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A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth.

Yin XF, Chen J, Mao W, Wang YH, Chen MH - J. Exp. Clin. Cancer Res. (2012)

Bottom Line: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator.Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis.Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on gastric cancer cell growth.

Methods: Gastric cancer cell SGC7901 was treated with DIM at different concentrations (0,10,20,30,40,50 μmol/L) with or without an AhR antagonist, resveratrol. The expression of AhR and Cytochrome P4501A1 (CYP1A1), a classic target gene of AhR pathway, were detected by RT-PCR and Western blot; cell viability was measured by MTT assay, and the changes in cell cycle and apoptosis were analyzed by flow cytometry.

Results: RT-PCR and western-blot showed that with the increase of the concentration of DIM, AhR protein gradually decreased and CYP1A1 expression increased, suggesting that DIM activated the AhR pathway and caused the translocation of AhR from cytoplasm to nucleus. MTT assay indicated that the viability of SGC7901 cells was significantly decreased in a concentration- and time-dependent manner after DIM treatment and this could be partially reversed by resveratrol. Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis.

Conclusion: Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression. AhR may be a potential therapeutic target for gastric cancer treatment.

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The effect of DIM on apoptosis of SGC7901 cells. SGC7901 cells were treated with different concentrations of DIM and subjected to flow cytometric analysis. The results shown are representative of three independent experiments.
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Figure 5: The effect of DIM on apoptosis of SGC7901 cells. SGC7901 cells were treated with different concentrations of DIM and subjected to flow cytometric analysis. The results shown are representative of three independent experiments.

Mentions: 48 h after DIM treatment, the changes of cell apoptosis were observed by flow cytometric analysis. Compared to the control group, cell apoptosis was induced at concentrations of 20 to 50 μmol/L, and the apoptosis rate increased in a dose-dependent manner. These results showed that DIM could induce cell apoptosis in SGC7901 cells (Figure 5 and Table 2).


A selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits gastric cancer cell growth.

Yin XF, Chen J, Mao W, Wang YH, Chen MH - J. Exp. Clin. Cancer Res. (2012)

The effect of DIM on apoptosis of SGC7901 cells. SGC7901 cells were treated with different concentrations of DIM and subjected to flow cytometric analysis. The results shown are representative of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3403951&req=5

Figure 5: The effect of DIM on apoptosis of SGC7901 cells. SGC7901 cells were treated with different concentrations of DIM and subjected to flow cytometric analysis. The results shown are representative of three independent experiments.
Mentions: 48 h after DIM treatment, the changes of cell apoptosis were observed by flow cytometric analysis. Compared to the control group, cell apoptosis was induced at concentrations of 20 to 50 μmol/L, and the apoptosis rate increased in a dose-dependent manner. These results showed that DIM could induce cell apoptosis in SGC7901 cells (Figure 5 and Table 2).

Bottom Line: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator.Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis.Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gastroenterology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with gastric carcinogenesis. 3,3'-Diindolylmethane (DIM) is a relatively non-toxic selective AhR modulator. This study was to detect the effects of DIM on gastric cancer cell growth.

Methods: Gastric cancer cell SGC7901 was treated with DIM at different concentrations (0,10,20,30,40,50 μmol/L) with or without an AhR antagonist, resveratrol. The expression of AhR and Cytochrome P4501A1 (CYP1A1), a classic target gene of AhR pathway, were detected by RT-PCR and Western blot; cell viability was measured by MTT assay, and the changes in cell cycle and apoptosis were analyzed by flow cytometry.

Results: RT-PCR and western-blot showed that with the increase of the concentration of DIM, AhR protein gradually decreased and CYP1A1 expression increased, suggesting that DIM activated the AhR pathway and caused the translocation of AhR from cytoplasm to nucleus. MTT assay indicated that the viability of SGC7901 cells was significantly decreased in a concentration- and time-dependent manner after DIM treatment and this could be partially reversed by resveratrol. Flow cytometry analysis showed that DIM arrested cell cycle in G1 phase and induced cell apoptosis.

Conclusion: Selective aryl hydrocarbon receptor modulator 3,3'-Diindolylmethane inhibits SGC7901 cell proliferation by inducing apoptosis and delaying cell cycle progression. AhR may be a potential therapeutic target for gastric cancer treatment.

Show MeSH
Related in: MedlinePlus