Limits...
Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH

Related in: MedlinePlus

Correlations of sputum galectin-10 amount versus: (a) % sputum eosinophil count (rs = 0.695, p<0.001), (b): eosinophil concentration (rs = 0.786, p<0.001), and (c): % sputum neutrophil count (rs = 0.243, p = 0.141).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g004: Correlations of sputum galectin-10 amount versus: (a) % sputum eosinophil count (rs = 0.695, p<0.001), (b): eosinophil concentration (rs = 0.786, p<0.001), and (c): % sputum neutrophil count (rs = 0.243, p = 0.141).

Mentions: The sputum galectin-10 levels showed a highly significant correlation with sputum eosinophil counts (rs = 0.695, p<0.001) (Figure 4a). The correlation was stronger with sputum eosinophil concentrations (rs = 0.786, p<0.001) (Figure 4b). These correlations remained high and significant whether sputum samples were analyzed individually or results were averaged for each person (data not shown). When only the asthmatic samples were selected, the correlation of galectin-10 levels with sputum eosinophil counts and concentrations was even stronger, rs = 0.722 (p<0.001) and rs = 0.800 (p<0.001) respectively.


Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Correlations of sputum galectin-10 amount versus: (a) % sputum eosinophil count (rs = 0.695, p<0.001), (b): eosinophil concentration (rs = 0.786, p<0.001), and (c): % sputum neutrophil count (rs = 0.243, p = 0.141).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g004: Correlations of sputum galectin-10 amount versus: (a) % sputum eosinophil count (rs = 0.695, p<0.001), (b): eosinophil concentration (rs = 0.786, p<0.001), and (c): % sputum neutrophil count (rs = 0.243, p = 0.141).
Mentions: The sputum galectin-10 levels showed a highly significant correlation with sputum eosinophil counts (rs = 0.695, p<0.001) (Figure 4a). The correlation was stronger with sputum eosinophil concentrations (rs = 0.786, p<0.001) (Figure 4b). These correlations remained high and significant whether sputum samples were analyzed individually or results were averaged for each person (data not shown). When only the asthmatic samples were selected, the correlation of galectin-10 levels with sputum eosinophil counts and concentrations was even stronger, rs = 0.722 (p<0.001) and rs = 0.800 (p<0.001) respectively.

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH
Related in: MedlinePlus