Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb.
Bottom Line: Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampal and OB-derived neural stem cells.We also show that adult neural progenitors derived from DB animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of DB animals reduces glucose levels.This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. firstname.lastname@example.orgShow MeSH
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Mentions: Because neurons produced insulin (Fig 1), we were interested in the niches that supported neuronal differentiation. Astrocytes define the HPC niche (Song et al, 2002), and astrocyte-secreting Wnt3 factors (Fig S4A of Supporting information) have instructive effects in promoting adult neurogenesis (Lie et al, 2005). Glial fibrillary acidic protein (GFAP) is an astrocyte marker, and GFAP-expressing (GFAP+) cells were detected in pancreatic α cells (Fig S4B of Supporting information). Interestingly, IHC revealed that the pancreatic GFAP+ cells co-localized with Wnt3+ cells (Fig 2A), indicating that α cells release the neurogenic Wnt3 as do hippocampal astrocytes.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. email@example.com