Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb.
Bottom Line: Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampal and OB-derived neural stem cells.We also show that adult neural progenitors derived from DB animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of DB animals reduces glucose levels.This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. email@example.comShow MeSH
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Mentions: HPC NSCs were round and retained this shape when expanded as a monolayer (Fig 3A, left). OB NSCs grew as heterogeneous forms with adherent property and neurosphere morphologies (Fig 3A, right). Under neuron differentiation conditions, the cell morphologies changed markedly: both HPC and OB NSCs extended prolonged neurites (Fig 3B). Gene expression profiles were measured using Agilent rat genome microarrays (Table 1 of Supporting information). HPC NSC, OB NSC and HPC and OB neuron expression profiles were grouped by hierarchical clustering, and correlation coefficients were computed for all pair-wise comparisons (Fig S5 of Supporting information). Both HPC NSCs and OB NSCs expressed the NSC marker Sox2, the radial glial cell marker nestin and the neural progenitor markers Olig2 and Sox1 (NSC lineage; Fig 3C). NSC marker gene expression was down-regulated in HPC and OB neurons (HPC Neu and OB Neu in Fig 3C), and neuronal marker gene expression was up-regulated (mature neuron marker; Fig 3C), indicating their successful commitment into neuronal lineages.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. firstname.lastname@example.org