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Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection.

Baton LA, Ranford-Cartwright LC - Int. J. Parasitol. (2012)

Bottom Line: Similar densities of macrogametes/zygotes, and immature retort-form and mature ookinetes were found within the bloodmeals of both mosquito species.However, in A. albimanus, ookinetes were seldom associated with the peritrophic matrix, and were neither observed in the ectoperitrophic space nor the midgut epithelium.Vital staining of the immature retort-form and mature ookinetes found within the luminal bloodmeal, demonstrated that a significantly greater proportion of these malaria parasite stages were non-viable in A. albimanus compared with A. stephensi.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Sir Graeme Davies Building, University of Glasgow, Glasgow, UK. lukebaton@mailcity.com

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Comparison of the viability of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means of the proportions of propidium iodide (PI)-negative (viable) malaria parasites and their associated 95% confidence intervals. For each mosquito species, midguts from 12 individuals were assayed for each time point (h post-bloodfeeding). For each developmental stage, 50–100 parasites were counted per midgut or, for later time points when parasite numbers declined, as many parasites as were present. The data are pooled from three independent experimental feeds. The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05).
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f0030: Comparison of the viability of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means of the proportions of propidium iodide (PI)-negative (viable) malaria parasites and their associated 95% confidence intervals. For each mosquito species, midguts from 12 individuals were assayed for each time point (h post-bloodfeeding). For each developmental stage, 50–100 parasites were counted per midgut or, for later time points when parasite numbers declined, as many parasites as were present. The data are pooled from three independent experimental feeds. The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05).

Mentions: In order to further characterise the causes of P. falciparum clone 3D7A loss within A. albimanus and A. stephensi, the viability of the three different malaria parasite developmental stages present within the midgut lumen were compared using vital staining in three independent experimental feeds (Fig. 6). Within approximately the first 24 h pbf, the majority of round forms, and immature retort-form and mature ookinetes were negative for PI in both mosquito species, implying that most of these malaria parasite stages were viable during this period. Furthermore, the proportion of PI-positive parasites did not differ significantly between the two mosquito species during this time (Fig. 6). However from approximately 24 h pbf onwards, the proportion of malaria parasites positive for PI was significantly higher in A. albimanus than A. stephensi for each of the three different developmental stages (Fig. 6), indicating reduced viability of these malaria parasite stages in the midgut lumen of A. albimanus during this period.


Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection.

Baton LA, Ranford-Cartwright LC - Int. J. Parasitol. (2012)

Comparison of the viability of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means of the proportions of propidium iodide (PI)-negative (viable) malaria parasites and their associated 95% confidence intervals. For each mosquito species, midguts from 12 individuals were assayed for each time point (h post-bloodfeeding). For each developmental stage, 50–100 parasites were counted per midgut or, for later time points when parasite numbers declined, as many parasites as were present. The data are pooled from three independent experimental feeds. The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3401372&req=5

f0030: Comparison of the viability of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means of the proportions of propidium iodide (PI)-negative (viable) malaria parasites and their associated 95% confidence intervals. For each mosquito species, midguts from 12 individuals were assayed for each time point (h post-bloodfeeding). For each developmental stage, 50–100 parasites were counted per midgut or, for later time points when parasite numbers declined, as many parasites as were present. The data are pooled from three independent experimental feeds. The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05).
Mentions: In order to further characterise the causes of P. falciparum clone 3D7A loss within A. albimanus and A. stephensi, the viability of the three different malaria parasite developmental stages present within the midgut lumen were compared using vital staining in three independent experimental feeds (Fig. 6). Within approximately the first 24 h pbf, the majority of round forms, and immature retort-form and mature ookinetes were negative for PI in both mosquito species, implying that most of these malaria parasite stages were viable during this period. Furthermore, the proportion of PI-positive parasites did not differ significantly between the two mosquito species during this time (Fig. 6). However from approximately 24 h pbf onwards, the proportion of malaria parasites positive for PI was significantly higher in A. albimanus than A. stephensi for each of the three different developmental stages (Fig. 6), indicating reduced viability of these malaria parasite stages in the midgut lumen of A. albimanus during this period.

Bottom Line: Similar densities of macrogametes/zygotes, and immature retort-form and mature ookinetes were found within the bloodmeals of both mosquito species.However, in A. albimanus, ookinetes were seldom associated with the peritrophic matrix, and were neither observed in the ectoperitrophic space nor the midgut epithelium.Vital staining of the immature retort-form and mature ookinetes found within the luminal bloodmeal, demonstrated that a significantly greater proportion of these malaria parasite stages were non-viable in A. albimanus compared with A. stephensi.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Sir Graeme Davies Building, University of Glasgow, Glasgow, UK. lukebaton@mailcity.com

Show MeSH
Related in: MedlinePlus