Note the decrease in BDA axons across the PIR-OT junction (C,D). Whole-brain coronal sections of PHAL fibers from MOB traveling from rostral to caudal regions of the brain (E; −0.955 to −3.88 mm from bregma). Case numbers SW101215-02A (A); SW101213-01A (E). Scale bar, 1 mm (A,C); 200 μm (B,D). Abbreviations: MOB, main olfactory bulb; MOBgr, MOB granule layer; AOB, accessory olfactory bulb; PIR, piriform cortical area; OT, olfactory tubercle; AON, anterior olfactory nucleus; PHAL, Phaseolus vulgaris-leucoagglutinin; BDA, biotinylated dextran amine; NT, neurotrace blue.
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Figure 1: Sagittal images of anterograde tracers Phaseolus vulgaris-leucoagglutinin (PHAL; green) and biotinylated dextran amine (BDA; red) injections in MOB (A, magnified in B). Fibers originating from MOB injections travel long distances across cortical olfactory areas (C). Magnified image of fibers in (D) shows BDA fibers in AON, PIR, and lot. Note the decrease in BDA axons across the PIR-OT junction (C,D). Whole-brain coronal sections of PHAL fibers from MOB traveling from rostral to caudal regions of the brain (E; −0.955 to −3.88 mm from bregma). Case numbers SW101215-02A (A); SW101213-01A (E). Scale bar, 1 mm (A,C); 200 μm (B,D). Abbreviations: MOB, main olfactory bulb; MOBgr, MOB granule layer; AOB, accessory olfactory bulb; PIR, piriform cortical area; OT, olfactory tubercle; AON, anterior olfactory nucleus; PHAL, Phaseolus vulgaris-leucoagglutinin; BDA, biotinylated dextran amine; NT, neurotrace blue.

Mentions: In his pioneering work, Ramon y Cajal (1904) elegantly illustrated the cytoarchitecture of the main olfactory bulb (MOB) and its general pathways to cortical destinations using the Golgi stain. Since then, olfactory pathways have been thoroughly examined using more advanced techniques, ranging from circuit tracers to cell-type specific viral tracers (Cragg, 1961; Powell et al., 1965; Price, 1973; Scalia and Winans, 1975; Shipley and Adamek, 1984; Pro-Sistiaga et al., 2007; Yan et al., 2008; Nagayama et al., 2010; Miyamichi et al., 2011; Sosulski et al., 2011; for reviews see Haberly, 2001; Friedrich, 2011). Technological advancements also have improved presentation of neuroconnectivity data. High cost and space limitations typically allow publication of figures depicting connectivity that is of most interest, precluding presentation of the majority of the data. Unfortunately, this provides only a partial view of the whole picture. Presently, advanced imaging equipment and computer technology have revolutionized neuroanatomy such that whole-brain high-resolution images can be acquired and made publicly accessible for world-wide use (Dong, 2007; Jones et al., 2011). At the forefront of this new neuroanatomy era is the iConnecome, where systematically accumulated multi-fluorescent connectivity data can be accessed (www.MouseConnectome.org). Whole-brain coronal sections starting from rostral regions of the olfactory bulb and extending to the caudal regions of the hindbrain are available for each case. For example, olfactory cortical projections from the MOB and accessory olfactory bulbs (AOBs) extend long distances reaching the caudal ends of the entorhinal cortical area (ENT) (Figures 1A–E). Coronal sections with all of the labeling from each injection site are available in iConnectome and provide a comprehensive view of connections associated with each injected structure (Figure 1E).


Comprehensive connectivity of the mouse main olfactory bulb: analysis and online digital atlas.

Hintiryan H, Gou L, Zingg B, Yamashita S, Lyden HM, Song MY, Grewal AK, Zhang X, Toga AW, Dong HW - Front Neuroanat (2012)

Sagittal images of anterograde tracers Phaseolus vulgaris-leucoagglutinin (PHAL; green) and biotinylated dextran amine (BDA; red) injections in MOB (A, magnified in B). Fibers originating from MOB injections travel long distances across cortical olfactory areas (C). Magnified image of fibers in (D) shows BDA fibers in AON, PIR, and lot. Note the decrease in BDA axons across the PIR-OT junction (C,D). Whole-brain coronal sections of PHAL fibers from MOB traveling from rostral to caudal regions of the brain (E; −0.955 to −3.88 mm from bregma). Case numbers SW101215-02A (A); SW101213-01A (E). Scale bar, 1 mm (A,C); 200 μm (B,D). Abbreviations: MOB, main olfactory bulb; MOBgr, MOB granule layer; AOB, accessory olfactory bulb; PIR, piriform cortical area; OT, olfactory tubercle; AON, anterior olfactory nucleus; PHAL, Phaseolus vulgaris-leucoagglutinin; BDA, biotinylated dextran amine; NT, neurotrace blue.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3412993&req=5

