Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb.
Bottom Line: Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampal and OB-derived neural stem cells.We also show that adult neural progenitors derived from DB animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of DB animals reduces glucose levels.This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. email@example.comShow MeSH
Mentions: Adult NSCs were microinjected into adult mouse pancreas. Three weeks later, mice were injected with BrdU daily for 10 days. In the control (Control NSC TP), Sox2CreGFP+ cells co-localized with insulin (Fig 4A), C-peptide (Fig S7B of Supporting information) and NeuroD1 (Fig S7C of Supporting information). Interestingly, we found that Sox2CreGFP+ cells expressed MafA (Fig 4C) and pancreas transcription factor 1a (Ptf1a; Fig 4D). In microarray analysis (Fig 3C), adult NSCs did not express pancreatic β cells markers such as MafA. During embryonic development, lineage commitment of endocrine progenitors requires PTF1a (Kawaguchi et al, 2002). NSCs grafted in the pancreas, expressed β cell-specific markers such as PTF1a and MafA, suggesting that adult NSCs possessed the intrinsic ability to express these β cell-specific markers and that their expression levels were modulated by extracellular factors.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. firstname.lastname@example.org