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Morphometric classification and spatial organization of spiral ganglion neurons in the human cochlea: consequences for single fiber response to electrical stimulation.

Potrusil T, Wenger C, Glueckert R, Schrott-Fischer A, Rattay F - Neuroscience (2012)

Bottom Line: Results show that temporal parameters of the spiking pattern are affected by the size of the cell body.Cathodic stimulation was found to induce stronger variations of spikes while also leading to the lowest thresholds and longest latencies.Therefore, anodic stimulation leads to a more uniform excitation profile among SGCs with different cell body size.

View Article: PubMed Central - PubMed

Affiliation: Innsbruck Medical University, Department of Otorhinolaryngology, Laboratory for Inner Ear Biology, Anichstrasse 35, Innsbruck, Austria.

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Immunolabeling for TuJ1 and S-100 in human spiral ganglion. SGCs are stained positively for TuJ1 (green). The satellite glial cells surrounding SGCs are strongly stained for S-100 (red). Nuclei of neurons and satellite cells are illustrated blue. White arrows indicate cell-body-candidates for volumetric reconstruction. Scale bar represents 20 μm.
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f0005: Immunolabeling for TuJ1 and S-100 in human spiral ganglion. SGCs are stained positively for TuJ1 (green). The satellite glial cells surrounding SGCs are strongly stained for S-100 (red). Nuclei of neurons and satellite cells are illustrated blue. White arrows indicate cell-body-candidates for volumetric reconstruction. Scale bar represents 20 μm.

Mentions: After being rinsed with PBS (3 × 10 min), sections were incubated with secondary antibodies conjugated to Alexa Fluor 488 (donkey anti-mouse, 1:1000, Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 633 (donkey anti-rabbit, 1:400, Invitrogen, Carlsbad, CA, USA), diluted in PBS for 2 h at RT and mounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA) for additional nuclei-counterstaining. Fig. 1 presents an example of immunolabeling of TuJ1 (green), S-100 (red) and DAPI (blue) staining of the human spiral ganglion. White arrows indicate cell-body candidates for volumetric reconstruction. Only perikarya completely surrounded by satellite glial cells without showing any shrinkage were chosen for three dimensional (3D) reconstructions.


Morphometric classification and spatial organization of spiral ganglion neurons in the human cochlea: consequences for single fiber response to electrical stimulation.

Potrusil T, Wenger C, Glueckert R, Schrott-Fischer A, Rattay F - Neuroscience (2012)

Immunolabeling for TuJ1 and S-100 in human spiral ganglion. SGCs are stained positively for TuJ1 (green). The satellite glial cells surrounding SGCs are strongly stained for S-100 (red). Nuclei of neurons and satellite cells are illustrated blue. White arrows indicate cell-body-candidates for volumetric reconstruction. Scale bar represents 20 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3377987&req=5

f0005: Immunolabeling for TuJ1 and S-100 in human spiral ganglion. SGCs are stained positively for TuJ1 (green). The satellite glial cells surrounding SGCs are strongly stained for S-100 (red). Nuclei of neurons and satellite cells are illustrated blue. White arrows indicate cell-body-candidates for volumetric reconstruction. Scale bar represents 20 μm.
Mentions: After being rinsed with PBS (3 × 10 min), sections were incubated with secondary antibodies conjugated to Alexa Fluor 488 (donkey anti-mouse, 1:1000, Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 633 (donkey anti-rabbit, 1:400, Invitrogen, Carlsbad, CA, USA), diluted in PBS for 2 h at RT and mounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA) for additional nuclei-counterstaining. Fig. 1 presents an example of immunolabeling of TuJ1 (green), S-100 (red) and DAPI (blue) staining of the human spiral ganglion. White arrows indicate cell-body candidates for volumetric reconstruction. Only perikarya completely surrounded by satellite glial cells without showing any shrinkage were chosen for three dimensional (3D) reconstructions.

Bottom Line: Results show that temporal parameters of the spiking pattern are affected by the size of the cell body.Cathodic stimulation was found to induce stronger variations of spikes while also leading to the lowest thresholds and longest latencies.Therefore, anodic stimulation leads to a more uniform excitation profile among SGCs with different cell body size.

View Article: PubMed Central - PubMed

Affiliation: Innsbruck Medical University, Department of Otorhinolaryngology, Laboratory for Inner Ear Biology, Anichstrasse 35, Innsbruck, Austria.

Show MeSH
Related in: MedlinePlus