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SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.

Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M - EMBO Mol Med (2011)

Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.

View Article: PubMed Central - PubMed

Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. tmatsui@icems.kyoto-u.ac.jp

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Related in: MedlinePlus

SASPase regulates SC hydrationTrans-epidermal water loss (TEWL) of SASP+/+ and SASP−/− hairless mice. TEWL of hairless mice were measured using VAVO SCAN (Asahi Biomed, Tokyo, Japan). The TEWL of SASP−/− hairless mice was not significantly changed. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. ns: not significant (Mann Whitney test on mean values ± SD using Graphpad software).SC hydration levels of SASP+/+ and SASP−/− hairless mice. SC hydration levels of hairless mice were measured using ASA-M1 (Asahi Biomed). The SC hydration of SASP−/− hairless mice was significantly lower than that of SASP+/+ hairless mice. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. The p value is indicated above the bar (Mann Whitney test on mean values ± SD using Graphpad software).
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fig03: SASPase regulates SC hydrationTrans-epidermal water loss (TEWL) of SASP+/+ and SASP−/− hairless mice. TEWL of hairless mice were measured using VAVO SCAN (Asahi Biomed, Tokyo, Japan). The TEWL of SASP−/− hairless mice was not significantly changed. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. ns: not significant (Mann Whitney test on mean values ± SD using Graphpad software).SC hydration levels of SASP+/+ and SASP−/− hairless mice. SC hydration levels of hairless mice were measured using ASA-M1 (Asahi Biomed). The SC hydration of SASP−/− hairless mice was significantly lower than that of SASP+/+ hairless mice. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. The p value is indicated above the bar (Mann Whitney test on mean values ± SD using Graphpad software).

Mentions: To physiologically characterize the epidermal surface of the SASP−/− hairless mice, we measured the trans-epidermal water loss (TEWL) and the SC hydration of SASP+/+ (n = 7) and SASP−/− (n = 11) mice (Fig 3A and B). Although the TEWL was not significantly changed between SASP+/+ and SASP−/− mice (Fig 3A), a clear difference was observed in SC hydration. As such, SC hydration of SASP−/− mice was significantly lower than in SASP+/+ mice (Fig 3B). These results indicate that SASP−/− hairless mice showed markedly decreased SC hydration without alteration of barrier function.


SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.

Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M - EMBO Mol Med (2011)

SASPase regulates SC hydrationTrans-epidermal water loss (TEWL) of SASP+/+ and SASP−/− hairless mice. TEWL of hairless mice were measured using VAVO SCAN (Asahi Biomed, Tokyo, Japan). The TEWL of SASP−/− hairless mice was not significantly changed. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. ns: not significant (Mann Whitney test on mean values ± SD using Graphpad software).SC hydration levels of SASP+/+ and SASP−/− hairless mice. SC hydration levels of hairless mice were measured using ASA-M1 (Asahi Biomed). The SC hydration of SASP−/− hairless mice was significantly lower than that of SASP+/+ hairless mice. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. The p value is indicated above the bar (Mann Whitney test on mean values ± SD using Graphpad software).
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Related In: Results  -  Collection

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fig03: SASPase regulates SC hydrationTrans-epidermal water loss (TEWL) of SASP+/+ and SASP−/− hairless mice. TEWL of hairless mice were measured using VAVO SCAN (Asahi Biomed, Tokyo, Japan). The TEWL of SASP−/− hairless mice was not significantly changed. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. ns: not significant (Mann Whitney test on mean values ± SD using Graphpad software).SC hydration levels of SASP+/+ and SASP−/− hairless mice. SC hydration levels of hairless mice were measured using ASA-M1 (Asahi Biomed). The SC hydration of SASP−/− hairless mice was significantly lower than that of SASP+/+ hairless mice. The numbers of animals tested were: SASP+/+, n = 7 and SASP−/−, n = 11. The p value is indicated above the bar (Mann Whitney test on mean values ± SD using Graphpad software).
Mentions: To physiologically characterize the epidermal surface of the SASP−/− hairless mice, we measured the trans-epidermal water loss (TEWL) and the SC hydration of SASP+/+ (n = 7) and SASP−/− (n = 11) mice (Fig 3A and B). Although the TEWL was not significantly changed between SASP+/+ and SASP−/− mice (Fig 3A), a clear difference was observed in SC hydration. As such, SC hydration of SASP−/− mice was significantly lower than in SASP+/+ mice (Fig 3B). These results indicate that SASP−/− hairless mice showed markedly decreased SC hydration without alteration of barrier function.

Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.

View Article: PubMed Central - PubMed

Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. tmatsui@icems.kyoto-u.ac.jp

Show MeSH
Related in: MedlinePlus