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A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus.

Do LA, van Doorn HR, Bryant JE, Nghiem MN, Nguyen Van VC, Vo CK, Nguyen MD, Tran TH, Farrar J, de Jong MD - J. Virol. Methods (2011)

Bottom Line: Detection limits of 9.0×10(1) and 6.0×10(2)copies/mL were observed for RSV A and B, respectively.RSV A was detected in 53/84 (63%) cases, and 31/84 (37%) were positive for RSV B.This novel method offers a rapid, quantitative, highly specific and sensitive approach to laboratory diagnosis of RSV.

View Article: PubMed Central - PubMed

Affiliation: Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Viet Nam. hadla@oucru.org

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Viral load detected in RSV positive samples diagnosed by both assays and RSV positive samples by only LNA assay.
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fig0005: Viral load detected in RSV positive samples diagnosed by both assays and RSV positive samples by only LNA assay.

Mentions: The viral load of RSV detected in 84 samples ranged from 4.0 × 103 to 109 copies/mL (median value 2.9 × 106; interquartile range (IQR) 2.7 × 105 to 4.2 × 107). Median (IQR) of viral load in 64 positive samples by both the RSV LNA assay and the Seeplex kit was 8.0 × 106 copies/mL (2.0 × 105 to 5.0 × 107). In the 20 samples positive by RSV LNA assay alone, median (IQR) of viral load was 3.5 × 104 copies/mL (1.7 × 104 to 4.3 × 105). There was a significant difference observed in viral load between RSV positive samples diagnosed by both assays and RSV positive samples by only RSV LNA assay (Mann–Whitney U test, p = 0.001) (Fig. 1).


A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus.

Do LA, van Doorn HR, Bryant JE, Nghiem MN, Nguyen Van VC, Vo CK, Nguyen MD, Tran TH, Farrar J, de Jong MD - J. Virol. Methods (2011)

Viral load detected in RSV positive samples diagnosed by both assays and RSV positive samples by only LNA assay.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3405522&req=5

fig0005: Viral load detected in RSV positive samples diagnosed by both assays and RSV positive samples by only LNA assay.
Mentions: The viral load of RSV detected in 84 samples ranged from 4.0 × 103 to 109 copies/mL (median value 2.9 × 106; interquartile range (IQR) 2.7 × 105 to 4.2 × 107). Median (IQR) of viral load in 64 positive samples by both the RSV LNA assay and the Seeplex kit was 8.0 × 106 copies/mL (2.0 × 105 to 5.0 × 107). In the 20 samples positive by RSV LNA assay alone, median (IQR) of viral load was 3.5 × 104 copies/mL (1.7 × 104 to 4.3 × 105). There was a significant difference observed in viral load between RSV positive samples diagnosed by both assays and RSV positive samples by only RSV LNA assay (Mann–Whitney U test, p = 0.001) (Fig. 1).

Bottom Line: Detection limits of 9.0×10(1) and 6.0×10(2)copies/mL were observed for RSV A and B, respectively.RSV A was detected in 53/84 (63%) cases, and 31/84 (37%) were positive for RSV B.This novel method offers a rapid, quantitative, highly specific and sensitive approach to laboratory diagnosis of RSV.

View Article: PubMed Central - PubMed

Affiliation: Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Viet Nam. hadla@oucru.org

Show MeSH
Related in: MedlinePlus