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Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection.

Baton LA, Ranford-Cartwright LC - Int. J. Parasitol. (2012)

Bottom Line: Similar densities of macrogametes/zygotes, and immature retort-form and mature ookinetes were found within the bloodmeals of both mosquito species.However, in A. albimanus, ookinetes were seldom associated with the peritrophic matrix, and were neither observed in the ectoperitrophic space nor the midgut epithelium.Vital staining of the immature retort-form and mature ookinetes found within the luminal bloodmeal, demonstrated that a significantly greater proportion of these malaria parasite stages were non-viable in A. albimanus compared with A. stephensi.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Sir Graeme Davies Building, University of Glasgow, Glasgow, UK. lukebaton@mailcity.com

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Comparison of the densities and kinetics of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means and their associated standard errors. For each mosquito species, midguts from six individuals were assayed for each time point (h post-bloodfeeding (pbf)). The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05). The data shown are for one experiment. A second experiment gave similar results, showing the same general pattern of change over time in malaria parasite densities, although the absolute numbers of malaria parasites were lower (data not shown).
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f0005: Comparison of the densities and kinetics of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means and their associated standard errors. For each mosquito species, midguts from six individuals were assayed for each time point (h post-bloodfeeding (pbf)). The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05). The data shown are for one experiment. A second experiment gave similar results, showing the same general pattern of change over time in malaria parasite densities, although the absolute numbers of malaria parasites were lower (data not shown).

Mentions: Development of P. falciparum clone 3D7A within the bloodmeals of A. albimanus and A. stephensi was compared in two independent experimental feeds (Fig. 1). The formation of round forms, immature retort-form ookinetes and mature ookinetes within the bloodmeal was similar in both mosquito species, with regard to both the timing and the number of malaria parasite stages formed (Fig. 1). The density of round forms was highest immediately after bloodfeeding and declined consistently thereafter with increasing time pbf (Fig. 1A), paralleling the decline in the mean total protein content per midgut (Fig. 2). The density of retort-form ookinetes was initially low, rose to a peak at 12 h pbf and then declined (Fig. 1B), while mature ookinetes were first observed at 18 h pbf, with densities peaking at 24 h pbf before declining thereafter (Fig. 1C). The peak densities of round forms (6 h pbf), retort-form ookinetes (12 h pbf) and mature ookinetes (24 h pbf) did not differ significantly between A. albimanus and A. stephensi (t test, P > 0.05 in all instances). However, with increasing time pbf, the density of the three distinct developmental stages of the malaria parasite differed significantly between the two mosquito species, being markedly lower in A. albimanus compared with A. stephensi. In particular, the densities of retort-form and mature ookinetes were both significantly lower within the bloodmeals of A. albimanus from, respectively, 18 and 30 h pbf onwards (t test, P < 0.05 in all instances) (Fig. 1B and C). Comparison of the mean total protein content per midgut with increasing time pbf indicated that bloodmeal digestion occurred more rapidly and was completed sooner in A. albimanus than A. stephensi (Fig. 2).


Ookinete destruction within the mosquito midgut lumen explains Anopheles albimanus refractoriness to Plasmodium falciparum (3D7A) oocyst infection.

Baton LA, Ranford-Cartwright LC - Int. J. Parasitol. (2012)

Comparison of the densities and kinetics of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means and their associated standard errors. For each mosquito species, midguts from six individuals were assayed for each time point (h post-bloodfeeding (pbf)). The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05). The data shown are for one experiment. A second experiment gave similar results, showing the same general pattern of change over time in malaria parasite densities, although the absolute numbers of malaria parasites were lower (data not shown).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3401372&req=5

f0005: Comparison of the densities and kinetics of the three developmental stages (A–C) of Plasmodium falciparum clone 3D7A occurring within the midgut lumens of Anopheles albimanus and Anopheles stephensi. Values shown are arithmetic means and their associated standard errors. For each mosquito species, midguts from six individuals were assayed for each time point (h post-bloodfeeding (pbf)). The shaded area indicates time points that differed significantly between the two mosquito species (t test, P < 0.05). The data shown are for one experiment. A second experiment gave similar results, showing the same general pattern of change over time in malaria parasite densities, although the absolute numbers of malaria parasites were lower (data not shown).
Mentions: Development of P. falciparum clone 3D7A within the bloodmeals of A. albimanus and A. stephensi was compared in two independent experimental feeds (Fig. 1). The formation of round forms, immature retort-form ookinetes and mature ookinetes within the bloodmeal was similar in both mosquito species, with regard to both the timing and the number of malaria parasite stages formed (Fig. 1). The density of round forms was highest immediately after bloodfeeding and declined consistently thereafter with increasing time pbf (Fig. 1A), paralleling the decline in the mean total protein content per midgut (Fig. 2). The density of retort-form ookinetes was initially low, rose to a peak at 12 h pbf and then declined (Fig. 1B), while mature ookinetes were first observed at 18 h pbf, with densities peaking at 24 h pbf before declining thereafter (Fig. 1C). The peak densities of round forms (6 h pbf), retort-form ookinetes (12 h pbf) and mature ookinetes (24 h pbf) did not differ significantly between A. albimanus and A. stephensi (t test, P > 0.05 in all instances). However, with increasing time pbf, the density of the three distinct developmental stages of the malaria parasite differed significantly between the two mosquito species, being markedly lower in A. albimanus compared with A. stephensi. In particular, the densities of retort-form and mature ookinetes were both significantly lower within the bloodmeals of A. albimanus from, respectively, 18 and 30 h pbf onwards (t test, P < 0.05 in all instances) (Fig. 1B and C). Comparison of the mean total protein content per midgut with increasing time pbf indicated that bloodmeal digestion occurred more rapidly and was completed sooner in A. albimanus than A. stephensi (Fig. 2).

Bottom Line: Similar densities of macrogametes/zygotes, and immature retort-form and mature ookinetes were found within the bloodmeals of both mosquito species.However, in A. albimanus, ookinetes were seldom associated with the peritrophic matrix, and were neither observed in the ectoperitrophic space nor the midgut epithelium.Vital staining of the immature retort-form and mature ookinetes found within the luminal bloodmeal, demonstrated that a significantly greater proportion of these malaria parasite stages were non-viable in A. albimanus compared with A. stephensi.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Sir Graeme Davies Building, University of Glasgow, Glasgow, UK. lukebaton@mailcity.com

Show MeSH
Related in: MedlinePlus