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Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus

Allergic mouse model. After intraperitoneal injection of a mixture of 25 µg OVA and 1 mg Al(OH)3 gel, mice were nebulized with 2% OVA for 4 days. Mice were then stimulated with continuous 2% OVA alone (OVA group) or a mixture of 2% OVA and ASD (OVA+ASD group).IP, intraperitoneal; ASD, Asian sand dust; OVA, ovalbumin.
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Figure 1: Allergic mouse model. After intraperitoneal injection of a mixture of 25 µg OVA and 1 mg Al(OH)3 gel, mice were nebulized with 2% OVA for 4 days. Mice were then stimulated with continuous 2% OVA alone (OVA group) or a mixture of 2% OVA and ASD (OVA+ASD group).IP, intraperitoneal; ASD, Asian sand dust; OVA, ovalbumin.

Mentions: A total of 40 female BALB/c mice (6 weeks of age) were purchased (Central Lab., Animal Inc., Seoul, Korea) and checked for infections. The cages were placed in a conventional room maintained at 20-23℃ with 45%-70% humidity. This study adhered to the Gachon University Animal Research Guidelines. The mice in group 1 (G1, n=10) inhaled 1 mL normal saline through a nebulizer (Omron, Japan) for 15 minutes each day during the 7 day study period. Group 2 (G2, n=10) mice inhaled 1 mL of a mixture containing 10 mg ASD and 1 mL normal saline (ASD concentration, 10 mg/mL) using a nebulizer for 15 minutes each day during the 7 day study period. To develop an allergic mouse model, 25 µg OVA (OVA Sigma, St. Louis, MO) and 1 mg Al(OH)3 gel (Alum, Pierce Chemical Co., Rockford, IL, USA) were mixed in 3 mL normal saline. To perform systemic sensitization, 300 µL of the OVA mixture was injected into the mouse abdominal cavity on the 1st, 7th, and 14th days. The systemically sensitized mice were locally nebulized with 2% OVA for 30 minutes, four times daily starting on the 21st day. Local sensitization was performed in a 40×40×30 cm acrylic box, which had a side hole connected to the nebulizer, and allergic mice were then divided into group 3 (G3, n=10) and group 4 (G4, n=10). Group 3 mice inhaled 5 mL 2% OVA once every two days (30 minutes each) to sustain the allergic state. Group 4 mice inhaled both 5 mL 2% OVA and 1 mL of a mixture containing ASD and saline (10 mg ASD in 1 mL normal saline) for 30 minutes each day during the 7 day study period (Fig. 1).


Asian sand dust enhances allergen-induced th2 allergic inflammatory changes and mucin production in BALB/c mouse lungs.

Kang IG, Jung JH, Kim ST - Allergy Asthma Immunol Res (2012)

Allergic mouse model. After intraperitoneal injection of a mixture of 25 µg OVA and 1 mg Al(OH)3 gel, mice were nebulized with 2% OVA for 4 days. Mice were then stimulated with continuous 2% OVA alone (OVA group) or a mixture of 2% OVA and ASD (OVA+ASD group).IP, intraperitoneal; ASD, Asian sand dust; OVA, ovalbumin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3378927&req=5

Figure 1: Allergic mouse model. After intraperitoneal injection of a mixture of 25 µg OVA and 1 mg Al(OH)3 gel, mice were nebulized with 2% OVA for 4 days. Mice were then stimulated with continuous 2% OVA alone (OVA group) or a mixture of 2% OVA and ASD (OVA+ASD group).IP, intraperitoneal; ASD, Asian sand dust; OVA, ovalbumin.
Mentions: A total of 40 female BALB/c mice (6 weeks of age) were purchased (Central Lab., Animal Inc., Seoul, Korea) and checked for infections. The cages were placed in a conventional room maintained at 20-23℃ with 45%-70% humidity. This study adhered to the Gachon University Animal Research Guidelines. The mice in group 1 (G1, n=10) inhaled 1 mL normal saline through a nebulizer (Omron, Japan) for 15 minutes each day during the 7 day study period. Group 2 (G2, n=10) mice inhaled 1 mL of a mixture containing 10 mg ASD and 1 mL normal saline (ASD concentration, 10 mg/mL) using a nebulizer for 15 minutes each day during the 7 day study period. To develop an allergic mouse model, 25 µg OVA (OVA Sigma, St. Louis, MO) and 1 mg Al(OH)3 gel (Alum, Pierce Chemical Co., Rockford, IL, USA) were mixed in 3 mL normal saline. To perform systemic sensitization, 300 µL of the OVA mixture was injected into the mouse abdominal cavity on the 1st, 7th, and 14th days. The systemically sensitized mice were locally nebulized with 2% OVA for 30 minutes, four times daily starting on the 21st day. Local sensitization was performed in a 40×40×30 cm acrylic box, which had a side hole connected to the nebulizer, and allergic mice were then divided into group 3 (G3, n=10) and group 4 (G4, n=10). Group 3 mice inhaled 5 mL 2% OVA once every two days (30 minutes each) to sustain the allergic state. Group 4 mice inhaled both 5 mL 2% OVA and 1 mL of a mixture containing ASD and saline (10 mg ASD in 1 mL normal saline) for 30 minutes each day during the 7 day study period (Fig. 1).

Bottom Line: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05).Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05).The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.

ABSTRACT

Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice.

Methods: FORTY BALB/C FEMALE MICE WERE DIVIDED INTO FOUR GROUPS: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-α) was conducted.

Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-α-positive cells were also higher in group 4 than in group 3 (P<0.05).

Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.

No MeSH data available.


Related in: MedlinePlus