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The sensitivity and specificity of hyperglycosylated hCG (hhCG) levels to reliably diagnose clinical IVF pregnancies at 6 days following embryo transfer.

Strom CM, Bonilla-Guererro R, Zhang K, Doody KJ, Tourgeman D, Alvero R, Cedars MI, Crossley B, Pandian R, Sharma R, Neidich J, Salazar D - J. Assist. Reprod. Genet. (2012)

Bottom Line: For blastocyst transfers, a single serum or urine hhCG measurement identified pregnancies (both biochemical and clinical) at 6 days post ET with 100% sensitivity and specificity.There were 6 biochemical pregnancies, all following blastocyst transfers.All of these pregnancies were identified by lower values.

View Article: PubMed Central - PubMed

Affiliation: Quest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675-2042, USA. charles.m.strom@questdiagnostics.com

ABSTRACT

Objective: To determine the sensitivity and specificity of hyperglycosylated hCG (hhCG) measurements for the diagnosis of clinical pregnancies in the IVF setting and how soon post embryo transfer (ET) a pregnancy can be detected using an ultrasensitive (hhCG) assay. To determine if a single, early hhCG measurement can discriminate between biochemical and clinical pregnancies.

Design: A 4 center prospective blinded clinical trial was performed with patients undergoing IVF-ET. Patients had blood drawn and submitted for hhCG analysis on the day of ET and at days 4, 6, 8, and 12 thereafter. First morning urines were collected and submitted for hhCG analysis on days 0, 4, 6, 8, 10 and 12.

Setting: Fertility Centers

Outcome measures: Clinical pregnancies were defined as an ultrasound study demonstrating a gestational sac and/or heart beat at appropriate gestational ages.

Results: Fifty-six of 58 enrolled patients completed the study. There were 25 clinical and 6 biochemical pregnancies. For blastocyst transfers, a single serum or urine hhCG measurement identified pregnancies (both biochemical and clinical) at 6 days post ET with 100% sensitivity and specificity. There were 6 biochemical pregnancies, all following blastocyst transfers. All of these pregnancies were identified by lower values.

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Related in: MedlinePlus

Kinetics of urine hhCG levels following embryo transfer. a Blastocyst transfer. Triangles = non-pregnant. Diamonds = Biochemical pregnancies. Circles = Clinical pregnancies. b Cleavage stage Embryo Transfer. Triangles: Non-pregnant. Circles: Clinical pregnancies
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Fig2: Kinetics of urine hhCG levels following embryo transfer. a Blastocyst transfer. Triangles = non-pregnant. Diamonds = Biochemical pregnancies. Circles = Clinical pregnancies. b Cleavage stage Embryo Transfer. Triangles: Non-pregnant. Circles: Clinical pregnancies

Mentions: FigureĀ 2a and b demonstrate the kinetics of urine hhCG values during the post ET period. Although the curves show similar trends, the difference in urine hhCG values between clincal and biochemical pregnancies is not nearly as pronounced as for serum and urine hhCG values cannot discriminate between them.Fig. 2


The sensitivity and specificity of hyperglycosylated hCG (hhCG) levels to reliably diagnose clinical IVF pregnancies at 6 days following embryo transfer.

Strom CM, Bonilla-Guererro R, Zhang K, Doody KJ, Tourgeman D, Alvero R, Cedars MI, Crossley B, Pandian R, Sharma R, Neidich J, Salazar D - J. Assist. Reprod. Genet. (2012)

Kinetics of urine hhCG levels following embryo transfer. a Blastocyst transfer. Triangles = non-pregnant. Diamonds = Biochemical pregnancies. Circles = Clinical pregnancies. b Cleavage stage Embryo Transfer. Triangles: Non-pregnant. Circles: Clinical pregnancies
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3401259&req=5

Fig2: Kinetics of urine hhCG levels following embryo transfer. a Blastocyst transfer. Triangles = non-pregnant. Diamonds = Biochemical pregnancies. Circles = Clinical pregnancies. b Cleavage stage Embryo Transfer. Triangles: Non-pregnant. Circles: Clinical pregnancies
Mentions: FigureĀ 2a and b demonstrate the kinetics of urine hhCG values during the post ET period. Although the curves show similar trends, the difference in urine hhCG values between clincal and biochemical pregnancies is not nearly as pronounced as for serum and urine hhCG values cannot discriminate between them.Fig. 2

Bottom Line: For blastocyst transfers, a single serum or urine hhCG measurement identified pregnancies (both biochemical and clinical) at 6 days post ET with 100% sensitivity and specificity.There were 6 biochemical pregnancies, all following blastocyst transfers.All of these pregnancies were identified by lower values.

View Article: PubMed Central - PubMed

Affiliation: Quest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675-2042, USA. charles.m.strom@questdiagnostics.com

ABSTRACT

Objective: To determine the sensitivity and specificity of hyperglycosylated hCG (hhCG) measurements for the diagnosis of clinical pregnancies in the IVF setting and how soon post embryo transfer (ET) a pregnancy can be detected using an ultrasensitive (hhCG) assay. To determine if a single, early hhCG measurement can discriminate between biochemical and clinical pregnancies.

Design: A 4 center prospective blinded clinical trial was performed with patients undergoing IVF-ET. Patients had blood drawn and submitted for hhCG analysis on the day of ET and at days 4, 6, 8, and 12 thereafter. First morning urines were collected and submitted for hhCG analysis on days 0, 4, 6, 8, 10 and 12.

Setting: Fertility Centers

Outcome measures: Clinical pregnancies were defined as an ultrasound study demonstrating a gestational sac and/or heart beat at appropriate gestational ages.

Results: Fifty-six of 58 enrolled patients completed the study. There were 25 clinical and 6 biochemical pregnancies. For blastocyst transfers, a single serum or urine hhCG measurement identified pregnancies (both biochemical and clinical) at 6 days post ET with 100% sensitivity and specificity. There were 6 biochemical pregnancies, all following blastocyst transfers. All of these pregnancies were identified by lower values.

Show MeSH
Related in: MedlinePlus