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Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining of lung tissue for IL-5. IL-5-positive cells (black arrows) were significantly increased in the OVA+ASD group as compared with the other groups (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin; HPF, high-power field.
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Figure 6: Immunohistochemical staining of lung tissue for IL-5. IL-5-positive cells (black arrows) were significantly increased in the OVA+ASD group as compared with the other groups (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin; HPF, high-power field.

Mentions: The number of IL-4-positive cells was higher in groups 2, 3, and 4 than in group 1. In addition, this number was higher in group 4 (14.2±10.0) than in group 3 (2.4±2.3) (P<0.05; Fig. 5, Table 4). No IL-5-positive cells were observed in groups 1 and 2, but there were significantly more IL-5-positive cells in group 4 (68.4±20.0) than in group 3 (31.0±14.1) (P<0.05; Fig. 6). MUC5AC expression was not observed in groups 1 and 2; however, it was significantly higher in group 3 (7.4±5.3) and group 4 (25.8±3.7) (P<0.05), particularly in group 4 (Fig. 7). No TGF-α-positive cells were observed in groups 1 and 2, but the number of TGF-α-positive cells was significantly higher in group 4 (17.6±7.4) than in group 3 (3.6±2.4) (P<0.05; Fig. 8).


Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Immunohistochemical staining of lung tissue for IL-5. IL-5-positive cells (black arrows) were significantly increased in the OVA+ASD group as compared with the other groups (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin; HPF, high-power field.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378927&req=5

Figure 6: Immunohistochemical staining of lung tissue for IL-5. IL-5-positive cells (black arrows) were significantly increased in the OVA+ASD group as compared with the other groups (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin; HPF, high-power field.
Mentions: The number of IL-4-positive cells was higher in groups 2, 3, and 4 than in group 1. In addition, this number was higher in group 4 (14.2±10.0) than in group 3 (2.4±2.3) (P<0.05; Fig. 5, Table 4). No IL-5-positive cells were observed in groups 1 and 2, but there were significantly more IL-5-positive cells in group 4 (68.4±20.0) than in group 3 (31.0±14.1) (P<0.05; Fig. 6). MUC5AC expression was not observed in groups 1 and 2; however, it was significantly higher in group 3 (7.4±5.3) and group 4 (25.8±3.7) (P<0.05), particularly in group 4 (Fig. 7). No TGF-α-positive cells were observed in groups 1 and 2, but the number of TGF-α-positive cells was significantly higher in group 4 (17.6±7.4) than in group 3 (3.6±2.4) (P<0.05; Fig. 8).

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus