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SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.

Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M - EMBO Mol Med (2011)

Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.

View Article: PubMed Central - PubMed

Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. tmatsui@icems.kyoto-u.ac.jp

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Related in: MedlinePlus

Free amino acids in SC of SASP+/+ and SASP−/− miceTotal free amino acid composition analysis. Tape stripped SC of SASP+/+ (n = 7, filled column) and SASP−/− (n = 11, open column) hairless mice were subjected to amino acid analysis. Total free amino acid composition analyses showed no difference between the SC of SASP+/+ and SASP−/− mice. ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).Individual free amino acid composition analysis. Individual free amino acid composition analyses also showed no difference between the SC of SASP+/+(n = 7, filled columns) and SASP−/− mice (n = 11, open columns). ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).
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fig06: Free amino acids in SC of SASP+/+ and SASP−/− miceTotal free amino acid composition analysis. Tape stripped SC of SASP+/+ (n = 7, filled column) and SASP−/− (n = 11, open column) hairless mice were subjected to amino acid analysis. Total free amino acid composition analyses showed no difference between the SC of SASP+/+ and SASP−/− mice. ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).Individual free amino acid composition analysis. Individual free amino acid composition analyses also showed no difference between the SC of SASP+/+(n = 7, filled columns) and SASP−/− mice (n = 11, open columns). ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).

Mentions: Previous reports suggest that NMFs serve as natural humectants of SC (reviewed in Rawlings & Matts, 2005). To investigate whether aberrant processing of filaggrin had a detrimental effect on the composition of NMFs, we performed free amino acid analysis of tape stripped SC of SASP+/+ (n = 7) and SASP−/− (n = 11) mice. There were no alterations in the total amount or the composition of free amino acids (Fig 6A and B). These results suggest the NMFs were normal in SASP−/− SC, despite the marked decrease in SC hydration.


SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.

Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M - EMBO Mol Med (2011)

Free amino acids in SC of SASP+/+ and SASP−/− miceTotal free amino acid composition analysis. Tape stripped SC of SASP+/+ (n = 7, filled column) and SASP−/− (n = 11, open column) hairless mice were subjected to amino acid analysis. Total free amino acid composition analyses showed no difference between the SC of SASP+/+ and SASP−/− mice. ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).Individual free amino acid composition analysis. Individual free amino acid composition analyses also showed no difference between the SC of SASP+/+(n = 7, filled columns) and SASP−/− mice (n = 11, open columns). ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3377080&req=5

fig06: Free amino acids in SC of SASP+/+ and SASP−/− miceTotal free amino acid composition analysis. Tape stripped SC of SASP+/+ (n = 7, filled column) and SASP−/− (n = 11, open column) hairless mice were subjected to amino acid analysis. Total free amino acid composition analyses showed no difference between the SC of SASP+/+ and SASP−/− mice. ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).Individual free amino acid composition analysis. Individual free amino acid composition analyses also showed no difference between the SC of SASP+/+(n = 7, filled columns) and SASP−/− mice (n = 11, open columns). ns: not significant (Mann–Whitney test on mean values ± SD using Graphpad software).
Mentions: Previous reports suggest that NMFs serve as natural humectants of SC (reviewed in Rawlings & Matts, 2005). To investigate whether aberrant processing of filaggrin had a detrimental effect on the composition of NMFs, we performed free amino acid analysis of tape stripped SC of SASP+/+ (n = 7) and SASP−/− (n = 11) mice. There were no alterations in the total amount or the composition of free amino acids (Fig 6A and B). These results suggest the NMFs were normal in SASP−/− SC, despite the marked decrease in SC hydration.

Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.

View Article: PubMed Central - PubMed

Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. tmatsui@icems.kyoto-u.ac.jp

Show MeSH
Related in: MedlinePlus