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Loss of vascular early response gene reduces edema formation after experimental stroke.

Liu F, Turtzo LC, Li J, Regard J, Worley P, Zeevi N, McCullough LD - Exp Transl Stroke Med (2012)

Bottom Line: Endothelial cell lines that stably express Verge show enhanced permeability.Increased Verge expression has also been associated with blood brain barrier breakdown.Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, 06030, USA. lmccullough@uchc.edu.

ABSTRACT
Vascular Early Response Gene (Verge) is an immediate early gene (IEG) that is up-regulated in endothelial cells in response to a number of stressors, including ischemic stroke. Endothelial cell lines that stably express Verge show enhanced permeability. Increased Verge expression has also been associated with blood brain barrier breakdown. In this study we investigated the role of Verge in ischemic injury induced by middle cerebral artery occlusion (MCAO) in both Verge knockout (KO) and wild type (WT) mice. Verge KO mice had significantly less cerebral edema formation after MCAO compared to WT mice. However, stroke outcome (infarct size and neurological deficit scores) evaluated at either 24 or 72 hours after stroke showed no differences between the two genotypes. Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.

No MeSH data available.


Related in: MedlinePlus

Edema formation and Stroke Outcomes (A). Absolute water content. (B). Relative water content. *P < 0.05 vs. the ipsilateral hemisphere in WT mice; **P < 0.05 vs. the ipsilateral hemisphere in each group. n = 6/per group. (C&D). Infarct volumes in the cortex, striatum, and whole hemisphere in both Verge KO and WT mice after 24 and 72 hours of MCAO. No significant differences were seen between KO and WT mice. (E). Box-and-whisker plot for NDS at 1.5 and 72 hours of MCAO. NDS was indicated as Median (interquartile range): KO 1.5 h, 3(3–4); WT 1.5 h, 3(3–3.5); KO 72 h, 2(1–2); WT 72 h, 2(1.5–2). *P < 0.05 vs. NDS of 1.5 h in each group. n = 7 ~ 9/per group
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Figure 2: Edema formation and Stroke Outcomes (A). Absolute water content. (B). Relative water content. *P < 0.05 vs. the ipsilateral hemisphere in WT mice; **P < 0.05 vs. the ipsilateral hemisphere in each group. n = 6/per group. (C&D). Infarct volumes in the cortex, striatum, and whole hemisphere in both Verge KO and WT mice after 24 and 72 hours of MCAO. No significant differences were seen between KO and WT mice. (E). Box-and-whisker plot for NDS at 1.5 and 72 hours of MCAO. NDS was indicated as Median (interquartile range): KO 1.5 h, 3(3–4); WT 1.5 h, 3(3–3.5); KO 72 h, 2(1–2); WT 72 h, 2(1.5–2). *P < 0.05 vs. NDS of 1.5 h in each group. n = 7 ~ 9/per group

Mentions: Absolute water content in the ipsilateral (stroke) hemisphere of both KO and WT mice was significantly increased compared with that of the contralateral hemisphere after stroke (Figure 2A). Verge KO mice had significantly less water content in the ipsilateral hemisphere compared to WT mice. The absolute water content in the contralateral hemisphere of KO mice was also less than that in WT mice although this difference was not significant. We also calculated the relative water content, comparing each genotype to its contralateral (non-stroke) hemisphere, which showed no difference between KO and WT mice (Figure 2B).


Loss of vascular early response gene reduces edema formation after experimental stroke.

Liu F, Turtzo LC, Li J, Regard J, Worley P, Zeevi N, McCullough LD - Exp Transl Stroke Med (2012)

Edema formation and Stroke Outcomes (A). Absolute water content. (B). Relative water content. *P < 0.05 vs. the ipsilateral hemisphere in WT mice; **P < 0.05 vs. the ipsilateral hemisphere in each group. n = 6/per group. (C&D). Infarct volumes in the cortex, striatum, and whole hemisphere in both Verge KO and WT mice after 24 and 72 hours of MCAO. No significant differences were seen between KO and WT mice. (E). Box-and-whisker plot for NDS at 1.5 and 72 hours of MCAO. NDS was indicated as Median (interquartile range): KO 1.5 h, 3(3–4); WT 1.5 h, 3(3–3.5); KO 72 h, 2(1–2); WT 72 h, 2(1.5–2). *P < 0.05 vs. NDS of 1.5 h in each group. n = 7 ~ 9/per group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3403842&req=5

Figure 2: Edema formation and Stroke Outcomes (A). Absolute water content. (B). Relative water content. *P < 0.05 vs. the ipsilateral hemisphere in WT mice; **P < 0.05 vs. the ipsilateral hemisphere in each group. n = 6/per group. (C&D). Infarct volumes in the cortex, striatum, and whole hemisphere in both Verge KO and WT mice after 24 and 72 hours of MCAO. No significant differences were seen between KO and WT mice. (E). Box-and-whisker plot for NDS at 1.5 and 72 hours of MCAO. NDS was indicated as Median (interquartile range): KO 1.5 h, 3(3–4); WT 1.5 h, 3(3–3.5); KO 72 h, 2(1–2); WT 72 h, 2(1.5–2). *P < 0.05 vs. NDS of 1.5 h in each group. n = 7 ~ 9/per group
Mentions: Absolute water content in the ipsilateral (stroke) hemisphere of both KO and WT mice was significantly increased compared with that of the contralateral hemisphere after stroke (Figure 2A). Verge KO mice had significantly less water content in the ipsilateral hemisphere compared to WT mice. The absolute water content in the contralateral hemisphere of KO mice was also less than that in WT mice although this difference was not significant. We also calculated the relative water content, comparing each genotype to its contralateral (non-stroke) hemisphere, which showed no difference between KO and WT mice (Figure 2B).

Bottom Line: Endothelial cell lines that stably express Verge show enhanced permeability.Increased Verge expression has also been associated with blood brain barrier breakdown.Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, 06030, USA. lmccullough@uchc.edu.

ABSTRACT
Vascular Early Response Gene (Verge) is an immediate early gene (IEG) that is up-regulated in endothelial cells in response to a number of stressors, including ischemic stroke. Endothelial cell lines that stably express Verge show enhanced permeability. Increased Verge expression has also been associated with blood brain barrier breakdown. In this study we investigated the role of Verge in ischemic injury induced by middle cerebral artery occlusion (MCAO) in both Verge knockout (KO) and wild type (WT) mice. Verge KO mice had significantly less cerebral edema formation after MCAO compared to WT mice. However, stroke outcome (infarct size and neurological deficit scores) evaluated at either 24 or 72 hours after stroke showed no differences between the two genotypes. Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.

No MeSH data available.


Related in: MedlinePlus