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Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus.

Chang SJ, Chang YC, Lu KZ, Tsou YY, Lin CW - Evid Based Complement Alternat Med (2012)

Bottom Line: Time-of-addition experiments proved the extract, indigo, and indirubin with potent antiviral effect by pretreatment (before infection) or simultaneous treatment (during infection), but not posttreatment (after entry).In particular, indirubin had strong protective ability in a mouse model with lethal JEV challenge.The study could yield anti-JEV agents.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, China Medical University, Taichung 404, Taiwan.

ABSTRACT
Isatis indigotica is widely used in Chinese Traditional Medicine for clinical treatment of virus infection, tumor, and inflammation, yet its antiviral activities remain unclear. This study probed antiviral activity of I. indigotica extract and its marker compounds against Japanese encephalitis virus (JEV). I. indigotica methanol extract, indigo, and indirubin proved less cytotoxic than other components, showing inhibitory effect (concentration-dependent) on JEV replication in vitro. Time-of-addition experiments proved the extract, indigo, and indirubin with potent antiviral effect by pretreatment (before infection) or simultaneous treatment (during infection), but not posttreatment (after entry). Antiviral action of these agents showed correlation with blocking virus attachment and exhibited potent virucidal activity. In particular, indirubin had strong protective ability in a mouse model with lethal JEV challenge. The study could yield anti-JEV agents.

No MeSH data available.


Related in: MedlinePlus

Virucidal activities of I. indigotica and its major components. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV. Each virus/compound mixture was incubated at 4°C for 1 h, then added onto the BHK-21 cell monolayer at 37°C for another 1 h. The virus/compound mixture was removed from 6-well plates and cell monolayer washed with PBS. Residual infectivity was performed as described in plaque assay.
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fig4: Virucidal activities of I. indigotica and its major components. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV. Each virus/compound mixture was incubated at 4°C for 1 h, then added onto the BHK-21 cell monolayer at 37°C for another 1 h. The virus/compound mixture was removed from 6-well plates and cell monolayer washed with PBS. Residual infectivity was performed as described in plaque assay.

Mentions: To ascertain whether I. indigotica has a virucidal action by directly interfering with virus particles, JEV was preincubated with both indigo and indirubin at 4°C for 1 h, and residual infectivity tested by plaque assay (Figure 4 and Table 2). Both indigo and indirubin exhibited concentration-dependent virucidal activity as well as significant inhibitory effect on residual infectivity compared to controls. Virucidal IC50 values against JEV were 65.79 μg/mL of ethyl acetate extract, 22.17 μg/mL of methanol extract, 3.03 μg/mL of indigo, and 0.47 μg/mL of indirubin. Moreover, virucidal IC50 values were below pre-, simultaneous, and posttreatment as well virus attachment assay (Table 2), revealing that I. indigotica directly inactivated JEV particles, exhibiting a potently virucidal action.


Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus.

Chang SJ, Chang YC, Lu KZ, Tsou YY, Lin CW - Evid Based Complement Alternat Med (2012)

Virucidal activities of I. indigotica and its major components. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV. Each virus/compound mixture was incubated at 4°C for 1 h, then added onto the BHK-21 cell monolayer at 37°C for another 1 h. The virus/compound mixture was removed from 6-well plates and cell monolayer washed with PBS. Residual infectivity was performed as described in plaque assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3405817&req=5

fig4: Virucidal activities of I. indigotica and its major components. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV. Each virus/compound mixture was incubated at 4°C for 1 h, then added onto the BHK-21 cell monolayer at 37°C for another 1 h. The virus/compound mixture was removed from 6-well plates and cell monolayer washed with PBS. Residual infectivity was performed as described in plaque assay.
Mentions: To ascertain whether I. indigotica has a virucidal action by directly interfering with virus particles, JEV was preincubated with both indigo and indirubin at 4°C for 1 h, and residual infectivity tested by plaque assay (Figure 4 and Table 2). Both indigo and indirubin exhibited concentration-dependent virucidal activity as well as significant inhibitory effect on residual infectivity compared to controls. Virucidal IC50 values against JEV were 65.79 μg/mL of ethyl acetate extract, 22.17 μg/mL of methanol extract, 3.03 μg/mL of indigo, and 0.47 μg/mL of indirubin. Moreover, virucidal IC50 values were below pre-, simultaneous, and posttreatment as well virus attachment assay (Table 2), revealing that I. indigotica directly inactivated JEV particles, exhibiting a potently virucidal action.

Bottom Line: Time-of-addition experiments proved the extract, indigo, and indirubin with potent antiviral effect by pretreatment (before infection) or simultaneous treatment (during infection), but not posttreatment (after entry).In particular, indirubin had strong protective ability in a mouse model with lethal JEV challenge.The study could yield anti-JEV agents.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, China Medical University, Taichung 404, Taiwan.

ABSTRACT
Isatis indigotica is widely used in Chinese Traditional Medicine for clinical treatment of virus infection, tumor, and inflammation, yet its antiviral activities remain unclear. This study probed antiviral activity of I. indigotica extract and its marker compounds against Japanese encephalitis virus (JEV). I. indigotica methanol extract, indigo, and indirubin proved less cytotoxic than other components, showing inhibitory effect (concentration-dependent) on JEV replication in vitro. Time-of-addition experiments proved the extract, indigo, and indirubin with potent antiviral effect by pretreatment (before infection) or simultaneous treatment (during infection), but not posttreatment (after entry). Antiviral action of these agents showed correlation with blocking virus attachment and exhibited potent virucidal activity. In particular, indirubin had strong protective ability in a mouse model with lethal JEV challenge. The study could yield anti-JEV agents.

No MeSH data available.


Related in: MedlinePlus