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S100B protein expression in the heart of deceased individuals by overdose: a new forensic marker?

Faa A, Senes G, Locci A, Pampaloni P, Pais ME, Piras B, d'Aloja E, Faa G - Clinics (Sao Paulo) (2012)

Bottom Line: The S100B protein was detected in the heart samples of all 15 cases of drug-related deaths; S100B immunoreactivity was mainly observed in the cytoplasm of cardiomyocytes and as globular deposits in the interstitial spaces.No reactivity or weak reactivity was found in the cardiomyocytes of the 59 subjects who died of other causes.Given the near absence of S100B protein in the heart of subjects who died from causes other than drug overdose, S100B immunopositivity may be used as a new ancillary screening tool for the postmortem diagnosis of overdose-related cardiac death.

View Article: PubMed Central - PubMed

Affiliation: University of Cagliari, Department of Pathology, Cagliari, Italy. armando.faa@hotmail.it

ABSTRACT

Objective: The evaluation of S100B protein expression in the human heart and its correlation with drug-related death.

Method: Left ventricular samples were collected from 74 serial forensic autopsies (15 overdose-related deaths; 59 non-overdose-related deaths) from 2007 to 2010. Tissue sections from each sample were immunostained for S100B protein by a commercial antibody.

Results: The S100B protein was detected in the heart samples of all 15 cases of drug-related deaths; S100B immunoreactivity was mainly observed in the cytoplasm of cardiomyocytes and as globular deposits in the interstitial spaces. No reactivity or weak reactivity was found in the cardiomyocytes of the 59 subjects who died of other causes.

Conclusion: Our preliminary data show that the S100B protein accumulates in injured cardiomyocytes during drug-related sudden death. Given the near absence of S100B protein in the heart of subjects who died from causes other than drug overdose, S100B immunopositivity may be used as a new ancillary screening tool for the postmortem diagnosis of overdose-related cardiac death.

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S100B positivity in inter-myocytes spaces with accentuation of Schwann cells in a nerve twig X 400.
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f3-cln_67p821: S100B positivity in inter-myocytes spaces with accentuation of Schwann cells in a nerve twig X 400.

Mentions: Immunoreactivity for the S100B protein was mainly detected in the overdose group; less reactivity was detected in the 59 subjects in the non-overdose-related group (Figure 1). The pattern of S100B reactivity varied between cases, with different localization and different intensities. S100B staining was mainly observed inside the cytoplasm of scattered cardiac cells (Figure 2). The signals appeared as differently sized globular deposits and granules, often located near the nucleus. Similar globular deposits were also found in the interstitial spaces and were unevenly distributed throughout the cardiac tissue (Figure 3). Immunoreactive signals were also occasionally found in the perivascular spaces in the cytoplasm of stromal cells surrounding the wall of intra-cardiac arteries. No significant differences were found among the different subgroups in which the overdose patients were subdivided: S100B protein deposits were found in the heart in cocaine-, heroin- and other drug-related deaths. We observed the highest levels of immunoreactivity for S100B in 3 patients with a clinical history of combined cocaine and heroin abuse (Figure 4). In these cases, a large number of cardiomyocytes showed very large globular deposits of the protein.


S100B protein expression in the heart of deceased individuals by overdose: a new forensic marker?

Faa A, Senes G, Locci A, Pampaloni P, Pais ME, Piras B, d'Aloja E, Faa G - Clinics (Sao Paulo) (2012)

S100B positivity in inter-myocytes spaces with accentuation of Schwann cells in a nerve twig X 400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3400175&req=5

f3-cln_67p821: S100B positivity in inter-myocytes spaces with accentuation of Schwann cells in a nerve twig X 400.
Mentions: Immunoreactivity for the S100B protein was mainly detected in the overdose group; less reactivity was detected in the 59 subjects in the non-overdose-related group (Figure 1). The pattern of S100B reactivity varied between cases, with different localization and different intensities. S100B staining was mainly observed inside the cytoplasm of scattered cardiac cells (Figure 2). The signals appeared as differently sized globular deposits and granules, often located near the nucleus. Similar globular deposits were also found in the interstitial spaces and were unevenly distributed throughout the cardiac tissue (Figure 3). Immunoreactive signals were also occasionally found in the perivascular spaces in the cytoplasm of stromal cells surrounding the wall of intra-cardiac arteries. No significant differences were found among the different subgroups in which the overdose patients were subdivided: S100B protein deposits were found in the heart in cocaine-, heroin- and other drug-related deaths. We observed the highest levels of immunoreactivity for S100B in 3 patients with a clinical history of combined cocaine and heroin abuse (Figure 4). In these cases, a large number of cardiomyocytes showed very large globular deposits of the protein.

Bottom Line: The S100B protein was detected in the heart samples of all 15 cases of drug-related deaths; S100B immunoreactivity was mainly observed in the cytoplasm of cardiomyocytes and as globular deposits in the interstitial spaces.No reactivity or weak reactivity was found in the cardiomyocytes of the 59 subjects who died of other causes.Given the near absence of S100B protein in the heart of subjects who died from causes other than drug overdose, S100B immunopositivity may be used as a new ancillary screening tool for the postmortem diagnosis of overdose-related cardiac death.

View Article: PubMed Central - PubMed

Affiliation: University of Cagliari, Department of Pathology, Cagliari, Italy. armando.faa@hotmail.it

ABSTRACT

Objective: The evaluation of S100B protein expression in the human heart and its correlation with drug-related death.

Method: Left ventricular samples were collected from 74 serial forensic autopsies (15 overdose-related deaths; 59 non-overdose-related deaths) from 2007 to 2010. Tissue sections from each sample were immunostained for S100B protein by a commercial antibody.

Results: The S100B protein was detected in the heart samples of all 15 cases of drug-related deaths; S100B immunoreactivity was mainly observed in the cytoplasm of cardiomyocytes and as globular deposits in the interstitial spaces. No reactivity or weak reactivity was found in the cardiomyocytes of the 59 subjects who died of other causes.

Conclusion: Our preliminary data show that the S100B protein accumulates in injured cardiomyocytes during drug-related sudden death. Given the near absence of S100B protein in the heart of subjects who died from causes other than drug overdose, S100B immunopositivity may be used as a new ancillary screening tool for the postmortem diagnosis of overdose-related cardiac death.

Show MeSH
Related in: MedlinePlus