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Roles of periostin in symptom manifestation and airway remodeling in a murine model of allergic rhinitis.

Hur DG, Khalmuratova R, Ahn SK, Ha YS, Min YG - Allergy Asthma Immunol Res (2012)

Bottom Line: No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group.However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group.The number of eosinophils and the symptom score were also lower in the knockout group.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Purpose: Periostin was originally identified as a secreted factor during screening of a mouse osteoblastic library. In a recent study, periostin was found to directly regulate eosinophil accumulation in allergic mucosal inflammation. Chronic eosinophilic inflammation is related to the development of remodeling. The present study examined the expression of periostin and evaluated its role in the inflammatory process and remodeling associated with allergic rhinitis.

Methods: A murine model of allergic rhinitis was established in periostin knockout mice. We analyzed the expression of periostin, manifestation of nasal symptoms, eosinophilic inflammation, and subepithelial fibrosis as well as the expression of MMP-2, TIMP-1, and type 1 collagen in nasal tissue.

Results: Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa. The subepithelial tissue was thinner in the knockout group than in the control group. No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group. However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group. The number of eosinophils and the symptom score were also lower in the knockout group.

Conclusions: Periostin is expressed in nasal tissues of murine models of allergic rhinitis. Periostin deficiency may affect the remodeling of nasal tissue with reduced subepithelial fibrosis, and lead to less eosinophilic inflammation.

No MeSH data available.


Related in: MedlinePlus

Eosinophil counts in the nasal mucosa. The nasal tissue around conchal cartilage in the OVA group after (A) 19 days, (B) 1 month, and (C) 3 months are shown. Eosinophils are reddish (arrow). The nasal tissue of the KO group after (D) 19 days, (E) 1 month, and (F) 3 months are also shown (Luna staining, original magnification, ×400). (G) The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA groups. *P<0.05. In the OVA group, the number of eosinophils was higher at 3 months of allergen challenge than at the other time points. **P<0.05. Data are expressed as mean±SE.HPF, high power field.
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Figure 7: Eosinophil counts in the nasal mucosa. The nasal tissue around conchal cartilage in the OVA group after (A) 19 days, (B) 1 month, and (C) 3 months are shown. Eosinophils are reddish (arrow). The nasal tissue of the KO group after (D) 19 days, (E) 1 month, and (F) 3 months are also shown (Luna staining, original magnification, ×400). (G) The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA groups. *P<0.05. In the OVA group, the number of eosinophils was higher at 3 months of allergen challenge than at the other time points. **P<0.05. Data are expressed as mean±SE.HPF, high power field.

Mentions: The proinflammatory functions and the important role of eosinophils in chronic allergic diseases have been clearly elucidated.21 To determine whether periostin deficiency affects the tissue infiltration of eosinophils, these cells were counted in the histology slides after staining with Sirius red dye. The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA group (P<0.05; Fig. 7). The OVA and KO groups showed significantly increased numbers of eosinophils in the nasal mucosa compared to the PBS group, which showed few eosinophils in the nasal mucosa (P<0.05). In the OVA group, the eosinophil count increased after 3 months of allergen challenge when compared to the previous time points (P<0.05).


Roles of periostin in symptom manifestation and airway remodeling in a murine model of allergic rhinitis.

Hur DG, Khalmuratova R, Ahn SK, Ha YS, Min YG - Allergy Asthma Immunol Res (2012)

Eosinophil counts in the nasal mucosa. The nasal tissue around conchal cartilage in the OVA group after (A) 19 days, (B) 1 month, and (C) 3 months are shown. Eosinophils are reddish (arrow). The nasal tissue of the KO group after (D) 19 days, (E) 1 month, and (F) 3 months are also shown (Luna staining, original magnification, ×400). (G) The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA groups. *P<0.05. In the OVA group, the number of eosinophils was higher at 3 months of allergen challenge than at the other time points. **P<0.05. Data are expressed as mean±SE.HPF, high power field.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378929&req=5

Figure 7: Eosinophil counts in the nasal mucosa. The nasal tissue around conchal cartilage in the OVA group after (A) 19 days, (B) 1 month, and (C) 3 months are shown. Eosinophils are reddish (arrow). The nasal tissue of the KO group after (D) 19 days, (E) 1 month, and (F) 3 months are also shown (Luna staining, original magnification, ×400). (G) The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA groups. *P<0.05. In the OVA group, the number of eosinophils was higher at 3 months of allergen challenge than at the other time points. **P<0.05. Data are expressed as mean±SE.HPF, high power field.
Mentions: The proinflammatory functions and the important role of eosinophils in chronic allergic diseases have been clearly elucidated.21 To determine whether periostin deficiency affects the tissue infiltration of eosinophils, these cells were counted in the histology slides after staining with Sirius red dye. The number of eosinophils in the nasal mucosa was significantly lower in the KO group than in the OVA group (P<0.05; Fig. 7). The OVA and KO groups showed significantly increased numbers of eosinophils in the nasal mucosa compared to the PBS group, which showed few eosinophils in the nasal mucosa (P<0.05). In the OVA group, the eosinophil count increased after 3 months of allergen challenge when compared to the previous time points (P<0.05).

Bottom Line: No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group.However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group.The number of eosinophils and the symptom score were also lower in the knockout group.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Purpose: Periostin was originally identified as a secreted factor during screening of a mouse osteoblastic library. In a recent study, periostin was found to directly regulate eosinophil accumulation in allergic mucosal inflammation. Chronic eosinophilic inflammation is related to the development of remodeling. The present study examined the expression of periostin and evaluated its role in the inflammatory process and remodeling associated with allergic rhinitis.

Methods: A murine model of allergic rhinitis was established in periostin knockout mice. We analyzed the expression of periostin, manifestation of nasal symptoms, eosinophilic inflammation, and subepithelial fibrosis as well as the expression of MMP-2, TIMP-1, and type 1 collagen in nasal tissue.

Results: Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa. The subepithelial tissue was thinner in the knockout group than in the control group. No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group. However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group. The number of eosinophils and the symptom score were also lower in the knockout group.

Conclusions: Periostin is expressed in nasal tissues of murine models of allergic rhinitis. Periostin deficiency may affect the remodeling of nasal tissue with reduced subepithelial fibrosis, and lead to less eosinophilic inflammation.

No MeSH data available.


Related in: MedlinePlus