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Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

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Related in: MedlinePlus

A Western blot demonstrating the positive control sample diluted to varying concentrations to create a calibration curve.The numbers at the bottom of the bands represent the degree of dilution (initial sample volume: total diluted volume).
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pone-0042549-g001: A Western blot demonstrating the positive control sample diluted to varying concentrations to create a calibration curve.The numbers at the bottom of the bands represent the degree of dilution (initial sample volume: total diluted volume).

Mentions: To semi-quantify the samples, a sputum sample from a participant with high levels of galectin-10 was chosen as a positive control in all western blot assays (Figure 1). Different sample bands' optical density were calculated as a percentage of the positive control band multiplied by a factor of 100 and named the Relative Galectin-10 Unit (RGU).


Galectin-10, a potential biomarker of eosinophilic airway inflammation.

Chua JC, Douglass JA, Gillman A, O'Hehir RE, Meeusen EN - PLoS ONE (2012)

A Western blot demonstrating the positive control sample diluted to varying concentrations to create a calibration curve.The numbers at the bottom of the bands represent the degree of dilution (initial sample volume: total diluted volume).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3412795&req=5

pone-0042549-g001: A Western blot demonstrating the positive control sample diluted to varying concentrations to create a calibration curve.The numbers at the bottom of the bands represent the degree of dilution (initial sample volume: total diluted volume).
Mentions: To semi-quantify the samples, a sputum sample from a participant with high levels of galectin-10 was chosen as a positive control in all western blot assays (Figure 1). Different sample bands' optical density were calculated as a percentage of the positive control band multiplied by a factor of 100 and named the Relative Galectin-10 Unit (RGU).

Bottom Line: Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker.Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots.The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia.

ABSTRACT
Measurement of eosinophilic airway inflammation can assist in the diagnosis of allergic asthma and in the management of exacerbations, however its clinical implementation remains difficult. Galectin-10 has been associated with eosinophilic inflammation and has the potential to be used as a surrogate biomarker. This study aimed to assess the relationship between galectin-10 in sputum with sputum eosinophil counts, the current gold standard of eosinophil inflammation in the lung. Thirty-eight sputum samples were processed for both eosinophil counts by cytospins and semi-quantitative measurements of galectin-10 by western blots. A strong association was observed between galectin-10 levels in sputum and sputum eosinophil measurements, and they accurately determined sputum eosinophilia. The results support the potential for galectin-10 to be used as a surrogate biomarker of eosinophilic airway inflammation.

Show MeSH
Related in: MedlinePlus