Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb.
Bottom Line: Paracrine Wnt3 plays an essential role in promoting the active expression of insulin in both hippocampal and OB-derived neural stem cells.We also show that adult neural progenitors derived from DB animals retain the ability to give rise to insulin-producing cells and that grafting neuronal progenitors into the pancreas of DB animals reduces glucose levels.This study provides an example of a simple and direct use of adult stem cells from one organ to another, without introducing additional inductive genes.
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. email@example.comShow MeSH
Mentions: We first investigated insulin-1 mRNA expression in adult HPC by in situ hybridization. Positive insulin-1 mRNA signals were detected at granule cell layers (GCL) in DG (Fig 1A; negative controls are shown in Fig S1A of Supporting information). Strong signals were observed in neuronal layers but were not found in cells at the inner layer of the DG where astrocytes and undifferentiated NSCs reside. Signals were also detected in CA1 and CA3 pyramidal neuron layers (Fig S1B of Supporting information).
Affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Science City, Japan. firstname.lastname@example.org