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Visualisation of J-type counter-current chromatography: a route to understand hydrodynamic phase distribution and retention.

Guan YH, van den Heuvel RN, Zhuang YP - J Chromatogr A (2012)

Bottom Line: The experimental results thus obtained were used to examine the effects of the liquid-solid friction force, tangential centrifugal force, and physical properties of the two-phase system on hydrodynamic phase behaviour.This work thus has extended or modified the well-established rule-of-thumb for operating J-type CCC devices and our conclusions can accommodate virtually all the anomalies concerning both hydrophobic and hydrophilic phase systems.Revised recommendations to end users of this technology could thus be derived out of the essence of the present work presumably following further experimental validation and a consensus in the CCC R&D and manufacturing circle.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioreactor Engineering, & The College of Biotechnology, East China University of Science & Technology, Shanghai 200237, China. y.h.guan@ecust.edu.cn

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Snapshots at different times of the transitional periods with 4 ml/min mobile phase flow rate for 6 flow modes where at least majority of the intended stationary phase was replaced by the mobile phase in the 2-D spiral column. At the beginning of a transitional period, the 2-D spiral column was filled with the intended stationary phase. Rotation of the J-type CCC centrifuge at 800 rpm was shortly followed by initiation of the mobile phase flow using an external HPLC pump. Results are more discernible in the PDF colour version. All the detailed results are shown respectively in S2, S3, S4(part1), S4(part2), S5, S7 and S8 (Supplementary material).
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fig0030: Snapshots at different times of the transitional periods with 4 ml/min mobile phase flow rate for 6 flow modes where at least majority of the intended stationary phase was replaced by the mobile phase in the 2-D spiral column. At the beginning of a transitional period, the 2-D spiral column was filled with the intended stationary phase. Rotation of the J-type CCC centrifuge at 800 rpm was shortly followed by initiation of the mobile phase flow using an external HPLC pump. Results are more discernible in the PDF colour version. All the detailed results are shown respectively in S2, S3, S4(part1), S4(part2), S5, S7 and S8 (Supplementary material).

Mentions: The phase replacement dynamics for L-O-T, U-I-H, U-O-T, L-O-H, U-O-H and U-I-T flow modes were briefed in Fig. 6. At the beginning of a dynamic process, the 2-D spiral column was invariably filled with stationary phase. Observation was made using digital images which were taken under the stroboscopic illumination at a constant time interval. More imaging results in JPEG format were given in Supplementary materials 1 through 8 (i.e. S1–S8) to the present paper (including the results for L-I-H and L-I-T flow modes). It is hoped that these JPEG-format results will withstand further analyses and/or comparisons.


Visualisation of J-type counter-current chromatography: a route to understand hydrodynamic phase distribution and retention.

Guan YH, van den Heuvel RN, Zhuang YP - J Chromatogr A (2012)

Snapshots at different times of the transitional periods with 4 ml/min mobile phase flow rate for 6 flow modes where at least majority of the intended stationary phase was replaced by the mobile phase in the 2-D spiral column. At the beginning of a transitional period, the 2-D spiral column was filled with the intended stationary phase. Rotation of the J-type CCC centrifuge at 800 rpm was shortly followed by initiation of the mobile phase flow using an external HPLC pump. Results are more discernible in the PDF colour version. All the detailed results are shown respectively in S2, S3, S4(part1), S4(part2), S5, S7 and S8 (Supplementary material).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3405518&req=5

fig0030: Snapshots at different times of the transitional periods with 4 ml/min mobile phase flow rate for 6 flow modes where at least majority of the intended stationary phase was replaced by the mobile phase in the 2-D spiral column. At the beginning of a transitional period, the 2-D spiral column was filled with the intended stationary phase. Rotation of the J-type CCC centrifuge at 800 rpm was shortly followed by initiation of the mobile phase flow using an external HPLC pump. Results are more discernible in the PDF colour version. All the detailed results are shown respectively in S2, S3, S4(part1), S4(part2), S5, S7 and S8 (Supplementary material).
Mentions: The phase replacement dynamics for L-O-T, U-I-H, U-O-T, L-O-H, U-O-H and U-I-T flow modes were briefed in Fig. 6. At the beginning of a dynamic process, the 2-D spiral column was invariably filled with stationary phase. Observation was made using digital images which were taken under the stroboscopic illumination at a constant time interval. More imaging results in JPEG format were given in Supplementary materials 1 through 8 (i.e. S1–S8) to the present paper (including the results for L-I-H and L-I-T flow modes). It is hoped that these JPEG-format results will withstand further analyses and/or comparisons.

Bottom Line: The experimental results thus obtained were used to examine the effects of the liquid-solid friction force, tangential centrifugal force, and physical properties of the two-phase system on hydrodynamic phase behaviour.This work thus has extended or modified the well-established rule-of-thumb for operating J-type CCC devices and our conclusions can accommodate virtually all the anomalies concerning both hydrophobic and hydrophilic phase systems.Revised recommendations to end users of this technology could thus be derived out of the essence of the present work presumably following further experimental validation and a consensus in the CCC R&D and manufacturing circle.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Bioreactor Engineering, & The College of Biotechnology, East China University of Science & Technology, Shanghai 200237, China. y.h.guan@ecust.edu.cn

Show MeSH
Related in: MedlinePlus