Limits...
Tumor cell and carcinoma-associated fibroblast interaction regulates matrix metalloproteinases and their inhibitors in oral squamous cell carcinoma.

Fullár A, Kovalszky I, Bitsche M, Romani A, Schartinger VH, Sprinzl GM, Riechelmann H, Dudás J - Exp. Cell Res. (2012)

Bottom Line: In addition, these cells also cooperated in activation of MMP pro-enzymes.It is particularly interesting that the fibroblast-produced inactive MMP-2 has been activated by the tumor-cell-produced membrane-type 1 matrix metalloproteinase (MT1-MMP).The crosstalk between cancer- and the surrounding fibroblast stromal-cells is essential for the fine tuning of cancer cells invasivity.

View Article: PubMed Central - PubMed

Affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary. fullarsz@gmail.com

Show MeSH

Related in: MedlinePlus

Detection of MMP-2 and MMP-9 gelatinase activity in cell lysate (A) and supernatant (B) of fibroblasts and SCC-25 cells by combined gelatinase zymography. 1–2: fibroblast control, 3–4: SCC-25 control, 5–6: fibroblast co-culture, 7–8: SCC-25 co-culture. Densitometry of MMP-2 and MMP-9 gelatinase zymography (C–F). C and D represent the densitometry of pro-MMP-9 (C) and active MMP-9 (D) bands in cell lysates. E and F show the densitometry of pro-MMP-2 (E) and active MMP-2 (F) form from supernatants.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3378977&req=5

f0035: Detection of MMP-2 and MMP-9 gelatinase activity in cell lysate (A) and supernatant (B) of fibroblasts and SCC-25 cells by combined gelatinase zymography. 1–2: fibroblast control, 3–4: SCC-25 control, 5–6: fibroblast co-culture, 7–8: SCC-25 co-culture. Densitometry of MMP-2 and MMP-9 gelatinase zymography (C–F). C and D represent the densitometry of pro-MMP-9 (C) and active MMP-9 (D) bands in cell lysates. E and F show the densitometry of pro-MMP-2 (E) and active MMP-2 (F) form from supernatants.

Mentions: MMP-2 and -9 were functionally analysed in gelatinase zymography. Both supernatants and cell lysates of controls and co-cultures (of both the SCC-25- and PDL-side) were used in this assay. Active and inactive bands of MMP-9 and MMP-2 were recognized, and active bands of these enzymes were found at lower molecular weight. 5 μl of fetal bovine serum was used as positive control (Figs. 6A–B, FBS lane).


Tumor cell and carcinoma-associated fibroblast interaction regulates matrix metalloproteinases and their inhibitors in oral squamous cell carcinoma.

Fullár A, Kovalszky I, Bitsche M, Romani A, Schartinger VH, Sprinzl GM, Riechelmann H, Dudás J - Exp. Cell Res. (2012)

Detection of MMP-2 and MMP-9 gelatinase activity in cell lysate (A) and supernatant (B) of fibroblasts and SCC-25 cells by combined gelatinase zymography. 1–2: fibroblast control, 3–4: SCC-25 control, 5–6: fibroblast co-culture, 7–8: SCC-25 co-culture. Densitometry of MMP-2 and MMP-9 gelatinase zymography (C–F). C and D represent the densitometry of pro-MMP-9 (C) and active MMP-9 (D) bands in cell lysates. E and F show the densitometry of pro-MMP-2 (E) and active MMP-2 (F) form from supernatants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378977&req=5

f0035: Detection of MMP-2 and MMP-9 gelatinase activity in cell lysate (A) and supernatant (B) of fibroblasts and SCC-25 cells by combined gelatinase zymography. 1–2: fibroblast control, 3–4: SCC-25 control, 5–6: fibroblast co-culture, 7–8: SCC-25 co-culture. Densitometry of MMP-2 and MMP-9 gelatinase zymography (C–F). C and D represent the densitometry of pro-MMP-9 (C) and active MMP-9 (D) bands in cell lysates. E and F show the densitometry of pro-MMP-2 (E) and active MMP-2 (F) form from supernatants.
Mentions: MMP-2 and -9 were functionally analysed in gelatinase zymography. Both supernatants and cell lysates of controls and co-cultures (of both the SCC-25- and PDL-side) were used in this assay. Active and inactive bands of MMP-9 and MMP-2 were recognized, and active bands of these enzymes were found at lower molecular weight. 5 μl of fetal bovine serum was used as positive control (Figs. 6A–B, FBS lane).

Bottom Line: In addition, these cells also cooperated in activation of MMP pro-enzymes.It is particularly interesting that the fibroblast-produced inactive MMP-2 has been activated by the tumor-cell-produced membrane-type 1 matrix metalloproteinase (MT1-MMP).The crosstalk between cancer- and the surrounding fibroblast stromal-cells is essential for the fine tuning of cancer cells invasivity.

View Article: PubMed Central - PubMed

Affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary. fullarsz@gmail.com

Show MeSH
Related in: MedlinePlus