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Moth sex pheromone receptors and deceitful parapheromones.

Xu P, Garczynski SF, Atungulu E, Syed Z, Choo YM, Vidal DM, Zitelli CH, Leal WS - PLoS ONE (2012)

Bottom Line: AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol.Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone.A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

View Article: PubMed Central - PubMed

Affiliation: Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America.

ABSTRACT
The insect's olfactory system is so selective that male moths, for example, can discriminate female-produced sex pheromones from compounds with minimal structural modifications. Yet, there is an exception for this "lock-and-key" tight selectivity. Formate analogs can be used as replacement for less chemically stable, long-chain aldehyde pheromones, because male moths respond physiologically and behaviorally to these parapheromones. However, it remained hitherto unknown how formate analogs interact with aldehyde-sensitive odorant receptors (ORs). Neuronal responses to semiochemicals were investigated with single sensillum recordings. Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. Quality, relative quantity, and purity of samples were evaluated by gas chromatography and gas chromatography-mass spectrometry. We identified olfactory receptor neurons (ORNs) housed in trichoid sensilla on the antennae of male navel orangeworm that responded equally to the main constituent of the sex pheromone, (11Z,13Z)-hexadecadienal (Z11Z13-16Ald), and its formate analog, (9Z,11Z)-tetradecen-1-yl formate (Z9Z11-14OFor). We cloned an odorant receptor co-receptor (Orco) and aldehyde-sensitive ORs from the navel orangeworm, one of which (AtraOR1) was expressed specifically in male antennae. AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol. Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone. A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

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Responses of the peripheral olfactory system of the navel orangeworm to the major constituent of the sex pheromone and its formate analog.(A) Extracellularly recorded single unit responses from ORNs housed in a trichoid sensillum on the antennae, and (B) dose-dependent relationships (n = 5; error bar in all figures represent SEM).
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pone-0041653-g001: Responses of the peripheral olfactory system of the navel orangeworm to the major constituent of the sex pheromone and its formate analog.(A) Extracellularly recorded single unit responses from ORNs housed in a trichoid sensillum on the antennae, and (B) dose-dependent relationships (n = 5; error bar in all figures represent SEM).

Mentions: The neurons sensitive to Z11Z13-16Ald were also activated by the formate analog, Z9Z11-14For, with these compounds generating indistinguishable dose-dependent curves (Figure 1). Although not surprising in view of previous observations with other species [11], [12], it is interesting that the olfactory system of male moths is so selective towards pheromones [5] yet it is “deceived” by a pheromone analog. These findings prompted us to investigate whether this “relaxed selectivity” was entirely manifested at the level of an odorant receptor (OR) sensitive to the main constituent of the sex pheromone.


Moth sex pheromone receptors and deceitful parapheromones.

Xu P, Garczynski SF, Atungulu E, Syed Z, Choo YM, Vidal DM, Zitelli CH, Leal WS - PLoS ONE (2012)

Responses of the peripheral olfactory system of the navel orangeworm to the major constituent of the sex pheromone and its formate analog.(A) Extracellularly recorded single unit responses from ORNs housed in a trichoid sensillum on the antennae, and (B) dose-dependent relationships (n = 5; error bar in all figures represent SEM).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3401280&req=5

pone-0041653-g001: Responses of the peripheral olfactory system of the navel orangeworm to the major constituent of the sex pheromone and its formate analog.(A) Extracellularly recorded single unit responses from ORNs housed in a trichoid sensillum on the antennae, and (B) dose-dependent relationships (n = 5; error bar in all figures represent SEM).
Mentions: The neurons sensitive to Z11Z13-16Ald were also activated by the formate analog, Z9Z11-14For, with these compounds generating indistinguishable dose-dependent curves (Figure 1). Although not surprising in view of previous observations with other species [11], [12], it is interesting that the olfactory system of male moths is so selective towards pheromones [5] yet it is “deceived” by a pheromone analog. These findings prompted us to investigate whether this “relaxed selectivity” was entirely manifested at the level of an odorant receptor (OR) sensitive to the main constituent of the sex pheromone.

Bottom Line: AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol.Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone.A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

View Article: PubMed Central - PubMed

Affiliation: Honorary Maeda-Duffey Laboratory, University of California Davis, Davis, California, United States of America.

ABSTRACT
The insect's olfactory system is so selective that male moths, for example, can discriminate female-produced sex pheromones from compounds with minimal structural modifications. Yet, there is an exception for this "lock-and-key" tight selectivity. Formate analogs can be used as replacement for less chemically stable, long-chain aldehyde pheromones, because male moths respond physiologically and behaviorally to these parapheromones. However, it remained hitherto unknown how formate analogs interact with aldehyde-sensitive odorant receptors (ORs). Neuronal responses to semiochemicals were investigated with single sensillum recordings. Odorant receptors (ORs) were cloned using degenerate primers, and tested with the Xenopus oocyte expression system. Quality, relative quantity, and purity of samples were evaluated by gas chromatography and gas chromatography-mass spectrometry. We identified olfactory receptor neurons (ORNs) housed in trichoid sensilla on the antennae of male navel orangeworm that responded equally to the main constituent of the sex pheromone, (11Z,13Z)-hexadecadienal (Z11Z13-16Ald), and its formate analog, (9Z,11Z)-tetradecen-1-yl formate (Z9Z11-14OFor). We cloned an odorant receptor co-receptor (Orco) and aldehyde-sensitive ORs from the navel orangeworm, one of which (AtraOR1) was expressed specifically in male antennae. AtraOR1•AtraOrco-expressing oocytes responded mainly to Z11Z13-16Ald, with moderate sensitivity to another component of the sex pheromone, (11Z,13Z)-hexadecadien-1-ol. Surprisingly, this receptor was more sensitive to the related formate than to the natural sex pheromone. A pheromone receptor from Heliothis virescens, HR13 ( = HvirOR13) showed a similar profile, with stronger responses elicited by a formate analog than to the natural sex pheromone, (11Z)-hexadecenal thus suggesting this might be a common feature of moth pheromone receptors.

Show MeSH