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A piezoelectric immunosensor using hybrid self-assembled monolayers for detection of Schistosoma japonicum.

Wang S, Yin T, Zeng S, Che H, Yang F, Chen X, Shen G, Wu Z - PLoS ONE (2012)

Bottom Line: The correlation coefficients reached 0.973.We have developed a new, simple, sensitive, and reusable piezoelectric immunosensor that directly detects SjAb in the serum.This method may represent an alternative to the current diagnostic methods for S. japonicum infection in the clinical laboratory or for analysis outside the laboratory.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Schistosomiasis in Hunan, Department of Parasitology, Xiangya Medical College, Central South University, Changsha, China. spwang@126.com

ABSTRACT

Background: The parasite Schistosoma japonicum causes schistosomiasis disease, which threatens human life and hampers economic and social development in some Asian countries. An important lesson learned from efforts to reduce the occurrence of schistosomiasis is that the diagnostic approach must be altered as further progress is made towards the control and ultimate elimination of the disease.

Methodology/principal findings: Using mixed self-assembled monolayer membrane (mixed SAM) technology, a mixture of mercaptopropionic acid (MPA) and mercaptoethanol (ME) was self-assembled on the surface of quartz crystals by gold-sulphur-bonds. Soluble egg antigens (SEA) of S. japonicum were then cross-linked to the quartz crystal using a special coupling agent. As compared with the traditional single self-assembled monolayer immobilization method, S. japonicum antigen (SjAg) immobilization using mixed self-assembled monolayers exhibits much greater immunoreactivity. Under optimal experimental conditions, the detection range is 1:1500 to 1:60 (infected rabbit serum dilution ratios). We measured several infected rabbit serum samples with varying S. japonicum antibody (SjAb) concentrations using both immunosensor and ELISA techniques and then produced a correlation analysis. The correlation coefficients reached 0.973.

Conclusions/significance: We have developed a new, simple, sensitive, and reusable piezoelectric immunosensor that directly detects SjAb in the serum. This method may represent an alternative to the current diagnostic methods for S. japonicum infection in the clinical laboratory or for analysis outside the laboratory.

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Effect of SjAg concentration on SjAg immobilization.The MPA and ME of volume ratios in mixed SAM are 3∶7. The SjAg dilution ratio is 1∶100. The best concentration of SjAg for immobilization is 0.2 mg ml−1.
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pone-0030779-g002: Effect of SjAg concentration on SjAg immobilization.The MPA and ME of volume ratios in mixed SAM are 3∶7. The SjAg dilution ratio is 1∶100. The best concentration of SjAg for immobilization is 0.2 mg ml−1.

Mentions: The impact of the SjAg concentration (dilution ratio) on the immobilization of SjAg was evaluated in our experiments. The best immobilization concentration (dilution ratio) of SjAg is 0.2 mg ml−1, which results in the greatest frequency shift response value of the sensor (Figure 2). The frequency shift value decreases gradually with this concentration. As the active sites for SjAg immobilization on the sensor surface is limited, SjAg molecules in the mixed membrane reach a state of equilibrium when the concentration of SjAg increases to a certain value. However, when it exceeds this value, SjAg molecules are in disorder and the steric hindrance with SjAb increased. Therefore, the best concentration of SjAg was selected as 0.2 mg ml−1 for this experiment. In addition, the appropriate temperature conducive for improving the antigen-immobilization speed was 37°C for this experiment.


A piezoelectric immunosensor using hybrid self-assembled monolayers for detection of Schistosoma japonicum.

Wang S, Yin T, Zeng S, Che H, Yang F, Chen X, Shen G, Wu Z - PLoS ONE (2012)

Effect of SjAg concentration on SjAg immobilization.The MPA and ME of volume ratios in mixed SAM are 3∶7. The SjAg dilution ratio is 1∶100. The best concentration of SjAg for immobilization is 0.2 mg ml−1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3383785&req=5

pone-0030779-g002: Effect of SjAg concentration on SjAg immobilization.The MPA and ME of volume ratios in mixed SAM are 3∶7. The SjAg dilution ratio is 1∶100. The best concentration of SjAg for immobilization is 0.2 mg ml−1.
Mentions: The impact of the SjAg concentration (dilution ratio) on the immobilization of SjAg was evaluated in our experiments. The best immobilization concentration (dilution ratio) of SjAg is 0.2 mg ml−1, which results in the greatest frequency shift response value of the sensor (Figure 2). The frequency shift value decreases gradually with this concentration. As the active sites for SjAg immobilization on the sensor surface is limited, SjAg molecules in the mixed membrane reach a state of equilibrium when the concentration of SjAg increases to a certain value. However, when it exceeds this value, SjAg molecules are in disorder and the steric hindrance with SjAb increased. Therefore, the best concentration of SjAg was selected as 0.2 mg ml−1 for this experiment. In addition, the appropriate temperature conducive for improving the antigen-immobilization speed was 37°C for this experiment.

Bottom Line: The correlation coefficients reached 0.973.We have developed a new, simple, sensitive, and reusable piezoelectric immunosensor that directly detects SjAb in the serum.This method may represent an alternative to the current diagnostic methods for S. japonicum infection in the clinical laboratory or for analysis outside the laboratory.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Schistosomiasis in Hunan, Department of Parasitology, Xiangya Medical College, Central South University, Changsha, China. spwang@126.com

ABSTRACT

Background: The parasite Schistosoma japonicum causes schistosomiasis disease, which threatens human life and hampers economic and social development in some Asian countries. An important lesson learned from efforts to reduce the occurrence of schistosomiasis is that the diagnostic approach must be altered as further progress is made towards the control and ultimate elimination of the disease.

Methodology/principal findings: Using mixed self-assembled monolayer membrane (mixed SAM) technology, a mixture of mercaptopropionic acid (MPA) and mercaptoethanol (ME) was self-assembled on the surface of quartz crystals by gold-sulphur-bonds. Soluble egg antigens (SEA) of S. japonicum were then cross-linked to the quartz crystal using a special coupling agent. As compared with the traditional single self-assembled monolayer immobilization method, S. japonicum antigen (SjAg) immobilization using mixed self-assembled monolayers exhibits much greater immunoreactivity. Under optimal experimental conditions, the detection range is 1:1500 to 1:60 (infected rabbit serum dilution ratios). We measured several infected rabbit serum samples with varying S. japonicum antibody (SjAb) concentrations using both immunosensor and ELISA techniques and then produced a correlation analysis. The correlation coefficients reached 0.973.

Conclusions/significance: We have developed a new, simple, sensitive, and reusable piezoelectric immunosensor that directly detects SjAb in the serum. This method may represent an alternative to the current diagnostic methods for S. japonicum infection in the clinical laboratory or for analysis outside the laboratory.

Show MeSH
Related in: MedlinePlus