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Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

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Related in: MedlinePlus

Sputum eosinophil (a&b) and neutrophil (c) levels across the main diagnosis groups.* = significant difference with the healthy control group (p<0.01).
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pone-0042549-g002: Sputum eosinophil (a&b) and neutrophil (c) levels across the main diagnosis groups.* = significant difference with the healthy control group (p<0.01).

Mentions: All differential cell counts of the sputum samples are shown in Table 2 and Figure 2. Out of 38 analyzable samples, 11 had >3% eosinophils. The majority of the positive counts came from the asthma with ABPA group with 7 positive results and the remaining 4 from the asthma without ABPA group. By this criterion, there were no positive sputum eosinophil counts among the healthy control, bronchiectasis and interstitial pulmonary fibrosis participants.


Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Sputum eosinophil (a&b) and neutrophil (c) levels across the main diagnosis groups.* = significant difference with the healthy control group (p<0.01).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g002: Sputum eosinophil (a&b) and neutrophil (c) levels across the main diagnosis groups.* = significant difference with the healthy control group (p<0.01).
Mentions: All differential cell counts of the sputum samples are shown in Table 2 and Figure 2. Out of 38 analyzable samples, 11 had >3% eosinophils. The majority of the positive counts came from the asthma with ABPA group with 7 positive results and the remaining 4 from the asthma without ABPA group. By this criterion, there were no positive sputum eosinophil counts among the healthy control, bronchiectasis and interstitial pulmonary fibrosis participants.

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH
Related in: MedlinePlus