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Effects of the insemination of hydrogen peroxide-treated epididymal mouse spermatozoa on γH2AX repair and embryo development.

Xiao J, Liu Y, Li Z, Zhou Y, Lin H, Wu X, Chen M, Xiao W - PLoS ONE (2012)

Bottom Line: We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm.Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage.Immunocytochemistry revealed γH2AX in the one- and four-cell embryos. γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, People's Republic of China.

ABSTRACT

Background: Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused by excessive reactive oxygen species (ROS) generation.

Methods and findings: We used exogenous ROS (H(2)O(2)) to simulate cryopreservation and examined DNA damage repair in embryos fertilized with sperm with H(2)O(2)-induced DNA damage. Sperm samples were collected from epididymis of adult male KM mice and treated with capacitation medium (containing 0, 0.1, 0.5 and 1 mM H(2)O(2)) or cryopreservation. The model of DNA-damaged sperm was based on sperm motility, viability and the expression of γH2AX, the DNA damage-repair marker. We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm. Cryopreservation and 1-mM H(2)O(2) treatment produced similar DNA damage. Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage. Immunocytochemistry revealed γH2AX in the one- and four-cell embryos.

Conclusions: γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa.

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Related in: MedlinePlus

Delay in cleavage of one- and two-celled embryos after fertilization.Sperm was treated with 1 mM H2O2 and the time of embryo cleavage was examined. A: Cleavage rate of one- to two-celled embryos and B: two- to four-celled embryos.
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pone-0038742-g003: Delay in cleavage of one- and two-celled embryos after fertilization.Sperm was treated with 1 mM H2O2 and the time of embryo cleavage was examined. A: Cleavage rate of one- to two-celled embryos and B: two- to four-celled embryos.

Mentions: At 32 h after insemination, 4% of four-celled embryos had cleaved with fresh spermatozoa. However, no embryos had cleaved with sperm treated with 1 mM H2O2. At 36 h, the percentage cleaved embryos was 11% and 7% for fresh spermatozoa and sperm treated with 1 mM H2O2, respectively, and at 48 hr, the percentage cleaved peaked at 84% and 80%, respectively. Thus, cleavage of one- and two-celled embryos was delayed in part in sperm treated with 1 mM H2O2 as compared with fresh spermatozoa after insemination (Fig. 3).


Effects of the insemination of hydrogen peroxide-treated epididymal mouse spermatozoa on γH2AX repair and embryo development.

Xiao J, Liu Y, Li Z, Zhou Y, Lin H, Wu X, Chen M, Xiao W - PLoS ONE (2012)

Delay in cleavage of one- and two-celled embryos after fertilization.Sperm was treated with 1 mM H2O2 and the time of embryo cleavage was examined. A: Cleavage rate of one- to two-celled embryos and B: two- to four-celled embryos.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3383764&req=5

pone-0038742-g003: Delay in cleavage of one- and two-celled embryos after fertilization.Sperm was treated with 1 mM H2O2 and the time of embryo cleavage was examined. A: Cleavage rate of one- to two-celled embryos and B: two- to four-celled embryos.
Mentions: At 32 h after insemination, 4% of four-celled embryos had cleaved with fresh spermatozoa. However, no embryos had cleaved with sperm treated with 1 mM H2O2. At 36 h, the percentage cleaved embryos was 11% and 7% for fresh spermatozoa and sperm treated with 1 mM H2O2, respectively, and at 48 hr, the percentage cleaved peaked at 84% and 80%, respectively. Thus, cleavage of one- and two-celled embryos was delayed in part in sperm treated with 1 mM H2O2 as compared with fresh spermatozoa after insemination (Fig. 3).

Bottom Line: We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm.Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage.Immunocytochemistry revealed γH2AX in the one- and four-cell embryos. γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, People's Republic of China.

ABSTRACT

Background: Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused by excessive reactive oxygen species (ROS) generation.

Methods and findings: We used exogenous ROS (H(2)O(2)) to simulate cryopreservation and examined DNA damage repair in embryos fertilized with sperm with H(2)O(2)-induced DNA damage. Sperm samples were collected from epididymis of adult male KM mice and treated with capacitation medium (containing 0, 0.1, 0.5 and 1 mM H(2)O(2)) or cryopreservation. The model of DNA-damaged sperm was based on sperm motility, viability and the expression of γH2AX, the DNA damage-repair marker. We examined fertility rate, development, cell cleavage, and γH2AX level in embryos fertilized with DNA-damaged sperm. Cryopreservation and 1-mM H(2)O(2) treatment produced similar DNA damage. Most of the one- and two-cell embryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage. Immunocytochemistry revealed γH2AX in the one- and four-cell embryos.

Conclusions: γH2AX may be involved in repair of preimplantation embryos fertilized with oxygen-stressed spermatozoa.

Show MeSH
Related in: MedlinePlus