SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.
Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.
Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. firstname.lastname@example.orgShow MeSH
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Mentions: Previous reports suggest that NMFs serve as natural humectants of SC (reviewed in Rawlings & Matts, 2005). To investigate whether aberrant processing of filaggrin had a detrimental effect on the composition of NMFs, we performed free amino acid analysis of tape stripped SC of SASP+/+ (n = 7) and SASP−/− (n = 11) mice. There were no alterations in the total amount or the composition of free amino acids (Fig 6A and B). These results suggest the NMFs were normal in SASP−/− SC, despite the marked decrease in SC hydration.
Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. email@example.com