Tumor cell and carcinoma-associated fibroblast interaction regulates matrix metalloproteinases and their inhibitors in oral squamous cell carcinoma.
Bottom Line: In addition, these cells also cooperated in activation of MMP pro-enzymes.It is particularly interesting that the fibroblast-produced inactive MMP-2 has been activated by the tumor-cell-produced membrane-type 1 matrix metalloproteinase (MT1-MMP).The crosstalk between cancer- and the surrounding fibroblast stromal-cells is essential for the fine tuning of cancer cells invasivity.
Affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: MMP-2 and -9 were functionally analysed in gelatinase zymography. Both supernatants and cell lysates of controls and co-cultures (of both the SCC-25- and PDL-side) were used in this assay. Active and inactive bands of MMP-9 and MMP-2 were recognized, and active bands of these enzymes were found at lower molecular weight. 5 μl of fetal bovine serum was used as positive control (Figs. 6A–B, FBS lane).
Affiliation: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Üllői út 26, 1085 Budapest, Hungary. firstname.lastname@example.org