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Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus

Hematoxylin & eosin staining. Inflammatory cells in the bronchi were increased in the OVA and OVA+ASD groups as compared with the control group. Inflammatory cells (black arrows) were more prominent in the OVA+ASD group than in the OVA group (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin, HPF, high-power field.
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Figure 3: Hematoxylin & eosin staining. Inflammatory cells in the bronchi were increased in the OVA and OVA+ASD groups as compared with the control group. Inflammatory cells (black arrows) were more prominent in the OVA+ASD group than in the OVA group (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin, HPF, high-power field.

Mentions: The numbers of inflammatory cells in the bronchi were higher in groups 2 and 3 than in group 1, but the differences were not statistically significant. The average number of inflammatory cells was significantly higher in group 4 (233.4±31.0) than in group 3 (71.4±25.8) (P<0.05; Fig. 3). Treatment with OVA alone caused slight eosinophil infiltration, and OVA+ASD caused mild to moderate eosinophil infiltration in the airway (Table 4).


Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Hematoxylin & eosin staining. Inflammatory cells in the bronchi were increased in the OVA and OVA+ASD groups as compared with the control group. Inflammatory cells (black arrows) were more prominent in the OVA+ASD group than in the OVA group (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin, HPF, high-power field.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378927&req=5

Figure 3: Hematoxylin & eosin staining. Inflammatory cells in the bronchi were increased in the OVA and OVA+ASD groups as compared with the control group. Inflammatory cells (black arrows) were more prominent in the OVA+ASD group than in the OVA group (original magnification ×200, A: Control, B: ASD, C: OVA, D: OVA+ASD). *P<0.05 vs. the control group, †P<0.05 vs. the ASD group, ‡P<0.05 vs. the OVA group.ASD, Asian sand dust; OVA, ovalbumin, HPF, high-power field.
Mentions: The numbers of inflammatory cells in the bronchi were higher in groups 2 and 3 than in group 1, but the differences were not statistically significant. The average number of inflammatory cells was significantly higher in group 4 (233.4±31.0) than in group 3 (71.4±25.8) (P<0.05; Fig. 3). Treatment with OVA alone caused slight eosinophil infiltration, and OVA+ASD caused mild to moderate eosinophil infiltration in the airway (Table 4).

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus