SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.
Bottom Line: Furthermore, missense mutations were detected in 5 of 196 atopic dermatitis (AD) patients and 2 of 28 normal individuals.Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.These findings indicate that SASPase activity is indispensable for processing profilaggrin and maintaining the texture and hydration of the SC.
Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. firstname.lastname@example.orgShow MeSH
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Mentions: To physiologically characterize the epidermal surface of the SASP−/− hairless mice, we measured the trans-epidermal water loss (TEWL) and the SC hydration of SASP+/+ (n = 7) and SASP−/− (n = 11) mice (Fig 3A and B). Although the TEWL was not significantly changed between SASP+/+ and SASP−/− mice (Fig 3A), a clear difference was observed in SC hydration. As such, SC hydration of SASP−/− mice was significantly lower than in SASP+/+ mice (Fig 3B). These results indicate that SASP−/− hairless mice showed markedly decreased SC hydration without alteration of barrier function.
Affiliation: Medical Top Track (MTT) Program, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan. email@example.com