A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus.
Bottom Line: Detection limits of 9.0×10(1) and 6.0×10(2)copies/mL were observed for RSV A and B, respectively.RSV A was detected in 53/84 (63%) cases, and 31/84 (37%) were positive for RSV B.This novel method offers a rapid, quantitative, highly specific and sensitive approach to laboratory diagnosis of RSV.
Affiliation: Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Viet Nam. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: The viral load of RSV detected in 84 samples ranged from 4.0 × 103 to 109 copies/mL (median value 2.9 × 106; interquartile range (IQR) 2.7 × 105 to 4.2 × 107). Median (IQR) of viral load in 64 positive samples by both the RSV LNA assay and the Seeplex kit was 8.0 × 106 copies/mL (2.0 × 105 to 5.0 × 107). In the 20 samples positive by RSV LNA assay alone, median (IQR) of viral load was 3.5 × 104 copies/mL (1.7 × 104 to 4.3 × 105). There was a significant difference observed in viral load between RSV positive samples diagnosed by both assays and RSV positive samples by only RSV LNA assay (Mann–Whitney U test, p = 0.001) (Fig. 1).
Affiliation: Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Viet Nam. firstname.lastname@example.org