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Expression of MUC5AC and Trefoil Peptide 1 (TFF1) in the Subtypes of Intestinal Metaplasia.

Song JY, Kim BW, Lee AW, Lee KY, Chung IS, Lee BI, Choi H, Ji JS, Chae HS, Choi KY - Clin Endosc (2012)

Bottom Line: Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM.Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM.IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Alterations of the expression pattern of mucins and trefoil peptides have been described in gastric adenocarcinomas and in their precursor lesions. The aim of this study was to determine the progression patterns of intestinal metaplasia (IM) subtypes by analyzing the expression patterns of TFF1 and MUC5AC in different subtypes of IM of the stomach.

Methods: Endoscopic gastric biopsies of the antrum and body were obtained from patients with dyspepsia and endoscopic IM. Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM. Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM.

Results: IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples. There was a gradual decrease in MUC5AC and TFF1 expression during the progression of IM from type I to type III via the type II intermediate.

Conclusions: This downregulation of MUC5AC and TFF1 expression may challenge the sequential progression of IM from type I to type III via the type II intermediate, and it might be associated with gastric carcinogenesis.

No MeSH data available.


Related in: MedlinePlus

Examples of MUC5AC expression as determined by immunohistochemistry (×200).
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Figure 2: Examples of MUC5AC expression as determined by immunohistochemistry (×200).

Mentions: Immunohistochemical reactivity to MUC5AC decreased during the progression from type I to type III both in the corpus and in the antrum (Fig. 1). MUC5AC was mainly expressed in columnar epithelial cells. Examples of immunohistochemical reactivity to MUC5AC are presented in Fig. 2. Immunohistochemical reactivity to TFF1 also decreased during the progression from type I to type III both in the corpus and in the antrum (Fig. 3). TFF1 was mainly expressed in foveolar cells. Examples of immunohistochemical reactivity to TFF1 are presented in Fig. 4.


Expression of MUC5AC and Trefoil Peptide 1 (TFF1) in the Subtypes of Intestinal Metaplasia.

Song JY, Kim BW, Lee AW, Lee KY, Chung IS, Lee BI, Choi H, Ji JS, Chae HS, Choi KY - Clin Endosc (2012)

Examples of MUC5AC expression as determined by immunohistochemistry (×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3401619&req=5

Figure 2: Examples of MUC5AC expression as determined by immunohistochemistry (×200).
Mentions: Immunohistochemical reactivity to MUC5AC decreased during the progression from type I to type III both in the corpus and in the antrum (Fig. 1). MUC5AC was mainly expressed in columnar epithelial cells. Examples of immunohistochemical reactivity to MUC5AC are presented in Fig. 2. Immunohistochemical reactivity to TFF1 also decreased during the progression from type I to type III both in the corpus and in the antrum (Fig. 3). TFF1 was mainly expressed in foveolar cells. Examples of immunohistochemical reactivity to TFF1 are presented in Fig. 4.

Bottom Line: Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM.Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM.IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT

Background/aims: Alterations of the expression pattern of mucins and trefoil peptides have been described in gastric adenocarcinomas and in their precursor lesions. The aim of this study was to determine the progression patterns of intestinal metaplasia (IM) subtypes by analyzing the expression patterns of TFF1 and MUC5AC in different subtypes of IM of the stomach.

Methods: Endoscopic gastric biopsies of the antrum and body were obtained from patients with dyspepsia and endoscopic IM. Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM. Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM.

Results: IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples. There was a gradual decrease in MUC5AC and TFF1 expression during the progression of IM from type I to type III via the type II intermediate.

Conclusions: This downregulation of MUC5AC and TFF1 expression may challenge the sequential progression of IM from type I to type III via the type II intermediate, and it might be associated with gastric carcinogenesis.

No MeSH data available.


Related in: MedlinePlus