Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid).
Bottom Line: The latter also displayed fewer and less dramatic expression changes than polypoid lesions.This finding, along with TMIGD1 protein expression patterns in tissues and cell lines, suggests that TMIGD1 might be associated with intestinal-cell differentiation.We conclude that molecular dysregulation in slightly elevated, nonpolypoid, precancerous colorectal lesions may be somewhat less severe than that observed in classic adenomatous polyps.
Affiliation: Institute of Molecular Cancer Research, University of Zurich, Switzerland.Show MeSH
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Mentions: Expression of 23,768 transcript clusters was analysed in all 42 precancerous lesions and their corresponding samples of normal mucosa (see Materials and Methods and Supporting Information). Unsupervised principal component analysis (PCA; Fig 1A) demonstrated that much of the variance among the samples was due to interindividual variability (PC axis 1, PC1), but a considerable portion of the residual variance was accounted for by tissue type (i.e. normal mucosa, polypoid lesions and nonpolypoid lesions; PC2). This variable was therefore used as the prime grouping factor in the supervised between-group analysis (BGA) based on correspondence analysis (CoA; Fig 1B). Normal mucosa samples were clearly segregated from the precancerous lesions, and the polypoid and nonpolypoid subsets within the latter category were also clearly distinct. [These distinctions were still evident after exclusion from the BGA of the three nondysplastic serrated lesions (Supporting Information Fig 1).]
Affiliation: Institute of Molecular Cancer Research, University of Zurich, Switzerland.