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Antimicrobial activity and genetic profile of Enteroccoci isolated from hoopoes uropygial gland.

Ruiz-Rodríguez M, Valdivia E, Martín-Vivaldi M, Martín-Platero AM, Martínez-Bueno M, Méndez M, Peralta-Sánchez JM, Soler JJ - PLoS ONE (2012)

Bottom Line: If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one.Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria).This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

View Article: PubMed Central - PubMed

Affiliation: Departamento Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas (CSIC), Almería, Spain. magda@eeza.csic.es

ABSTRACT
Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria). We explored variability in genetic profile (through RAPD-PCR analyses) and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains) of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

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Related in: MedlinePlus

Intensity of antimicrobial activity.Variation in the inhibitory intensity against each indicator strain by symbiotic bacteria isolated from the uropygial gland secretion of hoopoes. (SA: S. aureus; ML: M. luteus; LM: L. monocytogenes; LL: L. lactis; LI: L. innocua; BL: B. licheniformis; EF: E. faecium; S47: E. faecalis). Intensity of antagonistic activity: 0 (no halo), 1 (ring width <1 mm), 2 (ring width = 1–2 mm), 3 (ring width = 3–4 mm), and 4 (ring width >4 mm.).
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pone-0041843-g002: Intensity of antimicrobial activity.Variation in the inhibitory intensity against each indicator strain by symbiotic bacteria isolated from the uropygial gland secretion of hoopoes. (SA: S. aureus; ML: M. luteus; LM: L. monocytogenes; LL: L. lactis; LI: L. innocua; BL: B. licheniformis; EF: E. faecium; S47: E. faecalis). Intensity of antagonistic activity: 0 (no halo), 1 (ring width <1 mm), 2 (ring width = 1–2 mm), 3 (ring width = 3–4 mm), and 4 (ring width >4 mm.).

Mentions: Antagonistic activity was found in 202 colonies (70.73%) isolated from hoopoe’s uropygial glands. Those colonies belonged to 55 individuals (95%) from all the 24 nests (100%). Only 3 individual nestlings growing in 2 different nests didn’t show any antagonistic activity against the indicator strains used. In the resulting 57 RAPD groups, antimicrobial activity was detected for 45 of them (78.94%). The number of indicator strains that an isolate was able to inhibit varied from 0 to 8 (Figure 1) with mode and average values of 2 and 2.42 respectively. Moreover, antimicrobial activity and its intensity against different indicators were also very variable (Table 1, Figure 2). The indicator species against which the symbiotic bacteria of hoopoes showed the greatest and the less activity were respectively L. lactis and S. aureus.


Antimicrobial activity and genetic profile of Enteroccoci isolated from hoopoes uropygial gland.

Ruiz-Rodríguez M, Valdivia E, Martín-Vivaldi M, Martín-Platero AM, Martínez-Bueno M, Méndez M, Peralta-Sánchez JM, Soler JJ - PLoS ONE (2012)

Intensity of antimicrobial activity.Variation in the inhibitory intensity against each indicator strain by symbiotic bacteria isolated from the uropygial gland secretion of hoopoes. (SA: S. aureus; ML: M. luteus; LM: L. monocytogenes; LL: L. lactis; LI: L. innocua; BL: B. licheniformis; EF: E. faecium; S47: E. faecalis). Intensity of antagonistic activity: 0 (no halo), 1 (ring width <1 mm), 2 (ring width = 1–2 mm), 3 (ring width = 3–4 mm), and 4 (ring width >4 mm.).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3404078&req=5

pone-0041843-g002: Intensity of antimicrobial activity.Variation in the inhibitory intensity against each indicator strain by symbiotic bacteria isolated from the uropygial gland secretion of hoopoes. (SA: S. aureus; ML: M. luteus; LM: L. monocytogenes; LL: L. lactis; LI: L. innocua; BL: B. licheniformis; EF: E. faecium; S47: E. faecalis). Intensity of antagonistic activity: 0 (no halo), 1 (ring width <1 mm), 2 (ring width = 1–2 mm), 3 (ring width = 3–4 mm), and 4 (ring width >4 mm.).
Mentions: Antagonistic activity was found in 202 colonies (70.73%) isolated from hoopoe’s uropygial glands. Those colonies belonged to 55 individuals (95%) from all the 24 nests (100%). Only 3 individual nestlings growing in 2 different nests didn’t show any antagonistic activity against the indicator strains used. In the resulting 57 RAPD groups, antimicrobial activity was detected for 45 of them (78.94%). The number of indicator strains that an isolate was able to inhibit varied from 0 to 8 (Figure 1) with mode and average values of 2 and 2.42 respectively. Moreover, antimicrobial activity and its intensity against different indicators were also very variable (Table 1, Figure 2). The indicator species against which the symbiotic bacteria of hoopoes showed the greatest and the less activity were respectively L. lactis and S. aureus.

Bottom Line: If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one.Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria).This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

View Article: PubMed Central - PubMed

Affiliation: Departamento Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas (CSIC), Almería, Spain. magda@eeza.csic.es

ABSTRACT
Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria). We explored variability in genetic profile (through RAPD-PCR analyses) and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains) of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

Show MeSH
Related in: MedlinePlus