Visualisation of J-type counter-current chromatography: a route to understand hydrodynamic phase distribution and retention.
Bottom Line: The experimental results thus obtained were used to examine the effects of the liquid-solid friction force, tangential centrifugal force, and physical properties of the two-phase system on hydrodynamic phase behaviour.This work thus has extended or modified the well-established rule-of-thumb for operating J-type CCC devices and our conclusions can accommodate virtually all the anomalies concerning both hydrophobic and hydrophilic phase systems.Revised recommendations to end users of this technology could thus be derived out of the essence of the present work presumably following further experimental validation and a consensus in the CCC R&D and manufacturing circle.
Affiliation: State Key Laboratory of Bioreactor Engineering, & The College of Biotechnology, East China University of Science & Technology, Shanghai 200237, China. email@example.comShow MeSH
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Mentions: The phase replacement dynamics for L-O-T, U-I-H, U-O-T, L-O-H, U-O-H and U-I-T flow modes were briefed in Fig. 6. At the beginning of a dynamic process, the 2-D spiral column was invariably filled with stationary phase. Observation was made using digital images which were taken under the stroboscopic illumination at a constant time interval. More imaging results in JPEG format were given in Supplementary materials 1 through 8 (i.e. S1–S8) to the present paper (including the results for L-I-H and L-I-T flow modes). It is hoped that these JPEG-format results will withstand further analyses and/or comparisons.
Affiliation: State Key Laboratory of Bioreactor Engineering, & The College of Biotechnology, East China University of Science & Technology, Shanghai 200237, China. firstname.lastname@example.org