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Hypoglycemic and antilipidemic properties of kombucha tea in alloxan-induced diabetic rats.

Aloulou A, Hamden K, Elloumi D, Ali MB, Hargafi K, Jaouadi B, Ayadi F, Elfeki A, Ammar E - BMC Complement Altern Med (2012)

Bottom Line: The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents.The findings revealed that, compared to black tea, kombucha tea was a better inhibitor of α-amylase and lipase activities in the plasma and pancreas and a better suppressor of increased blood glucose levels.Interestingly, kombucha was noted to induce a marked delay in the absorption of LDL-cholesterol and triglycerides and a significant increase in HDL-cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, Tunisia. aloulou@u.washington.edu

ABSTRACT

Background: Diabetes has become a serious health problem and a major risk factor associated with troublesome health complications, such as metabolism disorders and liver-kidney dysfunctions. The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents. The present study aimed to investigate and compare the hypoglycemic and antilipidemic effects of kombucha and black tea, two natural drinks commonly consumed around the world, in surviving diabetic rats.

Methods: Alloxan diabetic rats were orally supplied with kombucha and black tea at a dose of 5 mL/kg body weight per day for 30 days, fasted overnight, and sacrificed on the 31st day of the experiment. Their bloods were collected and submitted to various biochemical measurements, including blood glucose, cholesterol, triglcerides, urea, creatinine, transaminases, transpeptidase, lipase, and amylase activities. Their pancreases were isolated and processed to measure lipase and α-amylase activities and to perform histological analysis.

Results: The findings revealed that, compared to black tea, kombucha tea was a better inhibitor of α-amylase and lipase activities in the plasma and pancreas and a better suppressor of increased blood glucose levels. Interestingly, kombucha was noted to induce a marked delay in the absorption of LDL-cholesterol and triglycerides and a significant increase in HDL-cholesterol. Histological analyses also showed that it exerted an ameliorative action on the pancreases and efficiently protected the liver-kidney functions of diabetic rats, evidenced by significant decreases in aspartate transaminase, alanine transaminase, and gamma-glytamyl transpeptidase activities in the plasma, as well as in the creatinine and urea contents.

Conclusions: The findings revealed that kombucha tea administration induced attractive curative effects on diabetic rats, particularly in terms of liver-kidney functions. Kombucha tea can, therefore, be considered as a potential strong candidate for future application as a functional supplement for the treatment and prevention of diabetes.

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Effect of KT and BT on α-amylase activity in plasma (A), pancreas (B), and blood glucose levels (C) of surviving diabetic rats. Data represent mean ± S.D (n = 8 for each group). The values are statistically significant and presented as follows: single asterisk (*), p < 0.05 vs. control diabetic rats (Diab); commercial at (@), p < 0.05 vs. respective control rats (Con) [i.e. on the same corresponding tea treatment]; number sign (#), p < 0.05 vs. diabetic rats treated with BT (Diab + BT).
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Figure 2: Effect of KT and BT on α-amylase activity in plasma (A), pancreas (B), and blood glucose levels (C) of surviving diabetic rats. Data represent mean ± S.D (n = 8 for each group). The values are statistically significant and presented as follows: single asterisk (*), p < 0.05 vs. control diabetic rats (Diab); commercial at (@), p < 0.05 vs. respective control rats (Con) [i.e. on the same corresponding tea treatment]; number sign (#), p < 0.05 vs. diabetic rats treated with BT (Diab + BT).

Mentions: Figure 2 shows that, compared to the their counterparts from the normal control group, the rats from the control diabetic rats underwent significant increases in terms of plasma and pancreas α-amylase activities, which reached up to 405 ± 53% (p < 0.05) and 225 ± 52% (p < 0.05), respectively. The black tea supplement administered to diabetic rats was, however, noted to bring about a significant decrease in the plasma and pancreas α-amylase activities, reaching to 52 ± 11% (p < 0.05) and 70 ± 17% (p < 0.05), when compared to untreated diabetic rats. This inhibitory effect of black tea on α-amylase activity was followed by a decrease in the rate of blood glucose that reached up to 65 ± 14% (p < 0.05) (Figure 2C). Considerable α-amylase activity reductions of up to 37 ± 8% (p < 0.05) and 52 ± 7% (p < 0.05) were also observed in the plasma and pancreas of the diabetic rats treated with kombucha, respectively, as compared to those of the untreated diabetic rats. The kombucha supplement was also observed to bring about a significant decrease of 50 ± 11% in terms of blood glucose concentration (p < 0.05) (Figure 2C).


