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Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid).

Cattaneo E, Laczko E, Buffoli F, Zorzi F, Bianco MA, Menigatti M, Bartosova Z, Haider R, Helmchen B, Sabates-Bellver J, Tiwari A, Jiricny J, Marra G - EMBO Mol Med (2011)

Bottom Line: The latter also displayed fewer and less dramatic expression changes than polypoid lesions.This finding, along with TMIGD1 protein expression patterns in tissues and cell lines, suggests that TMIGD1 might be associated with intestinal-cell differentiation.We conclude that molecular dysregulation in slightly elevated, nonpolypoid, precancerous colorectal lesions may be somewhat less severe than that observed in classic adenomatous polyps.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Cancer Research, University of Zurich, Switzerland.

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TMIGD1 mRNA expression in colorectal tissues and cancer cell linesNormalized log2 expression intensity values of TMIGD1 mRNA detected by exon array analysis in polypoid and nonpolypoid precancerous lesions (green and blue dots, respectively). Grey dots: Corresponding values for six colorectal cancers recently investigated with the same arrays. Results are expressed as fold changes (FCs) versus values observed in corresponding samples of normal mucosa (red dots). Box plots show 25th, 50th (median), and 75th percentiles of lesional expression values.Real time quantitative RT-PCR confirmed TMIGD1 mRNA expression levels in the colorectal tissues investigated in the microarray study. Transcript was also detected in the normal terminal ileum and in several cell lines. Other cell lines (SW480, Colo741, HT29, HT29M6, Co115, SW48, CX1, SW837, HCT116, SW620, Vaco481, SW403, LnCaP, MCF7, Hela, BEAS2B, U2OS, HEK293T, A2780, LAN-1, and Sk-N-SH) did not express TMIGD1. TMIGD1 (target) and GAPDH (reference) coding regions were amplified. y-axis: [target concentration/reference concentration] for sample/[target concentration/reference concentration] for normal mucosa.
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fig05: TMIGD1 mRNA expression in colorectal tissues and cancer cell linesNormalized log2 expression intensity values of TMIGD1 mRNA detected by exon array analysis in polypoid and nonpolypoid precancerous lesions (green and blue dots, respectively). Grey dots: Corresponding values for six colorectal cancers recently investigated with the same arrays. Results are expressed as fold changes (FCs) versus values observed in corresponding samples of normal mucosa (red dots). Box plots show 25th, 50th (median), and 75th percentiles of lesional expression values.Real time quantitative RT-PCR confirmed TMIGD1 mRNA expression levels in the colorectal tissues investigated in the microarray study. Transcript was also detected in the normal terminal ileum and in several cell lines. Other cell lines (SW480, Colo741, HT29, HT29M6, Co115, SW48, CX1, SW837, HCT116, SW620, Vaco481, SW403, LnCaP, MCF7, Hela, BEAS2B, U2OS, HEK293T, A2780, LAN-1, and Sk-N-SH) did not express TMIGD1. TMIGD1 (target) and GAPDH (reference) coding regions were amplified. y-axis: [target concentration/reference concentration] for sample/[target concentration/reference concentration] for normal mucosa.

Mentions: TMIGD1 ranked very high on the list of genes whose expression levels varied markedly with tissue type (Fig 2B), lesion size, and histology (Fig. .3; Supporting Information Table 2). The constant representation of this gene in the multivariate analyses reported thus far reflects its progressive downregulation as transformation advances. As shown in Fig 5A, its expression was clearly decreased in nonpolypoid lesions (fold-change; FC vs. normal mucosa: −19), but the downregulation was much more evident in polypoid lesions (FC −66) and even more dramatic in a small series of advanced cancers (FC −125) we recently examined with exon arrays.


Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid).

Cattaneo E, Laczko E, Buffoli F, Zorzi F, Bianco MA, Menigatti M, Bartosova Z, Haider R, Helmchen B, Sabates-Bellver J, Tiwari A, Jiricny J, Marra G - EMBO Mol Med (2011)

TMIGD1 mRNA expression in colorectal tissues and cancer cell linesNormalized log2 expression intensity values of TMIGD1 mRNA detected by exon array analysis in polypoid and nonpolypoid precancerous lesions (green and blue dots, respectively). Grey dots: Corresponding values for six colorectal cancers recently investigated with the same arrays. Results are expressed as fold changes (FCs) versus values observed in corresponding samples of normal mucosa (red dots). Box plots show 25th, 50th (median), and 75th percentiles of lesional expression values.Real time quantitative RT-PCR confirmed TMIGD1 mRNA expression levels in the colorectal tissues investigated in the microarray study. Transcript was also detected in the normal terminal ileum and in several cell lines. Other cell lines (SW480, Colo741, HT29, HT29M6, Co115, SW48, CX1, SW837, HCT116, SW620, Vaco481, SW403, LnCaP, MCF7, Hela, BEAS2B, U2OS, HEK293T, A2780, LAN-1, and Sk-N-SH) did not express TMIGD1. TMIGD1 (target) and GAPDH (reference) coding regions were amplified. y-axis: [target concentration/reference concentration] for sample/[target concentration/reference concentration] for normal mucosa.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3377079&req=5

fig05: TMIGD1 mRNA expression in colorectal tissues and cancer cell linesNormalized log2 expression intensity values of TMIGD1 mRNA detected by exon array analysis in polypoid and nonpolypoid precancerous lesions (green and blue dots, respectively). Grey dots: Corresponding values for six colorectal cancers recently investigated with the same arrays. Results are expressed as fold changes (FCs) versus values observed in corresponding samples of normal mucosa (red dots). Box plots show 25th, 50th (median), and 75th percentiles of lesional expression values.Real time quantitative RT-PCR confirmed TMIGD1 mRNA expression levels in the colorectal tissues investigated in the microarray study. Transcript was also detected in the normal terminal ileum and in several cell lines. Other cell lines (SW480, Colo741, HT29, HT29M6, Co115, SW48, CX1, SW837, HCT116, SW620, Vaco481, SW403, LnCaP, MCF7, Hela, BEAS2B, U2OS, HEK293T, A2780, LAN-1, and Sk-N-SH) did not express TMIGD1. TMIGD1 (target) and GAPDH (reference) coding regions were amplified. y-axis: [target concentration/reference concentration] for sample/[target concentration/reference concentration] for normal mucosa.
Mentions: TMIGD1 ranked very high on the list of genes whose expression levels varied markedly with tissue type (Fig 2B), lesion size, and histology (Fig. .3; Supporting Information Table 2). The constant representation of this gene in the multivariate analyses reported thus far reflects its progressive downregulation as transformation advances. As shown in Fig 5A, its expression was clearly decreased in nonpolypoid lesions (fold-change; FC vs. normal mucosa: −19), but the downregulation was much more evident in polypoid lesions (FC −66) and even more dramatic in a small series of advanced cancers (FC −125) we recently examined with exon arrays.

Bottom Line: The latter also displayed fewer and less dramatic expression changes than polypoid lesions.This finding, along with TMIGD1 protein expression patterns in tissues and cell lines, suggests that TMIGD1 might be associated with intestinal-cell differentiation.We conclude that molecular dysregulation in slightly elevated, nonpolypoid, precancerous colorectal lesions may be somewhat less severe than that observed in classic adenomatous polyps.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Cancer Research, University of Zurich, Switzerland.

Show MeSH
Related in: MedlinePlus