Limits...
Roles of periostin in symptom manifestation and airway remodeling in a murine model of allergic rhinitis.

Hur DG, Khalmuratova R, Ahn SK, Ha YS, Min YG - Allergy Asthma Immunol Res (2012)

Bottom Line: No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group.However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group.The number of eosinophils and the symptom score were also lower in the knockout group.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Purpose: Periostin was originally identified as a secreted factor during screening of a mouse osteoblastic library. In a recent study, periostin was found to directly regulate eosinophil accumulation in allergic mucosal inflammation. Chronic eosinophilic inflammation is related to the development of remodeling. The present study examined the expression of periostin and evaluated its role in the inflammatory process and remodeling associated with allergic rhinitis.

Methods: A murine model of allergic rhinitis was established in periostin knockout mice. We analyzed the expression of periostin, manifestation of nasal symptoms, eosinophilic inflammation, and subepithelial fibrosis as well as the expression of MMP-2, TIMP-1, and type 1 collagen in nasal tissue.

Results: Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa. The subepithelial tissue was thinner in the knockout group than in the control group. No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group. However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group. The number of eosinophils and the symptom score were also lower in the knockout group.

Conclusions: Periostin is expressed in nasal tissues of murine models of allergic rhinitis. Periostin deficiency may affect the remodeling of nasal tissue with reduced subepithelial fibrosis, and lead to less eosinophilic inflammation.

No MeSH data available.


Related in: MedlinePlus

Periostin immunohistochemistry. Images show the nasal respiratory mucosa after 3 months of allergen challenge. (A) OVA group, original magnification, ×20. (B) OVA group, original magnification, ×400. (C) PBS group, original magnification, ×400. (D) KO group, original magnification, ×400. Periostin was highly expressed in the subepithelial area in the OVA group (asterisk in B). However, it was less expressed in the same area of the PBS group, and no periostin expression was observed in the KO group.PBS, phosphate buffered saline; OVA, ovalbumin; KO, knockout.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3378929&req=5

Figure 2: Periostin immunohistochemistry. Images show the nasal respiratory mucosa after 3 months of allergen challenge. (A) OVA group, original magnification, ×20. (B) OVA group, original magnification, ×400. (C) PBS group, original magnification, ×400. (D) KO group, original magnification, ×400. Periostin was highly expressed in the subepithelial area in the OVA group (asterisk in B). However, it was less expressed in the same area of the PBS group, and no periostin expression was observed in the KO group.PBS, phosphate buffered saline; OVA, ovalbumin; KO, knockout.

Mentions: Immunohistochemical staining was performed to determine the expression of periostin and its localization in the nasal tissue of AR mice. Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa as well as the pseudostratified columnar epithelium in the OVA group after 3 months of allergen challenge (Fig. 2). The area in which periostin was expressed nearly overlapped with the area that was stained by Masson's trichrome. Except for periosteal or perichondrial staining, areas positively stained for periostin were not identified in the KO and PBS groups.


Roles of periostin in symptom manifestation and airway remodeling in a murine model of allergic rhinitis.

Hur DG, Khalmuratova R, Ahn SK, Ha YS, Min YG - Allergy Asthma Immunol Res (2012)

Periostin immunohistochemistry. Images show the nasal respiratory mucosa after 3 months of allergen challenge. (A) OVA group, original magnification, ×20. (B) OVA group, original magnification, ×400. (C) PBS group, original magnification, ×400. (D) KO group, original magnification, ×400. Periostin was highly expressed in the subepithelial area in the OVA group (asterisk in B). However, it was less expressed in the same area of the PBS group, and no periostin expression was observed in the KO group.PBS, phosphate buffered saline; OVA, ovalbumin; KO, knockout.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378929&req=5

Figure 2: Periostin immunohistochemistry. Images show the nasal respiratory mucosa after 3 months of allergen challenge. (A) OVA group, original magnification, ×20. (B) OVA group, original magnification, ×400. (C) PBS group, original magnification, ×400. (D) KO group, original magnification, ×400. Periostin was highly expressed in the subepithelial area in the OVA group (asterisk in B). However, it was less expressed in the same area of the PBS group, and no periostin expression was observed in the KO group.PBS, phosphate buffered saline; OVA, ovalbumin; KO, knockout.
Mentions: Immunohistochemical staining was performed to determine the expression of periostin and its localization in the nasal tissue of AR mice. Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa as well as the pseudostratified columnar epithelium in the OVA group after 3 months of allergen challenge (Fig. 2). The area in which periostin was expressed nearly overlapped with the area that was stained by Masson's trichrome. Except for periosteal or perichondrial staining, areas positively stained for periostin were not identified in the KO and PBS groups.

Bottom Line: No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group.However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group.The number of eosinophils and the symptom score were also lower in the knockout group.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea.

ABSTRACT

Purpose: Periostin was originally identified as a secreted factor during screening of a mouse osteoblastic library. In a recent study, periostin was found to directly regulate eosinophil accumulation in allergic mucosal inflammation. Chronic eosinophilic inflammation is related to the development of remodeling. The present study examined the expression of periostin and evaluated its role in the inflammatory process and remodeling associated with allergic rhinitis.

Methods: A murine model of allergic rhinitis was established in periostin knockout mice. We analyzed the expression of periostin, manifestation of nasal symptoms, eosinophilic inflammation, and subepithelial fibrosis as well as the expression of MMP-2, TIMP-1, and type 1 collagen in nasal tissue.

Results: Periostin was mainly distributed in the subepithelial tissue of the nasal mucosa. The subepithelial tissue was thinner in the knockout group than in the control group. No differences in the expression of MMP-2 or TIMP-1 were found in the knockout group. However, after a month of allergen challenge, type I collagen in the nasal tissue was lower in the knockout group than in the control group. The number of eosinophils and the symptom score were also lower in the knockout group.

Conclusions: Periostin is expressed in nasal tissues of murine models of allergic rhinitis. Periostin deficiency may affect the remodeling of nasal tissue with reduced subepithelial fibrosis, and lead to less eosinophilic inflammation.

No MeSH data available.


Related in: MedlinePlus