Limits...
Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH

Related in: MedlinePlus

Cytospins of sputum samples from an asthma with ABPA patient which contained bi-pyramidal Charcot-Leyden crystals (arrows).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g003: Cytospins of sputum samples from an asthma with ABPA patient which contained bi-pyramidal Charcot-Leyden crystals (arrows).

Mentions: During microscopic examination of sputum cytospins, four samples with a high percentage of eosinophils (10.5%) were identified as containing the characteristic bi-pyramidal, hexagonal Charcot-Leyden crystals (Figure 3).


Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Cytospins of sputum samples from an asthma with ABPA patient which contained bi-pyramidal Charcot-Leyden crystals (arrows).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g003: Cytospins of sputum samples from an asthma with ABPA patient which contained bi-pyramidal Charcot-Leyden crystals (arrows).
Mentions: During microscopic examination of sputum cytospins, four samples with a high percentage of eosinophils (10.5%) were identified as containing the characteristic bi-pyramidal, hexagonal Charcot-Leyden crystals (Figure 3).

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH
Related in: MedlinePlus