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Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E.

Al-Damegh MA - Clinics (Sao Paulo) (2012)

Bottom Line: There was a significant increase in the diameter of the seminiferous tubules with a disorganized seminiferous tubule sperm cycle interruption in the electromagnetism-exposed group.Our results indicate that the adverse effect of the generated electromagnetic frequency had a negative impact on testicular architecture and enzymatic activity.This finding also indicated the possible role of vitamins C and E in mitigating the oxidative stress imposed on the testes and restoring normality to the testes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, College of Science and Arts, Onaizah, Qassim University, Kingdom of Saudi Arabia. dr_mona_aldamegh@yahoo.com

ABSTRACT

Objective: The aim of this study was to investigate the possible effects of electromagnetic radiation from conventional cellular phone use on the oxidant and antioxidant status in rat blood and testicular tissue and determine the possible protective role of vitamins C and E in preventing the detrimental effects of electromagnetic radiation on the testes.

Materials and methods: The treatment groups were exposed to an electromagnetic field, electromagnetic field plus vitamin C (40 mg/kg/day) or electromagnetic field plus vitamin E (2.7 mg/kg/day). All groups were exposed to the same electromagnetic frequency for 15, 30, and 60 min daily for two weeks.

Results: There was a significant increase in the diameter of the seminiferous tubules with a disorganized seminiferous tubule sperm cycle interruption in the electromagnetism-exposed group. The serum and testicular tissue conjugated diene, lipid hydroperoxide, and catalase activities increased 3-fold, whereas the total serum and testicular tissue glutathione and glutathione peroxidase levels decreased 3-5 fold in the electromagnetism-exposed animals.

Conclusion: Our results indicate that the adverse effect of the generated electromagnetic frequency had a negative impact on testicular architecture and enzymatic activity. This finding also indicated the possible role of vitamins C and E in mitigating the oxidative stress imposed on the testes and restoring normality to the testes.

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Related in: MedlinePlus

Section of rat testis showing the normal seminiferous tubules, interstitium, spermatids and spermatogenic cells at different stages of development. H&E ×400.
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f1-cln_67p785: Section of rat testis showing the normal seminiferous tubules, interstitium, spermatids and spermatogenic cells at different stages of development. H&E ×400.

Mentions: The testis sections in the three control groups revealed that the seminiferous tubules consist of several layers of epithelial cells (Figure 1). The cuboidal spermatogonia cells have clear cytoplasm and rounded nuclei, which may show division. Sertoli cells are large epithelial cells that have a vesicular nucleus with a large nucleolus. Next to the spermatogonia cells is a zone of spermatocytes, the nuclei of which are usually in mitotic division. A large number of small cells, the spermatids, are seen external to the spermatocytes. The spermatids then further develop into spermatozoa. The latter usually lie in groups with their heads projecting between the deeper cells and are connected with one of the Sertoli cells of the lining epithelium.


Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E.

Al-Damegh MA - Clinics (Sao Paulo) (2012)

Section of rat testis showing the normal seminiferous tubules, interstitium, spermatids and spermatogenic cells at different stages of development. H&E ×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3400170&req=5

f1-cln_67p785: Section of rat testis showing the normal seminiferous tubules, interstitium, spermatids and spermatogenic cells at different stages of development. H&E ×400.
Mentions: The testis sections in the three control groups revealed that the seminiferous tubules consist of several layers of epithelial cells (Figure 1). The cuboidal spermatogonia cells have clear cytoplasm and rounded nuclei, which may show division. Sertoli cells are large epithelial cells that have a vesicular nucleus with a large nucleolus. Next to the spermatogonia cells is a zone of spermatocytes, the nuclei of which are usually in mitotic division. A large number of small cells, the spermatids, are seen external to the spermatocytes. The spermatids then further develop into spermatozoa. The latter usually lie in groups with their heads projecting between the deeper cells and are connected with one of the Sertoli cells of the lining epithelium.

Bottom Line: There was a significant increase in the diameter of the seminiferous tubules with a disorganized seminiferous tubule sperm cycle interruption in the electromagnetism-exposed group.Our results indicate that the adverse effect of the generated electromagnetic frequency had a negative impact on testicular architecture and enzymatic activity.This finding also indicated the possible role of vitamins C and E in mitigating the oxidative stress imposed on the testes and restoring normality to the testes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, College of Science and Arts, Onaizah, Qassim University, Kingdom of Saudi Arabia. dr_mona_aldamegh@yahoo.com

ABSTRACT

Objective: The aim of this study was to investigate the possible effects of electromagnetic radiation from conventional cellular phone use on the oxidant and antioxidant status in rat blood and testicular tissue and determine the possible protective role of vitamins C and E in preventing the detrimental effects of electromagnetic radiation on the testes.

Materials and methods: The treatment groups were exposed to an electromagnetic field, electromagnetic field plus vitamin C (40 mg/kg/day) or electromagnetic field plus vitamin E (2.7 mg/kg/day). All groups were exposed to the same electromagnetic frequency for 15, 30, and 60 min daily for two weeks.

Results: There was a significant increase in the diameter of the seminiferous tubules with a disorganized seminiferous tubule sperm cycle interruption in the electromagnetism-exposed group. The serum and testicular tissue conjugated diene, lipid hydroperoxide, and catalase activities increased 3-fold, whereas the total serum and testicular tissue glutathione and glutathione peroxidase levels decreased 3-5 fold in the electromagnetism-exposed animals.

Conclusion: Our results indicate that the adverse effect of the generated electromagnetic frequency had a negative impact on testicular architecture and enzymatic activity. This finding also indicated the possible role of vitamins C and E in mitigating the oxidative stress imposed on the testes and restoring normality to the testes.

Show MeSH
Related in: MedlinePlus