Hypoglycemic and antilipidemic properties of kombucha tea in alloxan-induced diabetic rats.

Aloulou A, Hamden K, Elloumi D, Ali MB, Hargafi K, Jaouadi B, Ayadi F, Elfeki A, Ammar E - BMC Complement Altern Med (2012)

Effect of KT and BT on α-amylase activity in plasma (A), pancreas (B), and blood glucose levels (C) of surviving diabetic rats. Data represent mean ± S.D (n = 8 for each group). The values are statistically significant and presented as follows: single asterisk (*), p < 0.05 vs. control diabetic rats (Diab); commercial at (@), p < 0.05 vs. respective control rats (Con) [i.e. on the same corresponding tea treatment]; number sign (#), p < 0.05 vs. diabetic rats treated with BT (Diab + BT).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3403982&req=5

Figure 2: Effect of KT and BT on α-amylase activity in plasma (A), pancreas (B), and blood glucose levels (C) of surviving diabetic rats. Data represent mean ± S.D (n = 8 for each group). The values are statistically significant and presented as follows: single asterisk (*), p < 0.05 vs. control diabetic rats (Diab); commercial at (@), p < 0.05 vs. respective control rats (Con) [i.e. on the same corresponding tea treatment]; number sign (#), p < 0.05 vs. diabetic rats treated with BT (Diab + BT).
Mentions: Figure 2 shows that, compared to the their counterparts from the normal control group, the rats from the control diabetic rats underwent significant increases in terms of plasma and pancreas α-amylase activities, which reached up to 405 ± 53% (p < 0.05) and 225 ± 52% (p < 0.05), respectively. The black tea supplement administered to diabetic rats was, however, noted to bring about a significant decrease in the plasma and pancreas α-amylase activities, reaching to 52 ± 11% (p < 0.05) and 70 ± 17% (p < 0.05), when compared to untreated diabetic rats. This inhibitory effect of black tea on α-amylase activity was followed by a decrease in the rate of blood glucose that reached up to 65 ± 14% (p < 0.05) (Figure 2C). Considerable α-amylase activity reductions of up to 37 ± 8% (p < 0.05) and 52 ± 7% (p < 0.05) were also observed in the plasma and pancreas of the diabetic rats treated with kombucha, respectively, as compared to those of the untreated diabetic rats. The kombucha supplement was also observed to bring about a significant decrease of 50 ± 11% in terms of blood glucose concentration (p < 0.05) (Figure 2C).

Bottom Line: The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents.The findings revealed that, compared to black tea, kombucha tea was a better inhibitor of α-amylase and lipase activities in the plasma and pancreas and a better suppressor of increased blood glucose levels.Interestingly, kombucha was noted to induce a marked delay in the absorption of LDL-cholesterol and triglycerides and a significant increase in HDL-cholesterol.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, Tunisia. aloulou@u.washington.edu

ABSTRACT

Background: Diabetes has become a serious health problem and a major risk factor associated with troublesome health complications, such as metabolism disorders and liver-kidney dysfunctions. The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents. The present study aimed to investigate and compare the hypoglycemic and antilipidemic effects of kombucha and black tea, two natural drinks commonly consumed around the world, in surviving diabetic rats.

Methods: Alloxan diabetic rats were orally supplied with kombucha and black tea at a dose of 5 mL/kg body weight per day for 30 days, fasted overnight, and sacrificed on the 31st day of the experiment. Their bloods were collected and submitted to various biochemical measurements, including blood glucose, cholesterol, triglcerides, urea, creatinine, transaminases, transpeptidase, lipase, and amylase activities. Their pancreases were isolated and processed to measure lipase and α-amylase activities and to perform histological analysis.

Results: The findings revealed that, compared to black tea, kombucha tea was a better inhibitor of α-amylase and lipase activities in the plasma and pancreas and a better suppressor of increased blood glucose levels. Interestingly, kombucha was noted to induce a marked delay in the absorption of LDL-cholesterol and triglycerides and a significant increase in HDL-cholesterol. Histological analyses also showed that it exerted an ameliorative action on the pancreases and efficiently protected the liver-kidney functions of diabetic rats, evidenced by significant decreases in aspartate transaminase, alanine transaminase, and gamma-glytamyl transpeptidase activities in the plasma, as well as in the creatinine and urea contents.

Conclusions: The findings revealed that kombucha tea administration induced attractive curative effects on diabetic rats, particularly in terms of liver-kidney functions. Kombucha tea can, therefore, be considered as a potential strong candidate for future application as a functional supplement for the treatment and prevention of diabetes.

Show MeSH
Related in: MedlinePlus