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Evaluation of a biodegradable graft substitute in rabbit bone defect model.

Yang X, Li Y, Huang Q, Yang J, Shen B, Pei F - Indian J Orthop (2012)

Bottom Line: There was no significant difference between groups A and B.These findings demonstrated that the novel biodegradable copolymers can repair large areas of cancellous bone defects.With its controllable degradation rate, it suggests that CS/PAA may be a series of useful therapeutic substitute for bone defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, China.

ABSTRACT

Objective: To evaluate a new biodegradable copolymer calcium sulfate/poly amino acid (CS/PAA) as a graft substitute for the repair of the surgically created cancellous bone defects in rabbits and its biological properties in vivo.

Materials and methods: Cancellous bone defects were created by drilling holes in the unilateral lateral aspect of the femoral condyle of New Zealand white rabbits. Three groups were assigned: Group A rabbits were grafted with 80% CS/PAA and group B rabbits were grafted with 95% CS/PAA as two treatment groups; group C was sham-operation control group. To study the osteogenic capability in vivo, specimens were harvested at 4, 8, 12, and 16 weeks after implantation and were evaluated by gross assessment, X-ray, histological examination, and histomorphometry. In order to identify the molecular mechanism of bone defect repair, the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) was detected using Western blot at 4 weeks.

Results: Group A and group B showed more vigorous and rapid repair leading to regeneration of cancellous bone than sham-operation control group on gross observation, radiology, and histomorphometry. There was no significant difference between groups A and B. Morphological observation and histological examination showed that the copolymers degraded in sync with the new bone formation process. The expression of BMP-2 and VEGF in implantation groups was higher than that in control group by western blot.

Conclusion: These findings demonstrated that the novel biodegradable copolymers can repair large areas of cancellous bone defects. With its controllable degradation rate, it suggests that CS/PAA may be a series of useful therapeutic substitute for bone defects.

No MeSH data available.


Related in: MedlinePlus

Western blot analyses of the expression of the osteogenic growth factor bone morphogenetic protein-2 (BMP-2) and angiogenic growth factor vascular endothelial growth factor (VEGF). There was a significant difference between the two implantation groups and the control group (**P<0.01)
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Figure 7: Western blot analyses of the expression of the osteogenic growth factor bone morphogenetic protein-2 (BMP-2) and angiogenic growth factor vascular endothelial growth factor (VEGF). There was a significant difference between the two implantation groups and the control group (**P<0.01)

Mentions: During the natural process of bone defect healing, BMP-2 and VEGF are generally expressed at a high level in the early stage. In the present study, the expression of BMP-2 and VEGF in both treatment groups was upregulated compared with control group (P<0.01) at 4 weeks [Figure 7]. Between groups A and B, there was no significant difference in the expression of BMP-2 and VEGF (P>0.05).


Evaluation of a biodegradable graft substitute in rabbit bone defect model.

Yang X, Li Y, Huang Q, Yang J, Shen B, Pei F - Indian J Orthop (2012)

Western blot analyses of the expression of the osteogenic growth factor bone morphogenetic protein-2 (BMP-2) and angiogenic growth factor vascular endothelial growth factor (VEGF). There was a significant difference between the two implantation groups and the control group (**P<0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3377135&req=5

Figure 7: Western blot analyses of the expression of the osteogenic growth factor bone morphogenetic protein-2 (BMP-2) and angiogenic growth factor vascular endothelial growth factor (VEGF). There was a significant difference between the two implantation groups and the control group (**P<0.01)
Mentions: During the natural process of bone defect healing, BMP-2 and VEGF are generally expressed at a high level in the early stage. In the present study, the expression of BMP-2 and VEGF in both treatment groups was upregulated compared with control group (P<0.01) at 4 weeks [Figure 7]. Between groups A and B, there was no significant difference in the expression of BMP-2 and VEGF (P>0.05).

Bottom Line: There was no significant difference between groups A and B.These findings demonstrated that the novel biodegradable copolymers can repair large areas of cancellous bone defects.With its controllable degradation rate, it suggests that CS/PAA may be a series of useful therapeutic substitute for bone defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, China.

ABSTRACT

Objective: To evaluate a new biodegradable copolymer calcium sulfate/poly amino acid (CS/PAA) as a graft substitute for the repair of the surgically created cancellous bone defects in rabbits and its biological properties in vivo.

Materials and methods: Cancellous bone defects were created by drilling holes in the unilateral lateral aspect of the femoral condyle of New Zealand white rabbits. Three groups were assigned: Group A rabbits were grafted with 80% CS/PAA and group B rabbits were grafted with 95% CS/PAA as two treatment groups; group C was sham-operation control group. To study the osteogenic capability in vivo, specimens were harvested at 4, 8, 12, and 16 weeks after implantation and were evaluated by gross assessment, X-ray, histological examination, and histomorphometry. In order to identify the molecular mechanism of bone defect repair, the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) was detected using Western blot at 4 weeks.

Results: Group A and group B showed more vigorous and rapid repair leading to regeneration of cancellous bone than sham-operation control group on gross observation, radiology, and histomorphometry. There was no significant difference between groups A and B. Morphological observation and histological examination showed that the copolymers degraded in sync with the new bone formation process. The expression of BMP-2 and VEGF in implantation groups was higher than that in control group by western blot.

Conclusion: These findings demonstrated that the novel biodegradable copolymers can repair large areas of cancellous bone defects. With its controllable degradation rate, it suggests that CS/PAA may be a series of useful therapeutic substitute for bone defects.

No MeSH data available.


Related in: MedlinePlus