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Moth sex pheromone receptors and deceitful parapheromones.

Xu P, Garczynski SF, Atungulu E, Syed Z, Choo YM, Vidal DM, Zitelli CH, Leal WS - PLoS ONE (2012)

Bottom Line: AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol.Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone.A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

View Article: PubMed Central - PubMed

Affiliation: Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America.

ABSTRACT
The insect's olfactory system is so selective that male moths, for example, can discriminate female-produced sex pheromones from compounds with minimal structural modifications. Yet, there is an exception for this "lock-and-key" tight selectivity. Formate analogs can be used as replacement for less chemically stable, long-chain aldehyde pheromones, because male moths respond physiologically and behaviorally to these parapheromones. However, it remained hitherto unknown how formate analogs interact with aldehyde-sensitive odorant receptors (ORs). Neuronal responses to semiochemicals were investigated with single sensillum recordings. Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. Quality, relative quantity, and purity of samples were evaluated by gas chromatography and gas chromatography-mass spectrometry. We identified olfactory receptor neurons (ORNs) housed in trichoid sensilla on the antennae of male navel orangeworm that responded equally to the main constituent of the sex pheromone, (11Z,13Z)-hexadecadienal (Z11Z13-16Ald), and its formate analog, (9Z,11Z)-tetradecen-1-yl formate (Z9Z11-14OFor). We cloned an odorant receptor co-receptor (Orco) and aldehyde-sensitive ORs from the navel orangeworm, one of which (AtraOR1) was expressed specifically in male antennae. AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol. Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone. A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

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Dose-dependent current responses obtained with AtraOR1•AtraOrco-expressing oocytes.Note the curve generated by challenging the oocytes with the formate analog is shifted at least one order of magnitude.
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pone-0041653-g005: Dose-dependent current responses obtained with AtraOR1•AtraOrco-expressing oocytes.Note the curve generated by challenging the oocytes with the formate analog is shifted at least one order of magnitude.

Mentions: Surprisingly, AtraOR1•AtraOrco-expressing oocytes responded more strongly to the synthetic formate analog, Z9Z11-14OFor, than to the natural sex pheromone component Z11Z13-16Ald (Figure 5). The formate analog showed not only a lower threshold, but the dose-dependence curve for the parapheromone was shifted by at least one order of magnitude, with EC50 of 499 nM and 90 nM for aldehyde and formate, respectively.


Moth sex pheromone receptors and deceitful parapheromones.

Xu P, Garczynski SF, Atungulu E, Syed Z, Choo YM, Vidal DM, Zitelli CH, Leal WS - PLoS ONE (2012)

Dose-dependent current responses obtained with AtraOR1•AtraOrco-expressing oocytes.Note the curve generated by challenging the oocytes with the formate analog is shifted at least one order of magnitude.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3401280&req=5

pone-0041653-g005: Dose-dependent current responses obtained with AtraOR1•AtraOrco-expressing oocytes.Note the curve generated by challenging the oocytes with the formate analog is shifted at least one order of magnitude.
Mentions: Surprisingly, AtraOR1•AtraOrco-expressing oocytes responded more strongly to the synthetic formate analog, Z9Z11-14OFor, than to the natural sex pheromone component Z11Z13-16Ald (Figure 5). The formate analog showed not only a lower threshold, but the dose-dependence curve for the parapheromone was shifted by at least one order of magnitude, with EC50 of 499 nM and 90 nM for aldehyde and formate, respectively.

Bottom Line: AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol.Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone.A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

View Article: PubMed Central - PubMed

Affiliation: Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America.

ABSTRACT
The insect's olfactory system is so selective that male moths, for example, can discriminate female-produced sex pheromones from compounds with minimal structural modifications. Yet, there is an exception for this "lock-and-key" tight selectivity. Formate analogs can be used as replacement for less chemically stable, long-chain aldehyde pheromones, because male moths respond physiologically and behaviorally to these parapheromones. However, it remained hitherto unknown how formate analogs interact with aldehyde-sensitive odorant receptors (ORs). Neuronal responses to semiochemicals were investigated with single sensillum recordings. Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. Quality, relative quantity, and purity of samples were evaluated by gas chromatography and gas chromatography-mass spectrometry. We identified olfactory receptor neurons (ORNs) housed in trichoid sensilla on the antennae of male navel orangeworm that responded equally to the main constituent of the sex pheromone, (11Z,13Z)-hexadecadienal (Z11Z13-16Ald), and its formate analog, (9Z,11Z)-tetradecen-1-yl formate (Z9Z11-14OFor). We cloned an odorant receptor co-receptor (Orco) and aldehyde-sensitive ORs from the navel orangeworm, one of which (AtraOR1) was expressed specifically in male antennae. AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol. Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone. A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

Show MeSH