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The use of vancomycin-loaded poly-l-lactic acid and poly-ethylene oxide microspheres for bone repair: an in vivo study.

Coraca-Huber DC, Duek EA, Etchebehere M, Magna LA, Amstalden EM - Clinics (Sao Paulo) (2012)

Bottom Line: The groups implanted with vancomycin-loaded and vancomycin-unloaded microspheres were compared.Woven bone formation with progressive maturation was observed.Local antibiotic therapy using vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres should be considered after the microbiological evaluation of its efficacy.

View Article: PubMed Central - PubMed

Affiliation: Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University Innsbruck, Austria. debora.coraca-huber@i-med.ac.at

ABSTRACT

Objective: The aim of this study was to investigate bone repair after the implantation of vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres compared with vancomycin-unloaded poly-L-lactic acid/poly-ethylene oxide microspheres.

Methods: Poly-L-lactic acid/poly-ethylene oxide microspheres were implanted in rat tibiae and evaluated for periods of 2, 4, 8, and 12 days and 4, 8, 16, and 32 weeks. The groups implanted with vancomycin-loaded and vancomycin-unloaded microspheres were compared. Histopathologic (semi-quantitative) and histomorphometric analyses were performed to evaluate the bone formation process.

Results: During the first period (second day), fibrin and hemorrhaging areas were observed to be replaced by granulation tissue around the microspheres. Woven bone formation with progressive maturation was observed. All of the histopathological findings, evaluated by a semi-quantitative assay and a quantitative analysis (percentage of bone formation), were similar between the two groups.

Conclusion: Vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres are a good bone substitute candidate for bone repair. Local antibiotic therapy using vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres should be considered after the microbiological evaluation of its efficacy.

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Histology of tibias implanted with PLLA/PEO that were A) vancomycin-loaded or B) vancomycin-unloaded after 4 weeks, showing thin trabecular bone (arrow); and PLLA/PEO that were C) vancomycin-loaded or D) vancomycin-unloaded during a 32-week period, showing massive bone formation surrounding the microspheres with evident maturation and the presence of lamellar bone (arrow) (Original H&E 10×).
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f3-cln_67p793: Histology of tibias implanted with PLLA/PEO that were A) vancomycin-loaded or B) vancomycin-unloaded after 4 weeks, showing thin trabecular bone (arrow); and PLLA/PEO that were C) vancomycin-loaded or D) vancomycin-unloaded during a 32-week period, showing massive bone formation surrounding the microspheres with evident maturation and the presence of lamellar bone (arrow) (Original H&E 10×).

Mentions: The microscopic findings were similar in both groups throughout the experimental periods. The vancomycin-loaded and vancomycin-unloaded PLLA/PEO microspheres were easily visible inside the medullary cavity in both groups during all evaluated periods (Figures 1, 2, and 3). After two days, fibrin and hemorrhaging were visibly close to the implant in the medullary cavity and were replaced by granulation tissue on approximately the fourth day (Figure 1A).


The use of vancomycin-loaded poly-l-lactic acid and poly-ethylene oxide microspheres for bone repair: an in vivo study.

Coraca-Huber DC, Duek EA, Etchebehere M, Magna LA, Amstalden EM - Clinics (Sao Paulo) (2012)

Histology of tibias implanted with PLLA/PEO that were A) vancomycin-loaded or B) vancomycin-unloaded after 4 weeks, showing thin trabecular bone (arrow); and PLLA/PEO that were C) vancomycin-loaded or D) vancomycin-unloaded during a 32-week period, showing massive bone formation surrounding the microspheres with evident maturation and the presence of lamellar bone (arrow) (Original H&E 10×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3400171&req=5

f3-cln_67p793: Histology of tibias implanted with PLLA/PEO that were A) vancomycin-loaded or B) vancomycin-unloaded after 4 weeks, showing thin trabecular bone (arrow); and PLLA/PEO that were C) vancomycin-loaded or D) vancomycin-unloaded during a 32-week period, showing massive bone formation surrounding the microspheres with evident maturation and the presence of lamellar bone (arrow) (Original H&E 10×).
Mentions: The microscopic findings were similar in both groups throughout the experimental periods. The vancomycin-loaded and vancomycin-unloaded PLLA/PEO microspheres were easily visible inside the medullary cavity in both groups during all evaluated periods (Figures 1, 2, and 3). After two days, fibrin and hemorrhaging were visibly close to the implant in the medullary cavity and were replaced by granulation tissue on approximately the fourth day (Figure 1A).

Bottom Line: The groups implanted with vancomycin-loaded and vancomycin-unloaded microspheres were compared.Woven bone formation with progressive maturation was observed.Local antibiotic therapy using vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres should be considered after the microbiological evaluation of its efficacy.

View Article: PubMed Central - PubMed

Affiliation: Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University Innsbruck, Austria. debora.coraca-huber@i-med.ac.at

ABSTRACT

Objective: The aim of this study was to investigate bone repair after the implantation of vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres compared with vancomycin-unloaded poly-L-lactic acid/poly-ethylene oxide microspheres.

Methods: Poly-L-lactic acid/poly-ethylene oxide microspheres were implanted in rat tibiae and evaluated for periods of 2, 4, 8, and 12 days and 4, 8, 16, and 32 weeks. The groups implanted with vancomycin-loaded and vancomycin-unloaded microspheres were compared. Histopathologic (semi-quantitative) and histomorphometric analyses were performed to evaluate the bone formation process.

Results: During the first period (second day), fibrin and hemorrhaging areas were observed to be replaced by granulation tissue around the microspheres. Woven bone formation with progressive maturation was observed. All of the histopathological findings, evaluated by a semi-quantitative assay and a quantitative analysis (percentage of bone formation), were similar between the two groups.

Conclusion: Vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres are a good bone substitute candidate for bone repair. Local antibiotic therapy using vancomycin-loaded poly-L-lactic acid/poly-ethylene oxide microspheres should be considered after the microbiological evaluation of its efficacy.

Show MeSH