Morphometric classification and spatial organization of spiral ganglion neurons in the human cochlea: consequences for single fiber response to electrical stimulation.
Bottom Line: Furthermore, we found evidence of a spatial arrangement of perikarya and their accordant nuclei along the cochlea spiral.Cathodic stimulation was found to induce stronger variations of spikes while also leading to the lowest thresholds and longest latencies.Therefore, anodic stimulation leads to a more uniform excitation profile among SGCs with different cell body size.
Affiliation: Innsbruck Medical University, Department of Otorhinolaryngology, Laboratory for Inner Ear Biology, Anichstrasse 35, Innsbruck, Austria.Show MeSH
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Mentions: After being rinsed with PBS (3 × 10 min), sections were incubated with secondary antibodies conjugated to Alexa Fluor 488 (donkey anti-mouse, 1:1000, Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 633 (donkey anti-rabbit, 1:400, Invitrogen, Carlsbad, CA, USA), diluted in PBS for 2 h at RT and mounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA) for additional nuclei-counterstaining. Fig. 1 presents an example of immunolabeling of TuJ1 (green), S-100 (red) and DAPI (blue) staining of the human spiral ganglion. White arrows indicate cell-body candidates for volumetric reconstruction. Only perikarya completely surrounded by satellite glial cells without showing any shrinkage were chosen for three dimensional (3D) reconstructions.
Affiliation: Innsbruck Medical University, Department of Otorhinolaryngology, Laboratory for Inner Ear Biology, Anichstrasse 35, Innsbruck, Austria.