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Effect of magnetic stimulation in spinal cord on limb angiogenesis and implication: a pilot study.

Lee D, Beom J, Oh BM, Seo KS - Ann Rehabil Med (2012)

Bottom Line: Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026).Expression of eNOS in left plus right quadriceps markedly increased in group 3 and 4 compared with group 1 and 2 (p=0.007).Repetitive magnetic stimulation of the spinal cord may exert an angiogenic effect closely linked to lymphangiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul 110-744, Korea.

ABSTRACT

Objective: To investigate the effect of repetitive magnetic stimulation (rMS) of the spinal cord on limb angiogenesis in healthy rats and explore its implication for the treatment of lymphedema.

Method: Twelve adult male Sprague-Dawley rats were divided into four groups as follows: sham rMS followed by tissue harvest 5 minutes later (group 1, n=2), 1 Hz rMS and tissue harvest 5 minutes later (group 2, n=3), 20 Hz rMS and tissue harvest 5 minutes later (group 3, n=3), 20 Hz rMS and tissue harvest 30 minutes later (group 4, n=4). Animals were treated with 20-minute rMS with 120% of the motor threshold on their left side of upper lumbar spinal cord. Expression of angiogenic factors, that is, Akt, phospho-Akt (pAkt), endothelial nitric oxide synthase (eNOS), phospho-eNOS (p-eNOS) were measured by western blot. Bilateral hindlimb muscles (quadriceps and gastrocnemius) were harvested.

Results: Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left plus right quadriceps markedly increased in group 3 and 4 compared with group 1 and 2 (p=0.007). Expressions of eNOS, Akt and p-eNOS, pAkt in gastrocnemius were not comparable between four groups (p>0.05).

Conclusion: Repetitive magnetic stimulation of the spinal cord may exert an angiogenic effect closely linked to lymphangiogenesis. It has clinical implication for the possible therapy of lymphedema caused by breast, cervical or endometrial cancer operation. Future studies with the specific lymphatic endothelial cell markers are required to confirm the effect of rMS on lymphangiogenesis.

No MeSH data available.


Related in: MedlinePlus

eNOS and Akt expressions in quadriceps muscle. Western blots of left (A) and right (B) quadriceps, eNOS (C) and Akt (D) expressions in left quadriceps, eNOS (E) and Akt (F) expressions in left+right quadriceps. Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left+right quadriceps markedly increased in group 3 and 4 (6.4 and 7.7-fold each compared with group 1, 3.5 and 4.2-fold each compared with group 2, p=0.007).
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Figure 2: eNOS and Akt expressions in quadriceps muscle. Western blots of left (A) and right (B) quadriceps, eNOS (C) and Akt (D) expressions in left quadriceps, eNOS (E) and Akt (F) expressions in left+right quadriceps. Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left+right quadriceps markedly increased in group 3 and 4 (6.4 and 7.7-fold each compared with group 1, 3.5 and 4.2-fold each compared with group 2, p=0.007).

Mentions: The Akt expression in the left quadriceps femoris muscle was shown to be 3.4-fold higher in group 4 than group 2, and 5.3-fold higher in group 4 than group 3 (p=0.026) (Fig. 2). When the eNOS expression in the left and right quadriceps femoris muscles was summed and analyzed, it was shown to be higher in the groups 3 and 4 than in the groups 1 (control) and 2 (p=0.007, Kruskal-Wallis test) (Fig. 2). However, no significant difference in summed Akt expression in the left and right quadriceps femoris muscles and eNOS expression in the left quadriceps femoris muscle was found among the four groups (Fig. 2).


Effect of magnetic stimulation in spinal cord on limb angiogenesis and implication: a pilot study.

Lee D, Beom J, Oh BM, Seo KS - Ann Rehabil Med (2012)

eNOS and Akt expressions in quadriceps muscle. Western blots of left (A) and right (B) quadriceps, eNOS (C) and Akt (D) expressions in left quadriceps, eNOS (E) and Akt (F) expressions in left+right quadriceps. Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left+right quadriceps markedly increased in group 3 and 4 (6.4 and 7.7-fold each compared with group 1, 3.5 and 4.2-fold each compared with group 2, p=0.007).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3400869&req=5

Figure 2: eNOS and Akt expressions in quadriceps muscle. Western blots of left (A) and right (B) quadriceps, eNOS (C) and Akt (D) expressions in left quadriceps, eNOS (E) and Akt (F) expressions in left+right quadriceps. Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left+right quadriceps markedly increased in group 3 and 4 (6.4 and 7.7-fold each compared with group 1, 3.5 and 4.2-fold each compared with group 2, p=0.007).
Mentions: The Akt expression in the left quadriceps femoris muscle was shown to be 3.4-fold higher in group 4 than group 2, and 5.3-fold higher in group 4 than group 3 (p=0.026) (Fig. 2). When the eNOS expression in the left and right quadriceps femoris muscles was summed and analyzed, it was shown to be higher in the groups 3 and 4 than in the groups 1 (control) and 2 (p=0.007, Kruskal-Wallis test) (Fig. 2). However, no significant difference in summed Akt expression in the left and right quadriceps femoris muscles and eNOS expression in the left quadriceps femoris muscle was found among the four groups (Fig. 2).

Bottom Line: Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026).Expression of eNOS in left plus right quadriceps markedly increased in group 3 and 4 compared with group 1 and 2 (p=0.007).Repetitive magnetic stimulation of the spinal cord may exert an angiogenic effect closely linked to lymphangiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul 110-744, Korea.

ABSTRACT

Objective: To investigate the effect of repetitive magnetic stimulation (rMS) of the spinal cord on limb angiogenesis in healthy rats and explore its implication for the treatment of lymphedema.

Method: Twelve adult male Sprague-Dawley rats were divided into four groups as follows: sham rMS followed by tissue harvest 5 minutes later (group 1, n=2), 1 Hz rMS and tissue harvest 5 minutes later (group 2, n=3), 20 Hz rMS and tissue harvest 5 minutes later (group 3, n=3), 20 Hz rMS and tissue harvest 30 minutes later (group 4, n=4). Animals were treated with 20-minute rMS with 120% of the motor threshold on their left side of upper lumbar spinal cord. Expression of angiogenic factors, that is, Akt, phospho-Akt (pAkt), endothelial nitric oxide synthase (eNOS), phospho-eNOS (p-eNOS) were measured by western blot. Bilateral hindlimb muscles (quadriceps and gastrocnemius) were harvested.

Results: Expression of Akt in left quadriceps increased in group 4 compared with group 2 and 3 (3.4 and 5.3-fold each, p=0.026). Expression of eNOS in left plus right quadriceps markedly increased in group 3 and 4 compared with group 1 and 2 (p=0.007). Expressions of eNOS, Akt and p-eNOS, pAkt in gastrocnemius were not comparable between four groups (p>0.05).

Conclusion: Repetitive magnetic stimulation of the spinal cord may exert an angiogenic effect closely linked to lymphangiogenesis. It has clinical implication for the possible therapy of lymphedema caused by breast, cervical or endometrial cancer operation. Future studies with the specific lymphatic endothelial cell markers are required to confirm the effect of rMS on lymphangiogenesis.

No MeSH data available.


Related in: MedlinePlus