Figure 1: Sagittal images of anterograde tracers Phaseolus vulgaris-leucoagglutinin (PHAL; green) and biotinylated dextran amine (BDA; red) injections in MOB (A, magnified in B). Fibers originating from MOB injections travel long distances across cortical olfactory areas (C). Magnified image of fibers in (D) shows BDA fibers in AON, PIR, and lot. Note the decrease in BDA axons across the PIR-OT junction (C,D). Whole-brain coronal sections of PHAL fibers from MOB traveling from rostral to caudal regions of the brain (E; −0.955 to −3.88 mm from bregma). Case numbers SW101215-02A (A); SW101213-01A (E). Scale bar, 1 mm (A,C); 200 μm (B,D). Abbreviations: MOB, main olfactory bulb; MOBgr, MOB granule layer; AOB, accessory olfactory bulb; PIR, piriform cortical area; OT, olfactory tubercle; AON, anterior olfactory nucleus; PHAL, Phaseolus vulgaris-leucoagglutinin; BDA, biotinylated dextran amine; NT, neurotrace blue.
Mentions: In his pioneering work, Ramon y Cajal (1904) elegantly illustrated the cytoarchitecture of the main olfactory bulb (MOB) and its general pathways to cortical destinations using the Golgi stain. Since then, olfactory pathways have been thoroughly examined using more advanced techniques, ranging from circuit tracers to cell-type specific viral tracers (Cragg, 1961; Powell et al., 1965; Price, 1973; Scalia and Winans, 1975; Shipley and Adamek, 1984; Pro-Sistiaga et al., 2007; Yan et al., 2008; Nagayama et al., 2010; Miyamichi et al., 2011; Sosulski et al., 2011; for reviews see Haberly, 2001; Friedrich, 2011). Technological advancements also have improved presentation of neuroconnectivity data. High cost and space limitations typically allow publication of figures depicting connectivity that is of most interest, precluding presentation of the majority of the data. Unfortunately, this provides only a partial view of the whole picture. Presently, advanced imaging equipment and computer technology have revolutionized neuroanatomy such that whole-brain high-resolution images can be acquired and made publicly accessible for world-wide use (Dong, 2007; Jones et al., 2011). At the forefront of this new neuroanatomy era is the iConnecome, where systematically accumulated multi-fluorescent connectivity data can be accessed (www.MouseConnectome.org). Whole-brain coronal sections starting from rostral regions of the olfactory bulb and extending to the caudal regions of the hindbrain are available for each case. For example, olfactory cortical projections from the MOB and accessory olfactory bulbs (AOBs) extend long distances reaching the caudal ends of the entorhinal cortical area (ENT) (Figures 1A–E). Coronal sections with all of the labeling from each injection site are available in iConnectome and provide a comprehensive view of connections associated with each injected structure (Figure 1E).

Bottom Line: To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org).Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available.Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuro Imaging, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles Los Angeles, CA, USA.

ABSTRACT
We introduce the first open resource for mouse olfactory connectivity data produced as part of the Mouse Connectome Project (MCP) at UCLA. The MCP aims to assemble a whole-brain connectivity atlas for the C57Bl/6J mouse using a double coinjection tracing method. Each coinjection consists of one anterograde and one retrograde tracer, which affords the advantage of simultaneously identifying efferent and afferent pathways and directly identifying reciprocal connectivity of injection sites. The systematic application of double coinjections potentially reveals interaction stations between injections and allows for the study of connectivity at the network level. To facilitate use of the data, raw images are made publicly accessible through our online interactive visualization tool, the iConnectome, where users can view and annotate the high-resolution, multi-fluorescent connectivity data (www.MouseConnectome.org). Systematic double coinjections were made into different regions of the main olfactory bulb (MOB) and data from 18 MOB cases (~72 pathways; 36 efferent/36 afferent) currently are available to view in iConnectome within their corresponding atlas level and their own bright-field cytoarchitectural background. Additional MOB injections and injections of the accessory olfactory bulb (AOB), anterior olfactory nucleus (AON), and other olfactory cortical areas gradually will be made available. Analysis of connections from different regions of the MOB revealed a novel, topographically arranged MOB projection roadmap, demonstrated disparate MOB connectivity with anterior versus posterior piriform cortical area (PIR), and exposed some novel aspects of well-established cortical olfactory projections.

No MeSH data available.


Related in: MedlinePlus