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Therapeutic effects of mycobacterial secretory proteins against established asthma in BALB/c mice.

Han ER, Choi IS, Choi HG, Kim HJ - Allergy Asthma Immunol Res (2012)

Bottom Line: BCG and all of the tested secretory proteins significantly improved airway sensitivity compared to baseline values (P<0.05).The secretory protein Ag85 complex significantly suppressed airway reactivity also (P<0.05), while 38-kDa protein significantly suppressed reactivity and maximal narrowing (P<0.05).Thus, allergic asthma could be effectively treated with mycobacterial secretory proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Chonnam National University Medical School, Gwangju, Korea.

ABSTRACT

Purpose: Live/killed mycobacteria and culture supernatants can suppress asthmatic reactions. This study investigated whether mycobacterial secretory proteins have therapeutic effects on asthma.

Methods: Mycobacterium bovis bacille Calmette-Guérin (BCG; 2×10(5) CFUs) and mycobacterial secretory proteins (Ag85 complex, 38-kDa protein or MPB70; 4 or 20 µg) were administered intraperitoneally to female BALB/c mice with established airway hyperresponsiveness. One week after treatment, the mice underwent a methacholine challenge test, and then inflammatory cell numbers in bronchoalveolar lavage fluid (BAL) and around bronchi (<500 µm), and cytokine levels in splenocyte supernatants, were assessed.

Results: BCG and all of the tested secretory proteins significantly improved airway sensitivity compared to baseline values (P<0.05). The secretory protein Ag85 complex significantly suppressed airway reactivity also (P<0.05), while 38-kDa protein significantly suppressed reactivity and maximal narrowing (P<0.05). The number of eosinophils in BAL and around bronchi, and the goblet cell proportion, were also significantly reduced in mice in both the BCG and secretory protein groups compared to the asthma control group. IFN-γ/IL-5 ratios were significantly higher in mice treated with BCG, 4 µg MPB70 or 4 µg 38-kDa protein than in asthma control mice (P<0.05), and were negatively associated with airway hyperresponsiveness, peribronchial eosinophil numbers and goblet cell proportion (all P<0.05). IL-17A was positively correlated with IL-5 (r=0.379, P<0.001), maximal airway narrowing, peribronchial eosinophil numbers and goblet cell proportion (all P<0.05).

Conclusions: Secretory proteins from BCG and M. tuberculosis and live BCG were effective against established asthma, their effects being accompanied by increased IFN-γ/IL-5 ratios. Thus, allergic asthma could be effectively treated with mycobacterial secretory proteins.

No MeSH data available.


Related in: MedlinePlus

PC200 was significantly higher in all treatment groups than in the asthma control group. PC200: concentration of methacholine required for a 200% increase in enhanced pause index from baseline. *P<0.05, †P<0.001 vs. the asthma control group.
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Figure 2: PC200 was significantly higher in all treatment groups than in the asthma control group. PC200: concentration of methacholine required for a 200% increase in enhanced pause index from baseline. *P<0.05, †P<0.001 vs. the asthma control group.

Mentions: The PC200 value after treatment was significantly lower in the asthma control group than in the normal control group (Fig. 2). The PC200 values in all treatment groups were significantly higher than in the asthma control group. Similarly, the maximal Penh and DRS values were significantly higher in the asthma control group than in the normal control group. In addition, the values in the treatment groups, except for maximal Penh values in the 4 µg MPB70 and 20 µg 38-kDa protein groups and for DRS values in the 20 µg MPB70 and 20 µg Ag85 groups, were significantly lower than those in the asthma control group. Compared to the normal control group, all treatment groups showed significant differences in PC200 and maximal Penh values. However, the DRS values for the BCG, 4 µg Ag85, and 4 µg 38-kDa protein groups were not significantly different than those for the normal control group.


Therapeutic effects of mycobacterial secretory proteins against established asthma in BALB/c mice.

Han ER, Choi IS, Choi HG, Kim HJ - Allergy Asthma Immunol Res (2012)

PC200 was significantly higher in all treatment groups than in the asthma control group. PC200: concentration of methacholine required for a 200% increase in enhanced pause index from baseline. *P<0.05, †P<0.001 vs. the asthma control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378928&req=5

Figure 2: PC200 was significantly higher in all treatment groups than in the asthma control group. PC200: concentration of methacholine required for a 200% increase in enhanced pause index from baseline. *P<0.05, †P<0.001 vs. the asthma control group.
Mentions: The PC200 value after treatment was significantly lower in the asthma control group than in the normal control group (Fig. 2). The PC200 values in all treatment groups were significantly higher than in the asthma control group. Similarly, the maximal Penh and DRS values were significantly higher in the asthma control group than in the normal control group. In addition, the values in the treatment groups, except for maximal Penh values in the 4 µg MPB70 and 20 µg 38-kDa protein groups and for DRS values in the 20 µg MPB70 and 20 µg Ag85 groups, were significantly lower than those in the asthma control group. Compared to the normal control group, all treatment groups showed significant differences in PC200 and maximal Penh values. However, the DRS values for the BCG, 4 µg Ag85, and 4 µg 38-kDa protein groups were not significantly different than those for the normal control group.

Bottom Line: BCG and all of the tested secretory proteins significantly improved airway sensitivity compared to baseline values (P<0.05).The secretory protein Ag85 complex significantly suppressed airway reactivity also (P<0.05), while 38-kDa protein significantly suppressed reactivity and maximal narrowing (P<0.05).Thus, allergic asthma could be effectively treated with mycobacterial secretory proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Allergy, Chonnam National University Medical School, Gwangju, Korea.

ABSTRACT

Purpose: Live/killed mycobacteria and culture supernatants can suppress asthmatic reactions. This study investigated whether mycobacterial secretory proteins have therapeutic effects on asthma.

Methods: Mycobacterium bovis bacille Calmette-Guérin (BCG; 2×10(5) CFUs) and mycobacterial secretory proteins (Ag85 complex, 38-kDa protein or MPB70; 4 or 20 µg) were administered intraperitoneally to female BALB/c mice with established airway hyperresponsiveness. One week after treatment, the mice underwent a methacholine challenge test, and then inflammatory cell numbers in bronchoalveolar lavage fluid (BAL) and around bronchi (<500 µm), and cytokine levels in splenocyte supernatants, were assessed.

Results: BCG and all of the tested secretory proteins significantly improved airway sensitivity compared to baseline values (P<0.05). The secretory protein Ag85 complex significantly suppressed airway reactivity also (P<0.05), while 38-kDa protein significantly suppressed reactivity and maximal narrowing (P<0.05). The number of eosinophils in BAL and around bronchi, and the goblet cell proportion, were also significantly reduced in mice in both the BCG and secretory protein groups compared to the asthma control group. IFN-γ/IL-5 ratios were significantly higher in mice treated with BCG, 4 µg MPB70 or 4 µg 38-kDa protein than in asthma control mice (P<0.05), and were negatively associated with airway hyperresponsiveness, peribronchial eosinophil numbers and goblet cell proportion (all P<0.05). IL-17A was positively correlated with IL-5 (r=0.379, P<0.001), maximal airway narrowing, peribronchial eosinophil numbers and goblet cell proportion (all P<0.05).

Conclusions: Secretory proteins from BCG and M. tuberculosis and live BCG were effective against established asthma, their effects being accompanied by increased IFN-γ/IL-5 ratios. Thus, allergic asthma could be effectively treated with mycobacterial secretory proteins.

No MeSH data available.


Related in: MedlinePlus