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Time-related effects of general functional training in spinal cord-injured rats.

Miranda TA, Vicente JM, Marcon RM, Cristante AF, Morya E, Valle AC - Clinics (Sao Paulo) (2012)

Bottom Line: Ramp and maze groups showed an earlier and greater functional improvement effect than the control and runway groups.However, over time, unexpectedly, all of the groups showed similar effects as the control group, with spontaneous recovery.Short-term benefits can be associated with a specific training regime; however, the same training was ineffective at maintaining superior long-term recovery.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina da Universidade de São Paulo, Program of Experimental Physiopathology, São Paulo/SP, Brazil. taisaabm@hotmail.com

ABSTRACT

Objectives: This prospective, randomized, experimental study with rats aimed to investigate the influence of general treatment strategies on the motor recovery of Wistar rats with moderate contusive spinal cord injury.

Methods: A total of 51 Wistar rats were randomized into five groups: control, maze, ramp, runway, and sham (laminectomy only). The rats underwent spinal cord injury at the T9-T10 levels using the NYU-Impactor. Each group was trained for 12 minutes twice a week for two weeks before and five weeks after the spinal cord injury, except for the control group. Functional motor recovery was assessed with the Basso, Beattie, and Bresnahan Scale on the first postoperative day and then once a week for five weeks. The animals were euthanized, and the spinal cords were collected for histological analysis.

Results: Ramp and maze groups showed an earlier and greater functional improvement effect than the control and runway groups. However, over time, unexpectedly, all of the groups showed similar effects as the control group, with spontaneous recovery. There were no histological differences in the injured area between the trained and control groups.

Conclusion: Short-term benefits can be associated with a specific training regime; however, the same training was ineffective at maintaining superior long-term recovery. These results might support new considerations before hospital discharge of patients with spinal cord injuries.

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Related in: MedlinePlus

The time course of locomotor recovery as measured by BBB open field scores for ramp, maze, runway control and sham groups. Since the 1st POd the sham group punctuated 21, showing no motor deficits. Maze and ramp groups showed greatest recovery when compared to runway and control groups on the 8th POd (*p  =  0.001), but this difference did not persist till the 36th POd, although the ramp group tended to have a better performance.
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f3-cln_67p799: The time course of locomotor recovery as measured by BBB open field scores for ramp, maze, runway control and sham groups. Since the 1st POd the sham group punctuated 21, showing no motor deficits. Maze and ramp groups showed greatest recovery when compared to runway and control groups on the 8th POd (*p  =  0.001), but this difference did not persist till the 36th POd, although the ramp group tended to have a better performance.

Mentions: As expected, the sham group achieved the highest BBB score (21 points) from the first day after the surgical procedure without spinal cord injury. The other groups showed no significant differences on the 1st POd (F3,30 = 0.34, p = 0.790, Figure 3), indicating a contusive injury consistency with no movements or slight movement of one or two hindlimb joints (mean locomotor BBB scale ranging between 0 to 0.4).


Time-related effects of general functional training in spinal cord-injured rats.

Miranda TA, Vicente JM, Marcon RM, Cristante AF, Morya E, Valle AC - Clinics (Sao Paulo) (2012)

The time course of locomotor recovery as measured by BBB open field scores for ramp, maze, runway control and sham groups. Since the 1st POd the sham group punctuated 21, showing no motor deficits. Maze and ramp groups showed greatest recovery when compared to runway and control groups on the 8th POd (*p  =  0.001), but this difference did not persist till the 36th POd, although the ramp group tended to have a better performance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3400172&req=5

f3-cln_67p799: The time course of locomotor recovery as measured by BBB open field scores for ramp, maze, runway control and sham groups. Since the 1st POd the sham group punctuated 21, showing no motor deficits. Maze and ramp groups showed greatest recovery when compared to runway and control groups on the 8th POd (*p  =  0.001), but this difference did not persist till the 36th POd, although the ramp group tended to have a better performance.
Mentions: As expected, the sham group achieved the highest BBB score (21 points) from the first day after the surgical procedure without spinal cord injury. The other groups showed no significant differences on the 1st POd (F3,30 = 0.34, p = 0.790, Figure 3), indicating a contusive injury consistency with no movements or slight movement of one or two hindlimb joints (mean locomotor BBB scale ranging between 0 to 0.4).

Bottom Line: Ramp and maze groups showed an earlier and greater functional improvement effect than the control and runway groups.However, over time, unexpectedly, all of the groups showed similar effects as the control group, with spontaneous recovery.Short-term benefits can be associated with a specific training regime; however, the same training was ineffective at maintaining superior long-term recovery.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina da Universidade de São Paulo, Program of Experimental Physiopathology, São Paulo/SP, Brazil. taisaabm@hotmail.com

ABSTRACT

Objectives: This prospective, randomized, experimental study with rats aimed to investigate the influence of general treatment strategies on the motor recovery of Wistar rats with moderate contusive spinal cord injury.

Methods: A total of 51 Wistar rats were randomized into five groups: control, maze, ramp, runway, and sham (laminectomy only). The rats underwent spinal cord injury at the T9-T10 levels using the NYU-Impactor. Each group was trained for 12 minutes twice a week for two weeks before and five weeks after the spinal cord injury, except for the control group. Functional motor recovery was assessed with the Basso, Beattie, and Bresnahan Scale on the first postoperative day and then once a week for five weeks. The animals were euthanized, and the spinal cords were collected for histological analysis.

Results: Ramp and maze groups showed an earlier and greater functional improvement effect than the control and runway groups. However, over time, unexpectedly, all of the groups showed similar effects as the control group, with spontaneous recovery. There were no histological differences in the injured area between the trained and control groups.

Conclusion: Short-term benefits can be associated with a specific training regime; however, the same training was ineffective at maintaining superior long-term recovery. These results might support new considerations before hospital discharge of patients with spinal cord injuries.

Show MeSH
Related in: MedlinePlus